Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,344,850 G→A 8.8% G93G (GGC→GGT)  PP_2941 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,344,8500GA 8.8% 194.0 / 6.4 68G93G (GGC→GGT) PP_2941hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (31/31);  new base A (3/3);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

TCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCTCGGTACTGACTCAAC  >  NC_002947/3344707‑3344997
                                                                                                                                               |                                                                                                                                                   
tCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGc                                                                                                                                                <  7:105633/149‑1 (MQ=255)
        cGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCa                                                                                                                                        >  1:47616/1‑149 (MQ=255)
         gTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCGTGGCGTTGTTCGGCGCGGCACGGTCGGTCTCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGGCCTCGGGCCTGCTTCATCCAc                                                                                                                                       <  4:359212/149‑1 (MQ=255)
            aTGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCGTCCAccc                                                                                                                                     <  5:298323/148‑1 (MQ=255)
              gCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGc                                                                                                                                                    <  7:150472/131‑1 (MQ=255)
              gCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGc                                                                                                                                                    >  8:150472/1‑131 (MQ=255)
              gCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTggt                                                                                                                                               >  8:252358/1‑134 (MQ=255)
              gCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTggt                                                                                                                                               <  7:252358/136‑3 (MQ=255)
               ccTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGc                                                                                                                                   >  5:199132/1‑147 (MQ=255)
               ccTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCaa                                                                                                                                 <  3:247561/149‑1 (MQ=255)
               ccTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTggtga                                                                                                                                             >  1:278417/1‑133 (MQ=255)
               ccTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTggtga                                                                                                                                             <  2:278417/137‑5 (MQ=255)
                cTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAag                                                                                                                                >  5:259444/1‑149 (MQ=255)
                            aGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGc                                                                                                                    <  3:319219/149‑1 (MQ=255)
                             gATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCg                                                                                                                   <  3:44162/149‑1 (MQ=255)
                              aTCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGt                                                                                                                  >  5:382401/1‑149 (MQ=255)
                                 gCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCGGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTgg                                                                                                                <  2:136926/148‑1 (MQ=255)
                                 gCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGt                                                                                                               <  2:47616/149‑1 (MQ=255)
                                 gCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGt                                                                                                               >  7:11557/1‑149 (MQ=255)
                                  cccGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCAc                                                                                                                                       <  3:153013/124‑1 (MQ=255)
                                  cccGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCAc                                                                                                                                       >  4:153013/1‑124 (MQ=255)
                                  cccGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTc                                                                                                              <  8:223775/149‑1 (MQ=255)
                                   ccGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTg                                                                                                                                                 >  1:150279/1‑113 (MQ=39)
                                   ccGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTg                                                                                                                                                 <  2:150279/113‑1 (MQ=255)
                                        agaagGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGgcgc                                                                                                         >  6:211878/1‑148 (MQ=255)
                                        agaagGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGgcg                                                                                                          >  6:137166/1‑147 (MQ=255)
                                        agaagGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGgcg                                                                                                          <  5:137166/147‑1 (MQ=255)
                                            ggggCCGTGTGCCAACTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCTCCAAATGCGGTCGCAAGCCCCATCTCACCAGGACCGCCCGCGGGCCTGCTGCATCCACCCGCAAGACTGCCACCCGCGTGGTCGGCGCGACGt                                                                                                    >  8:311638/1‑149 (MQ=255)
                                             gggCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGCCGtt                                                                                                   >  8:76427/1‑149 (MQ=255)
                                             gggCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGtt                                                                                                   <  5:164137/149‑1 (MQ=255)
                                              ggCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTg                                                                                                  <  6:259444/149‑1 (MQ=255)
                                                ccGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTa                                                                                                >  6:157300/1‑149 (MQ=255)
                                                ccGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTa                                                                                                >  6:266415/1‑149 (MQ=255)
                                                      gCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGc                                                                                           >  1:241147/1‑148 (MQ=255)
                                                        cACCTTTTCGACCCTGGGGTTGATCGGCGCGGAACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCaa                                                                                        <  4:326369/149‑1 (MQ=255)
                                                        cACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGcc                                                                                          >  2:297986/1‑147 (MQ=255)
                                                        cACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGcc                                                                                          >  6:22244/1‑147 (MQ=255)
                                                          ccTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGt                                                                                      >  6:145800/1‑149 (MQ=255)
                                                                 gACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGt                                                                                                                             >  6:225558/1‑103 (MQ=255)
                                                                 gACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGt                                                                                                                             <  5:225558/103‑1 (MQ=255)
                                                                 gACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTgg                                                                                <  5:211878/148‑1 (MQ=255)
                                                                      tGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCAGCAAGAGTGCAACCGGCGTGGTCGGCGCGCCGTTGTACCGGCCAAGTGGCTGGTCGCCg                                                                          >  3:217221/1‑149 (MQ=255)
                                                                          gTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACgt                                                                      >  4:239487/1‑149 (MQ=255)
                                                                              aTCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACgttgtt                                                                  <  5:266415/149‑1 (MQ=255)
                                                                               tCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTc                                                                 >  4:95661/1‑149 (MQ=255)
                                                                                  gcgcGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCCCCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGc                                                              >  5:142387/1‑149 (MQ=255)
                                                                                  gcgcGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGc                                                              >  5:365350/1‑149 (MQ=255)
                                                                                  gcgcGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGc                                                              >  6:26941/1‑149 (MQ=255)
                                                                                         aCGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCct                                                       <  6:365350/149‑1 (MQ=255)
                                                                                            gtcggtcgCCCAATGCGGTCGCCAGCCCCATCTCACGAGGTCCGCCCGCGGGCCTGCTTCATCGACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCTAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTg                                                    <  7:311638/149‑1 (MQ=255)
                                                                                            gtcggtcgCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTg                                                                                                                            >  6:11379/1‑77 (MQ=255)
                                                                                            gtcggtcgCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTg                                                                                                                            <  5:11379/77‑1 (MQ=255)
                                                                                                            ggTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTc                                    <  2:191652/149‑1 (MQ=255)
                                                                                                            ggTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTc                                    <  5:22244/149‑1 (MQ=255)
                                                                                                             gTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCg                                   <  5:157300/149‑1 (MQ=255)
                                                                                                              tCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGa                                                                                                       <  5:647/80‑1 (MQ=255)
                                                                                                              tCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGa                                                                                                       >  6:647/1‑80 (MQ=255)
                                                                                                                gCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTgg                                                                                    <  6:19244/97‑1 (MQ=255)
                                                                                                                gCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTgg                                                                                    >  5:19244/1‑97 (MQ=255)
                                                                                                                       ccATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCgt                         <  6:369633/149‑1 (MQ=255)
                                                                                                                       ccATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCgt                         <  8:11557/149‑1 (MQ=255)
                                                                                                                            tcACCAGGTCCGCCCGCGGGCCTTCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGc                                                                                   <  3:166453/86‑1 (MQ=255)
                                                                                                                            tcACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGc                                                                                   >  4:166453/1‑86 (MQ=255)
                                                                                                                            tcACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATg                    >  3:112579/1‑149 (MQ=255)
                                                                                                                              aCCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGcc                  <  2:241147/149‑1 (MQ=255)
                                                                                                                               ccAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTg                                                                                 >  3:77152/1‑85 (MQ=255)
                                                                                                                               ccAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTg                                                                                 <  4:77152/85‑1 (MQ=255)
                                                                                                                                   gTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCTCgg              >  5:320010/1‑148 (MQ=255)
                                                                                                                                             gggCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCTCGGTACTGACTCaa   >  4:317522/1‑149 (MQ=255)
                                                                                                                                              ggCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCTCGGTACTGACTCAAc  <  3:95661/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
TCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCTCGGTACTGACTCAAC  >  NC_002947/3344707‑3344997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑