Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,430,349 G→C 5.2% S189S (TCG→TCC)  PP_3045 → ATP‑dependent protease ClpP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,430,3490GC 5.2% 244.9 / 3.2 77S189S (TCG→TCC) PP_3045ATP‑dependent protease ClpP
Reads supporting (aligned to +/- strand):  ref base G (36/37);  new base C (2/2);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01

CCGAGGACCTTCGTCGGGTGGCGGACGTGCTTGATCAGGCCTTCGAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAGGCCAGTAGGGATGGGGT  >  NC_002947/3430210‑3430492
                                                                                                                                           |                                                                                                                                               
ccGAGGACCTTCGTCGGGTGGCGGACGTGCTTGATCAGGCCTTCGAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTg                                                                                                                                        <  6:129972/149‑1 (MQ=255)
ccGAGGACCTTCGTCGGGTGGCGGACGTGCTTGATCAGGCCTTCGAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTg                                                                                                                                        <  4:147123/149‑1 (MQ=255)
  gAGGACCTTCGTCGGGTGGCGGACGTGCTTGATCAGGCCTTCGAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCCGAGGCCGTgag                                                                                                                                      <  3:358611/149‑3 (MQ=255)
       ccTTCGTCGGGTGGCGGACGTGCTTGATCAGGCCTTCGAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTgg                                                                                                                                 <  6:116308/149‑1 (MQ=255)
        cTTCGTCGGGTGGCGGCCGTGCTTGATCAGGCCTTCGAGGCAATGATCGCAGACTATAAGGCCAAGACTCCGACCATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGCGGCCGTGGCGCTggg                                                                                                                                >  1:285631/1‑149 (MQ=255)
        cTTCGTCGGGTGGCGGACGTGCTTGATCAGGCCTTCGAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGATGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTggg                                                                                                                                >  7:329199/1‑149 (MQ=255)
          tcgtcgGGTGGCGGACGTGCTTGATCAGGCCTTCGAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGc                                                                                                                              >  5:214399/1‑149 (MQ=255)
                   ggCGGACGTGCTTGATCAGGCCTTCGAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATg                                                                                                                     <  7:179742/149‑1 (MQ=255)
                       gACGTGCTTGATCAGGCCTTCGAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGt                                                                                                                 >  3:233733/1‑149 (MQ=255)
                                  tCAGGCCTTCGAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGgtg                                                                                                      >  6:185329/1‑149 (MQ=255)
                                      gCCTTCGAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGc                                                                                                                              <  8:260788/121‑1 (MQ=255)
                                      gCCTTCGAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGc                                                                                                                              >  7:260788/1‑121 (MQ=255)
                                      gCCTTCGAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTc                                                                                                    >  5:301311/1‑147 (MQ=255)
                                        cTTCGAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAgg                                                                                                <  3:259901/149‑1 (MQ=255)
                                         ttCGAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGc                                                                                                                          >  5:92149/1‑122 (MQ=255)
                                         ttCGAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGc                                                                                                                          <  6:92149/122‑1 (MQ=255)
                                         ttCGAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGt                                                                                               >  6:201135/1‑149 (MQ=255)
                                            gAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGtt                                                                                              >  1:29300/1‑147 (MQ=255)
                                            gAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTc                                                                                             <  7:296204/148‑1 (MQ=255)
                                               gCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGc                                                                                         <  5:185329/149‑1 (MQ=255)
                                                 aaTGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGt                                                                                       <  2:47245/149‑1 (MQ=255)
                                                 aaTGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGt                                                                                       >  6:377285/1‑149 (MQ=255)
                                                  aTGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGAt                                                                                                                      >  2:222494/1‑117 (MQ=255)
                                                   tGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGc                                                                                     >  1:349410/1‑149 (MQ=255)
                                                   tGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGc                                                                                     >  2:16503/1‑149 (MQ=255)
                                                        gCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGAAGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTg                                                                                 <  2:177325/148‑1 (MQ=255)
                                                           gCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGc                                                                              >  2:161965/1‑148 (MQ=255)
                                                             cTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCtt                                                                                  <  4:223730/142‑1 (MQ=255)
                                                             cTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCtt                                                                                  >  3:223730/1‑142 (MQ=255)
                                                              tataAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAgg                                                                          >  7:79356/1‑149 (MQ=255)
                                                              tataAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAgg                                                                          >  5:95282/1‑149 (MQ=255)
                                                                   ggCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGgt                                                                                                       <  4:266997/115‑1 (MQ=255)
                                                                   ggCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGgt                                                                                                       >  3:266997/1‑115 (MQ=255)
                                                                   ggCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCgcgg                                                                     <  6:214399/149‑1 (MQ=255)
                                                                    gCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCgcggc                                                                    >  6:275608/1‑149 (MQ=255)
                                                                    gCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCgcggc                                                                    <  4:233733/149‑1 (MQ=255)
                                                                       aaGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTgg                                                                                                                                 >  8:247677/1‑85 (MQ=255)
                                                                       aaGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTgg                                                                                                                                 <  7:247677/85‑1 (MQ=255)
                                                                              cGAAGATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGATTGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCTGGCGTGCCTTGGGCAAGGCGCGGCGATGgcgc                                                            <  2:198972/147‑1 (MQ=255)
                                                                              cGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGgcgc                                                            <  2:216649/147‑1 (MQ=255)
                                                                                   aTCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCg                                                     >  3:130626/1‑149 (MQ=255)
                                                                                   aTCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCg                                                     <  2:349410/149‑1 (MQ=255)
                                                                                   aTCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCg                                                     <  2:29300/149‑1 (MQ=255)
                                                                                   aTCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCg                                                     <  5:275608/149‑1 (MQ=255)
                                                                                      gATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCa                                                   <  5:201135/148‑1 (MQ=255)
                                                                                       aTGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCaa                                                  >  3:50274/1‑148 (MQ=255)
                                                                                           cgcgGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATAccc                                             <  2:285631/149‑1 (MQ=255)
                                                                                                aGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCaa                                        <  8:79356/149‑1 (MQ=255)
                                                                                                 gCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGAGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAg                                       >  5:1738/1‑149 (MQ=255)
                                                                                                     agaCGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGccc                                    >  2:198422/1‑148 (MQ=255)
                                                                                                       aCGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTAt                                 <  5:377285/149‑1 (MQ=255)
                                                                                                        cGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTAtt                                >  5:183480/1‑149 (MQ=255)
                                                                                                        cGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTAtt                                <  6:95282/149‑1 (MQ=255)
                                                                                                             ggTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGAt                            >  4:371684/1‑148 (MQ=255)
                                                                                                                 aaTGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGc                        <  6:1738/148‑1 (MQ=255)
                                                                                                                   tGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTc                      >  8:12396/1‑148 (MQ=255)
                                                                                                                     ccGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCaa                    <  3:371684/148‑1 (MQ=255)
                                                                                                                     ccGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAg                   <  1:198422/149‑1 (MQ=255)
                                                                                                                      cGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCCGGCCTGGCTTGGGCAAGGCGCGGCGCTGGCGCGTTACCGCACTACCCCGCAAGCCCTATTGCATCAGCTCAAgg                  >  7:76000/1‑149 (MQ=255)
                                                                                                                       gAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGcgcg                                                                      <  3:204478/96‑1 (MQ=255)
                                                                                                                       gAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGcgcg                                                                      >  4:204478/1‑96 (MQ=255)
                                                                                                                       gAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAGGc                 <  1:16503/149‑1 (MQ=255)
                                                                                                                       gAAAGTTGGCTCACGGCCGCGGAGGCCGTGGCGCGGGGGCTGGCCGATGATGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAGGc                 <  8:152287/149‑1 (MQ=255)
                                                                                                                         aaGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAGGCCa               <  4:50274/149‑1 (MQ=255)
                                                                                                                            ttGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAg                   >  1:129074/1‑142 (MQ=255)
                                                                                                                            ttGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAg                   <  2:129074/142‑1 (MQ=255)
                                                                                                                            ttGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAGGCCAg              >  1:203363/1‑147 (MQ=255)
                                                                                                                              ggCTCACGGCCTCGGAGGCCGTGGCGCTCGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAGGCCAGTAgg          >  4:183114/1‑149 (MQ=255)
                                                                                                                                gccACGGCCTCCGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAGGCCAGTAGGGa        >  1:58994/3‑149 (MQ=255)
                                                                                                                                gccACGGCCTCCGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAGGCCAGTAGGGa        >  7:235786/3‑149 (MQ=255)
                                                                                                                                  cACGGCCTGGTAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTATTGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGc                        <  4:156872/131‑1 (MQ=255)
                                                                                                                                  cACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGc                        >  3:156872/1‑131 (MQ=255)
                                                                                                                                    cGGCCTCCGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAGGCCAGTAGGGATggg    <  8:235786/149‑1 (MQ=255)
                                                                                                                                     ggCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAGGCCAGTAGGGATgggg   >  7:98205/1‑149 (MQ=255)
                                                                                                                                      gCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAGGCCAGTAGGGATGGGGt  <  8:329199/149‑1 (MQ=255)
                                                                                                                                         tCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAGGCCAGt             >  1:60503/1‑135 (MQ=255)
                                                                                                                                         tCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAGGCCAGt             <  2:60503/135‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                               
CCGAGGACCTTCGTCGGGTGGCGGACGTGCTTGATCAGGCCTTCGAGGCAATGATCGCAGCCTATAAGGCCAAGGCTCCGAACATCGATGACGCGGAGCTGAGACGTCTGGTCAATGCCGAAAGTTGGCTCACGGCCTCGGAGGCCGTGGCGCTGGGGCTGGCCGATGAGGTCGGTAATGGTGTCAAGGTTCAGGCGTGCCTTGGGCAAGGCGCGGCGATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAGGCCAGTAGGGATGGGGT  >  NC_002947/3430210‑3430492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑