Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,436,195	T→A	7.5%	W44R (TGG→AGG)	gpI →	tail formation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,436,195	0	T	A	7.5%	238.7 / 5.0	79	W44R (TGG→AGG)	gpI	tail formation protein
Reads supporting (aligned to +/- strand):  ref base T (39/34);  new base A (2/4);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCGGGT  >  NC_002947/3436051‑3436340                                                                                                                                                  |                                                                                                                                                  gTCGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCctggct                                                                                                                                               >  3:11249/1‑149 (MQ=255)   cGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCTCGCCGAACTTCC‑TGCCctggctgg                                                                                                                                             >  3:68252/1‑149 (MQ=255)   cGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCctggc                                                                                                                                                >  5:136196/1‑146 (MQ=255)   cGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCctggc                                                                                                                                                <  6:136196/146‑1 (MQ=255)     aaGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGcc                                                                                                                                           >  6:107386/1‑149 (MQ=255)             gACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGACCTTCCATGCCCAGGCCAGCCAGGGCag                                                                                                                                    >  4:325258/1‑148 (MQ=255)               cttGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCAGGCCAGCCAGGGCagga                                                                                                                                  <  3:285557/146‑4 (MQ=255)                     ccTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCctg                                                                                                                                                  <  1:303544/126‑1 (MQ=255)                     ccTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCctg                                                                                                                                                  >  2:303544/1‑126 (MQ=255)                      cTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCctggct                                                                                                                                               <  1:218123/128‑1 (MQ=255)                      cTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCctggct                                                                                                                                               >  2:218123/1‑128 (MQ=255)                         ttACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGACCTTCCATGCCCAGGCCAGCCAGGGCA‑GGAAGTTCggcg                                                                                                                        <  3:325258/148‑4 (MQ=37)                         ttACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCC‑TCGACCTTCCATGCCCAGGCCAGCCAGGGCA‑GGAAGTTCggcgg                                                                                                                       <  4:145987/148‑5 (MQ=25)                           aCCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTAGCCTGGGCATGGAAGGTCGAGGGCt                                                                                                                     >  8:97939/1‑149 (MQ=255)                            ccGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCtggctggc                                                                                                                                            >  6:25708/1‑125 (MQ=255)                            ccGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCtggctggc                                                                                                                                            <  5:25708/125‑1 (MQ=255)                              gCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCtggctggc                                                                                                                                            >  3:147111/1‑123 (MQ=255)                              gCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCtggctggc                                                                                                                                            <  4:147111/123‑1 (MQ=255)                              gCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGaa                                                                                                                                >  2:38481/1‑135 (MQ=255)                              gCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGaa                                                                                                                                <  1:38481/135‑1 (MQ=255)                              gCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTgg                                                                                                                   >  6:205264/1‑148 (MQ=255)                                  aaCGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAggc                                                                                                              >  7:67227/1‑149 (MQ=255)                                    cGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAggcgg                                                                                                            <  1:193594/149‑1 (MQ=255)                                      ccACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCt                                                                                                          <  2:343139/149‑1 (MQ=255)                                       cACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCtt                                                                                                         <  2:222892/149‑1 (MQ=255)                                       cACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCt                                                                                                          >  4:155647/1‑148 (MQ=255)                                       cACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCt                                                                                                          >  1:222892/1‑148 (MQ=255)                                          ccAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCAGGCCAGCCAGGGCagg                                                                                                                                   >  5:187030/1‑118 (MQ=255)                                          ccAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCAGGCCAGCCAGGGCagg                                                                                                                                   <  6:187030/120‑3 (MQ=255)                                           cAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTacac                                                                                                     <  4:11249/149‑1 (MQ=255)                                                 gAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACcgacga                                                                                               <  3:155647/149‑1 (MQ=255)                                                    cgcgcCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCa                                                                                            <  4:358189/149‑1 (MQ=255)                                                      cgcCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAgc                                                                                          <  5:107386/149‑1 (MQ=255)                                                         ccTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGcgcc                                                                                       >  4:250582/1‑149 (MQ=255)                                                         ccTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGcgcc                                                                                       >  3:269738/1‑149 (MQ=255)                                                           tGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGcgccgc                                                                                     >  2:92193/1‑149 (MQ=255)                                                             gAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCgacgac                                                                                              <  1:287434/138‑1 (MQ=255)                                                             gAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCgacgac                                                                                              >  2:287434/1‑138 (MQ=255)                                                                  ggcCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGa                                                                              >  6:6902/1‑149 (MQ=255)                                                                           ccTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAg                                                                      <  4:29408/148‑1 (MQ=255)                                                                           ccTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAg                                                                      >  1:191063/1‑148 (MQ=255)                                                                           ccTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAg                                                                      >  3:29408/1‑148 (MQ=255)                                                                           ccTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAg                                                                      <  2:191063/148‑1 (MQ=255)                                                                            cTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGc                                                                    >  4:190071/1‑149 (MQ=255)                                                                            cTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCCGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGc                                                                    >  3:45825/1‑149 (MQ=255)                                                                             ttGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAgg                                                                                                               <  4:132784/105‑1 (MQ=255)                                                                             ttGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAgg                                                                                                               >  3:132784/1‑105 (MQ=255)                                                                                 ccacgccccGGATAATGTCCGGGGCGTAGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTc                                                               >  8:199863/7‑149 (MQ=255)                                                                                 ccTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTc                                                               <  5:6902/149‑1 (MQ=255)                                                                                     gACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTAGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCgg                                                            <  7:97939/148‑1 (MQ=255)                                                                                      ccccGGATAATGTCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAGGGTCGAGGGCTGGGAGGCGGCTTACACCGCCGACCAGCGCCGCGCGCTGATCCGCGTGGCCATTCCGGt                                                           >  8:167386/2‑148 (MQ=255)                                                                                      aCCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCTCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGtt                                                          <  4:68252/149‑1 (MQ=255)                                                                                       cccGGATAATGTCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGa                                                                       >  7:10186/1‑135 (MQ=255)                                                                                       cccGGATAATGTCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGa                                                                       <  8:10186/135‑1 (MQ=255)                                                                                        ccGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTc                                                                                                                            >  3:314306/1‑81 (MQ=255)                                                                                        ccGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTc                                                                                                                            <  4:314306/81‑1 (MQ=255)                                                                                         cGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTca                                                        >  2:223002/1‑148 (MQ=255)                                                                                           gACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTggg                                                                                                                                       >  5:12316/1‑67 (MQ=255)                                                                                           gACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTggg                                                                                                                                       <  6:12316/67‑1 (MQ=255)                                                                                              aTTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGAc                                                  <  5:205264/149‑1 (MQ=255)                                                                                                  tCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAg                                              >  6:210873/1‑149 (MQ=255)                                                                                                    cGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGa                                                                       <  2:333791/122‑1 (MQ=255)                                                                                                    cGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGa                                                                       >  1:333791/1‑122 (MQ=255)                                                                                                    cGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAgg                                             <  1:92193/148‑1 (MQ=255)                                                                                                     ggggCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGc                                           <  8:67227/149‑1 (MQ=255)                                                                                                     ggggCGTGGCCGACTCGTAACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCCGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGAACAAggg                                            >  8:309427/1‑148 (MQ=255)                                                                                                      gggCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGtt                                                          >  6:96141/1‑133 (MQ=255)                                                                                                      gggCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGtt                                                          <  5:96141/133‑1 (MQ=255)                                                                                                             gCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTc                                                               <  1:13040/121‑1 (MQ=255)                                                                                                             gCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTc                                                               >  2:13040/1‑121 (MQ=255)                                                                                                             gCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCgg                                   >  7:348416/1‑149 (MQ=255)                                                                                                              ccGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCa                                          >  8:91683/1‑141 (MQ=255)                                                                                                              ccGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCa                                          <  7:91683/141‑1 (MQ=255)                                                                                                                 aCTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGgcgc                                >  8:288666/1‑148 (MQ=255)                                                                                                                  cTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGa                              >  7:344086/1‑149 (MQ=255)                                                                                                                  cTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCAAAAGACCAAGGGCACGGTCGGCGCGa                              <  7:199863/149‑1 (MQ=255)                                                                                                                     gTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAg                                              <  1:74469/130‑1 (MQ=255)                                                                                                                     gTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAg                                              >  2:74469/1‑130 (MQ=255)                                                                                                                     gTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGAt                             <  7:288666/147‑1 (MQ=255)                                                                                                                     gTCACGCTGCCCGCCGAAATTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAAGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTc                           >  5:90638/1‑149 (MQ=255)                                                                                                                        aCGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCGGGGAGGCGGATTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTcggc                        <  3:76358/149‑1 (MQ=255)                                                                                                                         cGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTcggcg                       <  8:344086/149‑1 (MQ=255)                                                                                                                         cGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTcggcg                       <  8:348416/149‑1 (MQ=255)                                                                                                                         cGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACAACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCa                                          >  2:81395/1‑130 (MQ=255)                                                                                                                         cGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACAACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCa                                          <  1:81395/130‑1 (MQ=255)                                                                                                                          gCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTcggcgg                      >  2:96298/1‑149 (MQ=255)                                                                                                                            tGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGt                    >  7:159263/1‑149 (MQ=255)                                                                                                                                   cgAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGtt                                                          >  3:19929/1‑104 (MQ=255)                                                                                                                                   cgAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGtt                                                          <  4:19929/104‑1 (MQ=255)                                                                                                                                       cTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCgg         <  4:269738/149‑1 (MQ=255)                                                                                                                                               cccTGGCTGGCCTGGGCATGGAAGGTTGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCGGGt  >  6:177114/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                  GTCGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCGGGT  >  NC_002947/3436051‑3436340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑