Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,344,839 T→C 8.6% D97G (GAC→GGC)  PP_2941 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,344,8390TC 8.6% 195.4 / 7.3 71D97G (GAC→GGC) PP_2941hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (33/31);  new base C (3/3);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

ATACCAGCCGTCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCTCGG  >  NC_002947/3344697‑3344985
                                                                                                                                              |                                                                                                                                                  
aTACCAGCCGTCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTccgccc                                                                                                                                              >  4:44162/1‑149 (MQ=255)
 tACCAGCCGTCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTccgcccg                                                                                                                                             >  8:105633/1‑149 (MQ=255)
   ccAGCCGTCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCcgcg                                                                                                                                           >  4:319219/1‑149 (MQ=255)
          tCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGc                                                                                                                                    <  7:105633/149‑1 (MQ=255)
                  cGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCa                                                                                                                            >  1:47616/1‑149 (MQ=255)
                   gTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCGTGGCGTTGTTCGGCGCGGCACGGTCGGTCTCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGGCCTCGGGCCTGCTTCATCCAc                                                                                                                           <  4:359212/149‑1 (MQ=255)
                      aTGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCGTCCAccc                                                                                                                         <  5:298323/148‑1 (MQ=255)
                        gCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGc                                                                                                                                        <  7:150472/131‑1 (MQ=255)
                        gCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGc                                                                                                                                        >  8:150472/1‑131 (MQ=255)
                        gCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTggt                                                                                                                                   >  8:252358/1‑134 (MQ=255)
                        gCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTggt                                                                                                                                   <  7:252358/136‑3 (MQ=255)
                         ccTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGc                                                                                                                       >  5:199132/1‑147 (MQ=255)
                         ccTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCaa                                                                                                                     <  3:247561/149‑1 (MQ=255)
                         ccTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTggtga                                                                                                                                 >  1:278417/1‑133 (MQ=255)
                         ccTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTggtga                                                                                                                                 <  2:278417/137‑5 (MQ=255)
                          cTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAag                                                                                                                    >  5:259444/1‑149 (MQ=255)
                                      aGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGc                                                                                                        <  3:319219/149‑1 (MQ=255)
                                       gATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCg                                                                                                       <  3:44162/149‑1 (MQ=255)
                                        aTCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGt                                                                                                      >  5:382401/1‑149 (MQ=255)
                                           gCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCGGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTgg                                                                                                    <  2:136926/148‑1 (MQ=255)
                                           gCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGt                                                                                                   >  7:11557/1‑149 (MQ=255)
                                           gCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGt                                                                                                   <  2:47616/149‑1 (MQ=255)
                                            cccGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCAc                                                                                                                           >  4:153013/1‑124 (MQ=255)
                                            cccGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCAc                                                                                                                           <  3:153013/124‑1 (MQ=255)
                                            cccGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTc                                                                                                  <  8:223775/149‑1 (MQ=255)
                                             ccGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTg                                                                                                                                     <  2:150279/113‑1 (MQ=255)
                                             ccGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTg                                                                                                                                     >  1:150279/1‑113 (MQ=39)
                                                  agaagGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGgcgc                                                                                             >  6:211878/1‑148 (MQ=255)
                                                  agaagGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGgcg                                                                                              <  5:137166/147‑1 (MQ=255)
                                                  agaagGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGgcg                                                                                              >  6:137166/1‑147 (MQ=255)
                                                      ggggCCGTGTGCCAACTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCTCCAAATGCGGTCGCAAGCCCCATCTCACCAGGACCGCCCGCGGGCCTGCTGCATCCACCCGCAAGACTGCCACCCGCGTGGTCGGCGCGACGt                                                                                        >  8:311638/1‑149 (MQ=255)
                                                       gggCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGCCGtt                                                                                       >  8:76427/1‑149 (MQ=255)
                                                       gggCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGtt                                                                                       <  5:164137/149‑1 (MQ=255)
                                                        ggCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTg                                                                                      <  6:259444/149‑1 (MQ=255)
                                                          ccGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTa                                                                                    >  6:266415/1‑149 (MQ=255)
                                                          ccGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTa                                                                                    >  6:157300/1‑149 (MQ=255)
                                                                gCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGc                                                                               >  1:241147/1‑148 (MQ=255)
                                                                  cACCTTTTCGACCCTGGGGTTGATCGGCGCGGAACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCaa                                                                            <  4:326369/149‑1 (MQ=255)
                                                                  cACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGcc                                                                              >  6:22244/1‑147 (MQ=255)
                                                                  cACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGcc                                                                              >  2:297986/1‑147 (MQ=255)
                                                                    ccTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGt                                                                          >  6:145800/1‑149 (MQ=255)
                                                                           gACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGt                                                                                                                 <  5:225558/103‑1 (MQ=255)
                                                                           gACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGt                                                                                                                 >  6:225558/1‑103 (MQ=255)
                                                                           gACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTgg                                                                    <  5:211878/148‑1 (MQ=255)
                                                                                tGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCAGCAAGAGTGCAACCGGCGTGGTCGGCGCGCCGTTGTACCGGCCAAGTGGCTGGTCGCCg                                                              >  3:217221/1‑149 (MQ=255)
                                                                                    gTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACgt                                                          >  4:239487/1‑149 (MQ=255)
                                                                                        aTCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACgttgtt                                                      <  5:266415/149‑1 (MQ=255)
                                                                                         tCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTc                                                     >  4:95661/1‑149 (MQ=255)
                                                                                            gcgcGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCCCCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGc                                                  >  5:142387/1‑149 (MQ=255)
                                                                                            gcgcGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGc                                                  >  5:365350/1‑149 (MQ=255)
                                                                                            gcgcGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGc                                                  >  6:26941/1‑149 (MQ=255)
                                                                                                   aCGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCct                                           <  6:365350/149‑1 (MQ=255)
                                                                                                      gtcggtcgCCCAATGCGGTCGCCAGCCCCATCTCACGAGGTCCGCCCGCGGGCCTGCTTCATCGACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCTAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTg                                        <  7:311638/149‑1 (MQ=255)
                                                                                                      gtcggtcgCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTg                                                                                                                >  6:11379/1‑77 (MQ=255)
                                                                                                      gtcggtcgCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTg                                                                                                                <  5:11379/77‑1 (MQ=255)
                                                                                                                      ggTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTc                        <  2:191652/149‑1 (MQ=255)
                                                                                                                      ggTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTc                        <  5:22244/149‑1 (MQ=255)
                                                                                                                       gTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCg                       <  5:157300/149‑1 (MQ=255)
                                                                                                                        tCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGa                                                                                           >  6:647/1‑80 (MQ=255)
                                                                                                                        tCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGa                                                                                           <  5:647/80‑1 (MQ=255)
                                                                                                                          gCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTgg                                                                        <  6:19244/97‑1 (MQ=255)
                                                                                                                          gCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTgg                                                                        >  5:19244/1‑97 (MQ=255)
                                                                                                                                 ccATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCgt             <  6:369633/149‑1 (MQ=255)
                                                                                                                                 ccATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCgt             <  8:11557/149‑1 (MQ=255)
                                                                                                                                      tcACCAGGTCCGCCCGCGGGCCTTCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGc                                                                       <  3:166453/86‑1 (MQ=255)
                                                                                                                                      tcACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGc                                                                       >  4:166453/1‑86 (MQ=255)
                                                                                                                                      tcACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATg        >  3:112579/1‑149 (MQ=255)
                                                                                                                                        aCCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGcc      <  2:241147/149‑1 (MQ=255)
                                                                                                                                         ccAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTg                                                                     <  4:77152/85‑1 (MQ=255)
                                                                                                                                         ccAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTg                                                                     >  3:77152/1‑85 (MQ=255)
                                                                                                                                             gTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCTCgg  >  5:320010/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
ATACCAGCCGTCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCTCGG  >  NC_002947/3344697‑3344985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑