Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,356,514 T→A 6.7% T112S (ACG→TCG)  PP_2952 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,356,5140TA 6.7% 173.1 / 3.3 60T112S (ACG→TCG) PP_2952LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (26/30);  new base A (2/2);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01

CAAGGTGCCAGTGGCAGGGCTATATAGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATCAGGGCCGAGGGCAGGTACTG  >  NC_002947/3356366‑3356658
                                                                                                                                                    |                                                                                                                                                
cAAGGTGCCAGTGGCAGGGCTATATAGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGt                                                                                                                                                  <  8:250974/149‑1 (MQ=255)
  aGGTGCCAGTGGCAGGGCTATATAGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGa                                                                                                                                                <  3:56773/149‑1 (MQ=255)
          gTGGCAGGGCTATATAGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAggg                                                                                                                                         >  2:318615/1‑148 (MQ=255)
               aGGGCTATATAGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGgcagcag                                                                                                                                   >  5:175759/1‑149 (MQ=255)
               aGGGCTATATAGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGgcagcag                                                                                                                                   >  5:7275/1‑149 (MQ=255)
               aGGGCTATATAGTTGGCGTCGGCGTTCAGGCAATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGgcagcag                                                                                                                                   >  1:230550/1‑149 (MQ=255)
                      tataGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTg                                                                                                                            <  4:373442/149‑1 (MQ=255)
                      tataGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTg                                                                                                                            >  1:7378/1‑149 (MQ=255)
                          gTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTgcgc                                                                                                                         <  2:103004/148‑1 (MQ=255)
                             ggCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGcccc                                                                                                                      <  2:271272/148‑1 (MQ=255)
                              gCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGccc                                                                                                                       >  1:249333/1‑146 (MQ=255)
                                    gCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGa                                                                                                              <  1:44114/149‑1 (MQ=255)
                                           ggCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGcgcg                                                                                                       <  1:190814/149‑1 (MQ=255)
                                            gCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGccc                                                                                                                       <  2:94469/132‑1 (MQ=255)
                                            gCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGccc                                                                                                                       >  1:94469/1‑132 (MQ=255)
                                              cATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGcc                                                                                                    >  1:317871/1‑149 (MQ=255)
                                              cATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGc                                                                                                     >  1:35527/1‑148 (MQ=255)
                                                tAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCAc                                                                                                  <  3:285277/149‑1 (MQ=255)
                                                      aTGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCaa                                                                                            <  2:249333/149‑1 (MQ=255)
                                                            atatCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTcc                                                                                      <  2:230550/149‑1 (MQ=255)
                                                               tCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCt                                                                                   >  5:326948/1‑149 (MQ=255)
                                                                 tACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGtt                                                                                                                             <  4:301902/105‑1 (MQ=255)
                                                                 tACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGtt                                                                                                                             >  3:301902/1‑105 (MQ=255)
                                                                 tACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCt                                                                                   >  4:217406/1‑147 (MQ=255)
                                                                   ccGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCt                                                                                   <  1:318615/145‑1 (MQ=255)
                                                                   ccGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCt                                                                                   <  2:317871/145‑1 (MQ=255)
                                                                   ccGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAg                                                                                <  2:352052/148‑1 (MQ=255)
                                                                      gATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTgg                                                                            >  1:295775/1‑149 (MQ=255)
                                                                         cgcgAAAAAGATCGCCGATCTGGTCGGAGAGAAACAAGCGCACGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGCAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGgcg                                                                         >  3:224375/1‑149 (MQ=255)
                                                                              aaaaGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTc                                                                    <  2:35174/149‑1 (MQ=255)
                                                                              aaaaGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTc                                                                    >  1:35174/1‑149 (MQ=255)
                                                                                   tttcgCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCt                                                                  <  5:234778/142‑1 (MQ=255)
                                                                                    tCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACg                                                              >  8:204391/1‑149 (MQ=255)
                                                                                    tCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACg                                                              <  2:185772/149‑1 (MQ=255)
                                                                                     cGGCGATCTGGTCGGAGAGAAACAAGCGCTGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACg                                                              >  6:89685/1‑148 (MQ=255)
                                                                                        cGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAgg                                                          <  2:7378/149‑1 (MQ=255)
                                                                                             tGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTc                                                     >  4:340350/1‑149 (MQ=255)
                                                                                               gTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTgg                                                                            >  4:202961/1‑124 (MQ=255)
                                                                                               gTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTc                                                     <  8:278009/147‑1 (MQ=255)
                                                                                               gTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTc                                                     <  2:367749/147‑1 (MQ=255)
                                                                                                 cGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCgcgc                                                 >  4:16348/1‑149 (MQ=255)
                                                                                                  ggAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGcc                                                >  4:156303/1‑149 (MQ=255)
                                                                                                       gaAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGc                                           <  6:45382/149‑1 (MQ=255)
                                                                                                         caCAAGCGCAGGTTCAACTGTGGTTGCTGTCGGCGAAACGCCGTGAGCCAGGGGAGCTGCACGTTGCGCCCCATATCGGAGGGCGCGGCGACCTGCAAGGTTCCGCTGAGCTGGGCGCTTTCGTGACGCAGGTTGTCGCGACCGTGCCt                                         <  4:224375/148‑1 (MQ=255)
                                                                                                          aCAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGTGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGc                                           <  6:122401/146‑1 (MQ=255)
                                                                                                          aCAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCt                                         >  3:42302/1‑148 (MQ=255)
                                                                                                          aCAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGAGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGc                                           >  5:122401/1‑146 (MQ=255)
                                                                                                            aaGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAg                                      <  8:153609/149‑1 (MQ=255)
                                                                                                             aGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAgg                                     <  2:295775/149‑1 (MQ=255)
                                                                                                                    gTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCa                              <  7:204391/149‑1 (MQ=255)
                                                                                                                           tgtgGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGAt                       >  5:49203/1‑149 (MQ=255)
                                                                                                                                aTGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATCAggg                  <  6:326948/149‑1 (MQ=255)
                                                                                                                                  gCTCACGGCGTTTCGCCGAGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAgg                                     >  4:61497/1‑128 (MQ=255)
                                                                                                                                  gCTCACGGCGTTTCGCCGAGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAgg                                     <  3:61497/128‑1 (MQ=255)
                                                                                                                                  gCTCACGGCGTTTCGCCGAGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATc                      >  5:309445/1‑143 (MQ=255)
                                                                                                                                  gCTCACGGCGTTTCGCCGAGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATc                      <  6:309445/143‑1 (MQ=255)
                                                                                                                                    tctcGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATCAGGGcc                >  7:225572/1‑147 (MQ=255)
                                                                                                                                        ggCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATCAGGGCCGAgg            <  3:16348/147‑1 (MQ=255)
                                                                                                                                           gAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATCAGGGCCGAGGGCAgg       <  3:217406/149‑1 (MQ=255)
                                                                                                                                              aCGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCCGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATCAGGGCCGAGGGCAGGTAc    <  8:344714/149‑1 (MQ=255)
                                                                                                                                                gCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATCAGGGCCGAGGGCAGGTACTg  >  2:168598/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CAAGGTGCCAGTGGCAGGGCTATATAGTTGGCGTCGGCGTTCAGGCCATAGCGGATGGCGATATCTACCGGATCGCGAAAAAGATCGGCGATCTGGTCGGAGAGAAACAAGCGCAGGTTCAACTGTGGATGCTCTCGGCGAAACGCCGTGAGCCAGGGCAGCAGCACGTTGCGCCCCAGATCGGAGGGCGCGGCCACCTGCAACGTTCCGCTGAGCTGGGCGCTTTCCTGACGCAGGTTGTCGCGCCCGTGCCTGAGGGCTTCCAGGGCGATCAGGGCCGAGGGCAGGTACTG  >  NC_002947/3356366‑3356658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑