Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,300,855 A→T 6.1% S18T (TCC→ACC) ‡ lpxH ← UDP‑2,3‑diacylglucosamine hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,300,8550AT 6.1% 245.0 / 3.4 81S18T (TCC→ACC) ‡lpxHUDP‑2,3‑diacylglucosamine hydrolase
Reads supporting (aligned to +/- strand):  ref base A (36/40);  new base T (2/3);  total (38/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

GCCGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTCGAC  >  NC_002947/3300714‑3301001
                                                                                                                                             |                                                                                                                                                   
gCCGGCGCATGGCCCGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATcc                                                                                                                                              <  8:169264/149‑1 (MQ=255)
  cGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCgg                                                                                                                                            <  5:41428/149‑1 (MQ=255)
  cGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCgg                                                                                                                                            <  2:63043/149‑1 (MQ=255)
    gcgcATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGc                                                                                                                                          <  3:354629/149‑1 (MQ=255)
        aTGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGttc                                                                                                                                      >  3:288573/1‑149 (MQ=255)
         tGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGATATTTCCCGGGCGtttc                                                                                                                                     <  4:376775/149‑3 (MQ=255)
         tGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGATATTTCCCGGGCGtttc                                                                                                                                     <  4:253437/149‑3 (MQ=255)
           gCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGttct‑tc                                                                                                                                  <  7:44145/149‑1 (MQ=255)
              tGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTAGAAGAAGTCCCCAAGGATGTACAGGGCCATGGCATGGCGGGCGCGTCAATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTg                                                                                                                               >  7:284966/1‑149 (MQ=255)
                gCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCa                                                                                                                             <  5:153350/149‑1 (MQ=255)
                gCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCa                                                                                                                             <  2:259882/149‑1 (MQ=255)
                gCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCa                                                                                                                             <  2:146991/149‑1 (MQ=255)
                          tgctgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCagat                                                                                                                    >  7:244409/1‑148 (MQ=255)
                            ctgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCagatcag                                                                                                                 <  6:294034/149‑1 (MQ=255)
                            ctgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCagatca                                                                                                                  <  8:244409/148‑1 (MQ=255)
                            ctgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCagat                                                                                                                    >  6:381220/1‑146 (MQ=255)
                             tgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCGCCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCa                                                                                                                       >  8:186636/1‑142 (MQ=255)
                             tgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCGCCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCa                                                                                                                       <  7:186636/142‑1 (MQ=255)
                             tgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCagatca                                                                                                                  >  8:45619/1‑147 (MQ=255)
                             tgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCagatca                                                                                                                  >  5:296682/1‑147 (MQ=255)
                                  tggCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCagatcagagatca                                                                                                           <  5:53038/148‑1 (MQ=255)
                                  gggCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACATGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCagatcagagatca                                                                                                           <  5:381220/149‑1 (MQ=255)
                                  gggCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCagatcagagatca                                                                                                           <  1:332221/149‑1 (MQ=255)
                                               tcgtcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGCGCCTTGGCATGGCGGGCGCGTCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCa                                                                                              <  8:284966/149‑1 (MQ=255)
                                               tcgtcgCCAATCCACCCTTCGAAGAAGTCACCAAGGAAGTAAAGCGCCTTGGCATGGCGGGCGCGGCCAACGAGAAGATCAAGAAACTCCCGGGAAATATCAGGGCGTTCT‑TCTTGAAGGTGCAGATCAGAGATCAGCAGAATCACTc                                                                                               >  2:296882/1‑148 (MQ=255)
                                                    gCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATc                                                                                                                                               <  6:278077/96‑1 (MQ=255)
                                                    gCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATc                                                                                                                                               >  5:278077/1‑96 (MQ=255)
                                                         tCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCgg                                                                                    <  4:288573/149‑1 (MQ=255)
                                                         tCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCgg                                                                                    <  2:272769/149‑1 (MQ=255)
                                                               cTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTtct                                                                              <  7:45619/149‑1 (MQ=255)
                                                                ttCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCTTGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTtctc                                                                             >  2:84671/1‑149 (MQ=255)
                                                                ttCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTtctc                                                                             >  5:334732/1‑149 (MQ=255)
                                                                     agaagTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCgatga                                                                        >  6:26265/1‑149 (MQ=255)
                                                                      gaagTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGttct‑tc                                                                                                                                  <  4:33803/90‑1 (MQ=255)
                                                                      gaagTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGttct‑tc                                                                                                                                  >  3:33803/1‑90 (MQ=255)
                                                                       aagTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCgatgat                                                                       >  2:279131/1‑148 (MQ=255)
                                                                            actaaGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATTTCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCgatg                                                                         <  6:23391/138‑1 (MQ=255)
                                                                            actaaGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATTTCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCgatg                                                                         >  5:23391/4‑141 (MQ=255)
                                                                            aCCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcgtc                                                                 >  6:328516/1‑149 (MQ=255)
                                                                             ccAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcgtcg                                                                >  5:141043/1‑149 (MQ=255)
                                                                             ccAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcgtcg                                                                >  5:48614/1‑149 (MQ=255)
                                                                              cAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcgtcgt                                                               >  1:161002/1‑149 (MQ=255)
                                                                               aaGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACgtcgtc                                                              <  6:296682/149‑1 (MQ=255)
                                                                                  gATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATATCGGCTTTCTGGATGATCACGTCGTCAtt                                                           >  7:163917/1‑149 (MQ=255)
                                                                                       aCAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTtct‑tct                                                                                                                                 <  5:372101/74‑1 (MQ=255)
                                                                                       aCAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTtct‑tct                                                                                                                                 >  6:372101/1‑74 (MQ=255)
                                                                                       aCAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTa                                                      >  8:220042/1‑149 (MQ=255)
                                                                                        cAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTAc                                                     >  6:275528/1‑149 (MQ=255)
                                                                                              cTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCtg                                               >  8:344806/1‑149 (MQ=255)
                                                                                                 ggCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATACGGGAGTTCT‑TATTGCAGGTGCAGATCAGATATCAGCAGAATCAATCAATTATCTCGGCTTTCTCGATGATCCCGTCGACCTTCGGTACGAACtggtg                                            >  4:328986/1‑149 (MQ=255)
                                                                                                  gCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATcagca                                                                                                        <  1:235037/88‑1 (MQ=255)
                                                                                                  gCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATcagca                                                                                                        >  2:235037/1‑88 (MQ=255)
                                                                                                    aTGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAAt                                                                                            >  3:367197/1‑98 (MQ=255)
                                                                                                    aTGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAAt                                                                                            <  4:367197/98‑1 (MQ=255)
                                                                                                    aTGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGc                                          >  2:179855/1‑148 (MQ=255)
                                                                                                    aTGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGc                                          >  5:386979/1‑148 (MQ=255)
                                                                                                     tGGCGGGCGCGGCCATCTAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGc                                          >  1:112203/1‑147 (MQ=255)
                                                                                                       gcgggcgCGTCCATCTATCATATCAATAAACGCGCGTGAAATATCCGGGCGTTCTCTCTTCCTTGTCCACNTCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCa                                        <  1:122755/148‑1 (MQ=255)
                                                                                                          ggcgCGGCCATCTAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCttt                                   <  2:112203/149‑1 (MQ=255)
                                                                                                          ggcgCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCttt                                   <  7:344806/149‑1 (MQ=255)
                                                                                                                 ccATCGAGAAGATCAAGAAACGACCGGGAAATATCCGGGCGTTAT‑TCTTGCAGGTGCAGATCTGTGATCAGCAGAATCACTCAATGATCTCTGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAAc                              <  3:328986/147‑1 (MQ=255)
                                                                                                                   aTCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGt                          <  1:341093/149‑1 (MQ=255)
                                                                                                                    tCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTg                         <  8:281672/149‑1 (MQ=255)
                                                                                                                    tCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTg                         <  1:279131/149‑1 (MQ=255)
                                                                                                                    tCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTg                         <  6:141043/149‑1 (MQ=255)
                                                                                                                    tCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCA‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTg                         <  5:328516/149‑1 (MQ=255)
                                                                                                                      gAGCAGATCAAGAAACGCCCGGGAAATATCGGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGc                       >  1:31064/1‑149 (MQ=255)
                                                                                                                         cAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcg                                                                   <  1:197511/102‑1 (MQ=255)
                                                                                                                         cAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcg                                                                   >  2:197511/1‑102 (MQ=255)
                                                                                                                           gATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACtt                  <  6:48614/149‑1 (MQ=255)
                                                                                                                              cAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACttttt               >  1:199335/1‑149 (MQ=255)
                                                                                                                              cAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACttttt               >  3:85207/1‑149 (MQ=255)
                                                                                                                               gaagaaCGCCCGGATATTTCCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTc              <  2:291489/145‑1 (MQ=255)
                                                                                                                               gaagaaCGCCCGGATATTTCCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTc              >  1:291489/5‑149 (MQ=255)
                                                                                                                                 gAAACGCCCGGTAAATATCCGGTCGTTCT‑TCTTTCAGGTTCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGa            <  8:163917/149‑1 (MQ=255)
                                                                                                                                 gAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcgtc                                                                 >  2:202253/1‑96 (MQ=255)
                                                                                                                                 agaaCGCCCGGATATTTCCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCg             >  8:73815/3‑148 (MQ=255)
                                                                                                                                   aaCGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGAt           <  2:161002/148‑1 (MQ=255)
                                                                                                                                      gCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCg       <  7:220042/149‑1 (MQ=255)
                                                                                                                                         cGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTAGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTc     >  5:53577/1‑148 (MQ=255)
                                                                                                                                           ggAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTCGAc  <  1:179855/149‑1 (MQ=255)
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GCCGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCT‑TCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTCGAC  >  NC_002947/3300714‑3301001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑