Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,356,508 A→T 6.4% F114I (TTT→ATT) ‡ PP_2952 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,356,5080AT 6.4% 182.9 / 2.8 62F114I (TTT→ATT) ‡PP_2952LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (27/31);  new base T (2/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

GTTCCAAGGTGCCAGTGGCAGGGCTATATAGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATCAGGGCCGAGGGCAGGTAC  >  NC_002947/3356362‑3356656
                                                                                                                                                  |                                                                                                                                                    
gTTCCAAGGTGCCAGTGGCAGGGCTATATAGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACg                                                                                                                                                    >  5:45382/1‑149 (MQ=255)
gTTCCAAGGTGCCAGTGGCAGGGCTATATAGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACg                                                                                                                                                    >  4:56773/1‑149 (MQ=255)
   ccAAGGTGCCAGTGGCAGGGCTATATAGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGcc                                                                                                                                                  <  2:147017/148‑1 (MQ=255)
    cAAGGTGCCAGTGGCAGGGCTATATAGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGt                                                                                                                                                <  8:250974/149‑1 (MQ=255)
      aGGTGCCAGTGGCAGGGCTATATAGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGa                                                                                                                                              <  3:56773/149‑1 (MQ=255)
              gTGGCAGGGCTATATAGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAggg                                                                                                                                       >  2:318615/1‑148 (MQ=255)
                   aGGGCTATATAGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGgcagcag                                                                                                                                 >  5:7275/1‑149 (MQ=255)
                   aGGGCTATATAGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGgcagcag                                                                                                                                 >  5:175759/1‑149 (MQ=255)
                   aGGGCTATATAGTTGGCGTCGGCGTTCAGGCAATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGgcagcag                                                                                                                                 >  1:230550/1‑149 (MQ=255)
                          tataGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTg                                                                                                                          >  1:7378/1‑149 (MQ=255)
                          tataGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTg                                                                                                                          <  4:373442/149‑1 (MQ=255)
                              gTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTgcgc                                                                                                                       <  2:103004/148‑1 (MQ=255)
                                 ggCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGcccc                                                                                                                    <  2:271272/148‑1 (MQ=255)
                                  gCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGccc                                                                                                                     >  1:249333/1‑146 (MQ=255)
                                        gCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGa                                                                                                            <  1:44114/149‑1 (MQ=255)
                                               ggCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGcgcg                                                                                                     <  1:190814/149‑1 (MQ=255)
                                                gCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGccc                                                                                                                     >  1:94469/1‑132 (MQ=255)
                                                gCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGccc                                                                                                                     <  2:94469/132‑1 (MQ=255)
                                                  cATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGcc                                                                                                  >  1:317871/1‑149 (MQ=255)
                                                  cATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGc                                                                                                   >  1:35527/1‑148 (MQ=255)
                                                    tAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCAc                                                                                                <  3:285277/149‑1 (MQ=255)
                                                          aTGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCaa                                                                                          <  2:249333/149‑1 (MQ=255)
                                                                atatCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTcc                                                                                    <  2:230550/149‑1 (MQ=255)
                                                                   tCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCt                                                                                 >  5:326948/1‑149 (MQ=255)
                                                                     tACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGtt                                                                                                                           <  4:301902/105‑1 (MQ=255)
                                                                     tACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGtt                                                                                                                           >  3:301902/1‑105 (MQ=255)
                                                                     tACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCt                                                                                 >  4:217406/1‑147 (MQ=255)
                                                                       ccGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCt                                                                                 <  2:317871/145‑1 (MQ=255)
                                                                       ccGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCt                                                                                 <  1:318615/145‑1 (MQ=255)
                                                                       ccGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAg                                                                              <  2:352052/148‑1 (MQ=255)
                                                                          gATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTgg                                                                          >  1:295775/1‑149 (MQ=255)
                                                                             cgcgAAAAAGATCGCCGATCTGGTCGGAGAGAAACAAGCGCACGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGCAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGgcg                                                                       >  3:224375/1‑149 (MQ=255)
                                                                                  aaaaGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTc                                                                  <  2:35174/149‑1 (MQ=255)
                                                                                  aaaaGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTc                                                                  >  1:35174/1‑149 (MQ=255)
                                                                                       tttcgCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCt                                                                <  5:234778/142‑1 (MQ=255)
                                                                                        tCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACg                                                            <  2:185772/149‑1 (MQ=255)
                                                                                        tCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACg                                                            >  8:204391/1‑149 (MQ=255)
                                                                                         cGGCGATCTGGTCGGAGAGAAACAAGCGCTGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACg                                                            >  6:89685/1‑148 (MQ=255)
                                                                                            cGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAgg                                                        <  2:7378/149‑1 (MQ=255)
                                                                                                 tGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTc                                                   >  4:340350/1‑149 (MQ=255)
                                                                                                   gTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTgg                                                                          >  4:202961/1‑124 (MQ=255)
                                                                                                   gTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTc                                                   <  8:278009/147‑1 (MQ=255)
                                                                                                   gTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTc                                                   <  2:367749/147‑1 (MQ=255)
                                                                                                     cGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCgcgc                                               >  4:16348/1‑149 (MQ=255)
                                                                                                      ggAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGcc                                              >  4:156303/1‑149 (MQ=255)
                                                                                                           gaAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGc                                         <  6:45382/149‑1 (MQ=255)
                                                                                                             caCAAGCGCAGGTTCAACTGTGGTTGCTGTCGGCGAAACGCCGTGAGCCAGGGGAGCTGCACGTTGCGCCCCATATCGGAGGGCGCGGCGACCTGCAAGGTTCCGCTGAGCTGGGCGCTTTCGTGACGCAGGTTGTCGCGACCGTGCCt                                       <  4:224375/148‑1 (MQ=255)
                                                                                                              aCAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGTGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGc                                         <  6:122401/146‑1 (MQ=255)
                                                                                                              aCAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCt                                       >  3:42302/1‑148 (MQ=255)
                                                                                                              aCAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGAGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGc                                         >  5:122401/1‑146 (MQ=255)
                                                                                                                aaGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAg                                    <  8:153609/149‑1 (MQ=255)
                                                                                                                 aGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAgg                                   <  2:295775/149‑1 (MQ=255)
                                                                                                                        gTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCa                            <  7:204391/149‑1 (MQ=255)
                                                                                                                               tgtgGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGAt                     >  5:49203/1‑149 (MQ=255)
                                                                                                                                    aTGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATCAggg                <  6:326948/149‑1 (MQ=255)
                                                                                                                                      gCTCACGGCGTTTCGCCGAGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAgg                                   >  4:61497/1‑128 (MQ=255)
                                                                                                                                      gCTCACGGCGTTTCGCCGAGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAgg                                   <  3:61497/128‑1 (MQ=255)
                                                                                                                                      gCTCACGGCGTTTCGCCGAGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATc                    <  6:309445/143‑1 (MQ=255)
                                                                                                                                      gCTCACGGCGTTTCGCCGAGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATc                    >  5:309445/1‑143 (MQ=255)
                                                                                                                                        tctcGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATCAGGGcc              >  7:225572/1‑147 (MQ=255)
                                                                                                                                            ggCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATCAGGGCCGAgg          <  3:16348/147‑1 (MQ=255)
                                                                                                                                               gAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATCAGGGCCGAGGGCAgg     <  3:217406/149‑1 (MQ=255)
                                                                                                                                                  aCGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCCGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATCAGGGCCGAGGGCAGGTAc  <  8:344714/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GTTCCAAGGTGCCAGTGGCAGGGCTATATAGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATCAGGGCCGAGGGCAGGTAC  >  NC_002947/3356362‑3356656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑