| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,402,511 | G→C | 13.5% | intergenic (+35/‑117) | PP_3010 → / → PP_3011 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,402,511 | 0 | G | C | 13.5% | 129.8 / 13.6 | 52 | intergenic (+35/‑117) | PP_3010/PP_3011 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (27/18); new base C (4/3); total (31/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.44e-01 | |||||||||||
GTAGAGTTGCGGGGCACGGGAAGCGAGCGCCACTT‑CA‑CGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTTGGATGGAGAGTCG > NC_002947/3402366‑3402648 | gTAGAGTTGCGGGGCACGGGAAGCGAGCGCCACTT‑CA‑CGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGagag < 8:191673/148‑1 (MQ=255)gTAGAGTTGCGGGGCACGGGAAGCGAGCGCCACTT‑CA‑CGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGc > 3:309927/1‑149 (MQ=255) gTTGCGGGGCACGGGAAGCGAGCGCCACTTCCT‑CGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCACCCCCCACACCAATAGCCCGCTCTCGACGGCGGGc > 1:39507/1‑149 (MQ=255) gTTGCGGGGCACGGGAAGCGAGCGCCACTT‑CAGCGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCAAACCAATAGCCCGCTCTCGACGGCGGGc > 1:45261/1‑149 (MQ=255) tGCGGGGCACGGGAAGCGAGCGCCACTT‑CA‑CGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCttt > 7:361684/1‑149 (MQ=255) cGGGGCACGGGAAGCGAGCGCCACTT‑CA‑CGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCTCTCGACGGCGGGCtattg < 2:45261/149‑2 (MQ=255) cGGGGCACGGGAAGCGAGCGCCACTT‑CA‑CGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCTCTCGACGGCGGGCtattg < 2:39507/149‑2 (MQ=255) cGGGGCACGGGAAGCGAGCGCCACTT‑CA‑CGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCttttt < 3:349558/149‑1 (MQ=255) ggCACGGGAAGCGAGCTCCACTT‑CA‑CGGCCTATCAACGTGATGCCTTGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTtgt < 5:232128/148‑1 (MQ=255) ggCACGGGAAGCGAGCGCCACTT‑CA‑CGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCACAACACCAATAGCCAGCCGTCGAGAGCGGGCTATTtgt > 6:232128/1‑148 (MQ=255) gggAAGCGAGCGCCACTT‑CA‑CGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGcc > 6:108562/1‑148 (MQ=255) cACTT‑CA‑CGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCgg < 3:212035/149‑1 (MQ=255) ggCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGtt > 7:289741/1‑148 (MQ=255) ccTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGACCGGCAACGTTCa > 3:261710/1‑148 (MQ=255) tCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGGGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCaaaaaa < 6:75965/149‑1 (MQ=255) aaCGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAg > 6:341425/1‑149 (MQ=255) aCGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAgg > 1:51989/1‑149 (MQ=255) tGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTtgtgt < 2:310428/112‑1 (MQ=255) tGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTtgtgt > 1:310428/1‑112 (MQ=255) gATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCgg > 6:92264/1‑149 (MQ=255) gTACGACCATTGCTGGTTGCTTGATGCTGCACTGATGTGTTCAATAGCGTAATCATCCATCCCCCACACCAATAGGCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGcc < 4:261710/149‑1 (MQ=255) ccATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCgg > 5:152909/1‑114 (MQ=255) ccATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCgg < 6:152909/114‑1 (MQ=255) cAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGa < 2:51989/149‑1 (MQ=255) gTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTGATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGag > 8:57914/1‑147 (MQ=255) gctgcACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTg > 4:110636/1‑135 (MQ=255) gctgcACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTg < 3:110636/135‑1 (MQ=255) ctgcACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGtt > 6:61052/1‑143 (MQ=255) ctgcACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGtt < 5:61052/143‑1 (MQ=255) tgcACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTg > 6:206283/1‑148 (MQ=255) cACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCAt > 2:234182/1‑149 (MQ=255) cACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCAt > 5:229762/1‑149 (MQ=255) aTGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGc < 8:289741/148‑1 (MQ=255) ttCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGccc > 4:224187/1‑149 (MQ=255) ttCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGccc > 7:133838/1‑149 (MQ=255) ttCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGccc > 8:27318/1‑149 (MQ=255) gTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTaa < 6:229762/149‑1 (MQ=255) gTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTaa > 4:48246/1‑149 (MQ=255) tcatcCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATAtt > 5:146274/1‑149 (MQ=255) ccccACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAg > 1:344947/1‑86 (MQ=255) ccccACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAg < 2:344947/86‑1 (MQ=255) cccACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTAttt < 7:27318/149‑1 (MQ=255) gcacacaAAAAGCCCGCTCTCGACGGCGGGCTATTGGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTtg > 2:357465/2‑149 (MQ=38) ccACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTtg > 3:97743/1‑149 (MQ=255) acCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTtggatg < 5:92264/149‑1 (MQ=255) caaaaaGCCCGCTCTCGACGGCGGGCTATTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGtt < 6:151686/132‑1 (MQ=39) caaaaaGCCCGCTCTCGACGGCGGGCTATTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGtt > 5:151686/6‑137 (MQ=39) cAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTtggatgga < 4:340759/149‑1 (MQ=255) cccGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTtg < 7:70471/137‑1 (MQ=255) cccGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTtg > 8:70471/1‑137 (MQ=255) cgccgTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTTGGATGGAGAGTCg > 8:49683/1‑147 (MQ=255) ccgTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTtggatg < 7:165822/137‑1 (MQ=255) ccgTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTtggatg > 8:165822/1‑137 (MQ=255) | GTAGAGTTGCGGGGCACGGGAAGCGAGCGCCACTT‑CA‑CGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTTGGATGGAGAGTCG > NC_002947/3402366‑3402648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |