New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	4808173 =	0 (0.000)	8 (0.140)	7/252	NT	25.4%	intergenic (‑282/‑273)	PP_4234/dsbD‑II	bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
	?	NC_002947	= 4945372	47 (0.810)					intergenic (+732/+66)	PP_5673/flhB	D‑alanine‑‑D‑alanine ligase/flagellin export apparatus substrate specificity protein

GCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGCTTGCCCTTGCCGGCGCGG  >  NC_002947/4945224‑4945516                                                                                                                                                     |                                                                                                                                                  gctcCTACATTTGTTTAGGGCCAGTTATTCATGCTGGATTTACGCGCGAACGCCTTCGCGTACAACTAGATATTGCGCCGTACAAACAAGGCGGTCGCGAGCGCCGGGCACAGGAGGGACTGGCACGAAACAAATGTAGGAGCGAGCtt                                                                                                                                                   >  8:235073/1‑149 (MQ=25)      tACATTTGTTTGGGGCATTTTTTTCGTGCGGTTTTAACGCGCGACCGCCTGCGCTACCCCCTCCATATTGCGCTGTACAAACACGGCGGTGGGGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTcg                                                                                                                                              <  4:81032/149‑1 (MQ=21)             gTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATgcg                                                                                                                                       >  8:277619/1‑149 (MQ=255)              tttCGGGCCATTAATTCCGCCGGGATTTACGCGCGACCCCCTTCGCTTACACCTCGATATTGCCCCGTACAAACATGGCGGGCGGGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATgcg                                                                                                                                       <  4:87411/148‑1 (MQ=37)                tCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCAATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGcgccg                                                                                                                                    >  1:98027/1‑149 (MQ=255)                  gggCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCcgcg                                                                                                                                  >  1:301326/1‑149 (MQ=255)                   ggCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCcgcgc                                                                                                                                 >  1:172580/1‑149 (MQ=255)                               cTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGTAAATCCCGCAGGAATAACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTc                                                                                                                     >  2:257326/1‑148 (MQ=17)                               cTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCcgcgc                                                                                                                                 >  1:349022/1‑137 (MQ=255)                               cTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTc                                                                                                                     >  3:374984/1‑148 (MQ=255)                                tGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGa                                                                                                                   >  5:34673/1‑149 (MQ=255)                                         tACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAg                                                                                                           >  3:285195/1‑148 (MQ=255)                                         accgcgcgACCCCCTTCGCGTACACCTCGATATTGCGCCTTACAACCATGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAg                                                                                                           <  4:285195/146‑1 (MQ=255)                                              gcgACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACg                                                                                                     >  7:224779/1‑149 (MQ=255)                                                   cGCCTTCGCTTACACCTCTATTTTGCGCCGTAAAAACACGGCGGTGGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCGACAAAAAc                                                                                                <  2:98027/149‑1 (MQ=18)                                                   cGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAAc                                                                                                >  1:154341/1‑149 (MQ=255)                                                      cTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGTCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTa                                                                                             >  2:287258/1‑149 (MQ=255)                                                                  cTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGaa                                                                                     >  5:391399/1‑145 (MQ=255)                                                                  cTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGa                                                                                 <  1:287258/149‑1 (MQ=255)                                                                        atTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTca                                                                                                              <  2:290864/114‑1 (MQ=255)                                                                        atTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTca                                                                                                              >  1:290864/1‑114 (MQ=255)                                                                         ttGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTg                                                                          >  6:7802/1‑149 (MQ=255)                                                                          tGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGt                                                                         >  3:68911/1‑149 (MQ=255)                                                                                  acaaacaaGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaaacctccggcaaacacctcaaaattccaacgccg                                                                 <  5:215920‑M2/149‑83 (MQ=255)                                                                                   caaacaaGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCgcggcg                                                                  >  4:91089/1‑147 (MQ=255)                                                                                    aaacaaGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCa                                                                                                   <  5:357056/113‑1 (MQ=255)                                                                                    aaacaaGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCa                                                                                                   >  6:357056/1‑113 (MQ=255)                                                                                            gggggccgcgcgcCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCcggcg                                                       <  6:34673/143‑1 (MQ=255)                                                                                             ggTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctcc                                                                                            >  2:262891‑M2/1‑56 (MQ=255)                                                                                             ggTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctcc                                                                                            <  1:262891‑M2/111‑56 (MQ=255)                                                                                               tCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaaacctccggcaaacacctcaaaattccaacgccgtccactcaaaatt                                                    >  6:215920‑M2/1‑54 (MQ=255)                                                                                                   gcgcgcCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCgg                                                >  5:389233/1‑149 (MQ=255)                                                                                                       gcCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAgg                                            <  8:224779/149‑1 (MQ=255)                                                                                                          gggCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGgtcg                                         <  2:154341/149‑1 (MQ=255)                                                                                                             tgcaggAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattccaacgccgccccc                                      >  6:323725‑M2/3‑40 (MQ=255)                                                                                                              acaGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTcg                                                                       >  2:53221/1‑115 (MQ=255)                                                                                                              acaGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTcg                                                                       >  2:58692/1‑115 (MQ=255)                                                                                                              acaGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTcg                                                                       <  1:53221/115‑1 (MQ=255)                                                                                                              acaGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTcg                                                                       <  1:58692/115‑1 (MQ=255)                                                                                                                 ggaggGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattccaacgccgccccctcaa                                  >  8:356525‑M2/1‑36 (MQ=255)                                                                                                                  gaggGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAg                                 >  3:103776/1‑149 (MQ=255)                                                                                                                         tGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGctct                          >  8:78713/1‑149 (MQ=255)                                                                                                                           gCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGctct                          <  7:78713/147‑1 (MQ=255)                                                                                                                             ccGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGgc                      >  4:245332/1‑149 (MQ=255)                                                                                                                             ccGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGgc                      >  4:217793/1‑149 (MQ=255)                                                                                                                              cGAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattccaacgccgccccctcaaaattccaacgcc                      <  5:347529‑M2/149‑126 (MQ=255)                                                                                                                                  ccaccggTACGAGCGAGCCGGCTCGCGATGCGCCGCGC‑GGGCGGCGCGCGAGCGCCCCGGCCCCGCAAACCTCTCCCCGCACCGCTCCACTGTCGCGGCGCAGGTCCGGCGGGAGCGGCAGGCCGCCCTTCAGCGGCTCTGGCCGcctg                 <  4:36706/143‑1 (MQ=255)                                                                                                                                   cAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGcttg                 >  3:36706/1‑148 (MQ=255)                                                                                                                                    aaaTGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCTGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGcttgc                >  1:254000/1‑149 (MQ=255)                                                                                                                                    aaaTGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGcttgcc               >  7:145266/1‑149 (MQ=255)                                                                                                                                      aTGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGcttgccct             >  6:307413/1‑149 (MQ=255)                                                                                                                                        gTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGcttgcccttg           >  2:345971/1‑149 (MQ=255)                                                                                                                                         tAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGcttgcccttgc          >  1:177657/1‑149 (MQ=255)                                                                                                                                         tAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGcttgcccttg           <  7:277619/148‑1 (MQ=255)                                                                                                                                           ggAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattccaacgccgccccctcaaaattccaacgccgcctcccctcga          >  5:153984‑M2/1‑11 (MQ=255)                                                                                                                                               cgagcTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGCTTGCCCTTGCCGgcgc    <  4:103776/149‑1 (MQ=255)                                                                                                                                                gagcTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGCTTGCCCATGCCGGcgcg   >  5:104219/1‑149 (MQ=255)                                                                                                                                                  gcTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGCTTGCCCTTGCCGGCGCgg  >  2:356181/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGC‑GGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGCTTGCCCTTGCCGGCGCGG  >  NC_002947/4945224‑4945516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.