Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,463,544 G→C 5.6% L272V (CTG→GTG)  PP_3078 ← ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,463,5440GC 5.6% 233.5 / 3.1 71L272V (CTG→GTG) PP_3078ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (35/32);  new base C (2/2);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.21e-01

CGTTGTAGCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCAT  >  NC_002947/3463397‑3463691
                                                                                                                                                   |                                                                                                                                                   
cGTTGTAGCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAgg                                                                                                                                                    >  8:253237/1‑149 (MQ=255)
  ttGTAGCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTc                                                                                                                                                  >  7:179987/1‑149 (MQ=255)
     tAGCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCAc                                                                                                                                                <  4:132020/148‑1 (MQ=255)
     tAGCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACg                                                                                                                                               >  8:83771/1‑149 (MQ=255)
     tAGCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGAACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCAc                                                                                                                                                <  1:98321/148‑1 (MQ=255)
       gCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTg                                                                                                                                             >  7:90398/1‑149 (MQ=255)
        cccAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGc                                                                                                                                            >  7:58722/1‑149 (MQ=255)
         ctaaGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGcc                                                                                                                                           >  4:185902/3‑149 (MQ=255)
         ccAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGcc                                                                                                                                           >  8:239387/1‑149 (MQ=255)
          cAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGTGGGCACGTGACCTGccc                                                                                                                                          >  2:139798/1‑149 (MQ=255)
          cAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGcc                                                                                                                                           >  2:43003/1‑148 (MQ=255)
            aGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCccac                                                                                                                                        >  8:84588/1‑149 (MQ=255)
              gtgtAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGTGGGCACGTGACCTGCccgcca                                                                                                                                      <  1:139798/149‑1 (MQ=255)
                   ggCCGTTGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCAc                                                                                                                                                <  4:353853/134‑1 (MQ=255)
                   ggCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCAc                                                                                                                                                >  3:353853/1‑134 (MQ=255)
                   ggCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGcc                                                                                                                                  <  3:228288/148‑1 (MQ=255)
                                 aCTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGaa                                                                                                                    <  8:90398/148‑1 (MQ=255)
                                 aCTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAg                                                                                                                   <  8:58722/149‑1 (MQ=255)
                                  cTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGaa                                                                                                                    <  2:181349/147‑1 (MQ=255)
                                  cTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGaa                                                                                                                    >  1:181349/1‑147 (MQ=255)
                                   tCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGt                                                                                                                 >  2:169137/1‑149 (MQ=255)
                                   tCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGt                                                                                                                 >  4:57863/1‑149 (MQ=255)
                                     gcgGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACTACGCCGGGTCGCGTTCGAAGGTGa                                                                                                               <  7:239387/149‑1 (MQ=255)
                                         ttGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGAtgct                                                                                                           <  1:305326/149‑1 (MQ=255)
                                              gTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGc                                                                                                      <  4:125002/149‑1 (MQ=255)
                                               tCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGcc                                                                                                     >  3:22947/1‑149 (MQ=255)
                                                 gTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGt                                                                                                   >  1:68306/1‑149 (MQ=255)
                                                 gTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGt                                                                                                   >  8:90909/1‑149 (MQ=255)
                                                  tAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGtt                                                                                                  <  1:169137/149‑1 (MQ=255)
                                                    gCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGt                                                                                                <  7:253237/149‑1 (MQ=255)
                                                     ccACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTc                                                                                               >  7:290677/1‑149 (MQ=255)
                                                     ccACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTc                                                                                               >  5:388592/1‑149 (MQ=255)
                                                      cACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGTGGGCACGTGACCTGcc                                                                                                                                           <  5:24724/104‑1 (MQ=255)
                                                      cACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGTGGGCACGTGACCTGcc                                                                                                                                           >  6:24724/1‑104 (MQ=255)
                                                      cACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCAc                                                                                                                                                >  5:221245/1‑99 (MQ=255)
                                                      cACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCAc                                                                                                                                                <  6:221245/99‑1 (MQ=255)
                                                        ccGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGAt                                                                                            <  3:116118/149‑1 (MQ=255)
                                                          gCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGa                                                                                             >  7:332401/1‑146 (MQ=255)
                                                          gCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGa                                                                                             <  8:332401/146‑1 (MQ=255)
                                                               tGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGcc                                                                                       <  7:90909/147‑1 (MQ=255)
                                                                gAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCaa                                                                                    >  4:138416/1‑149 (MQ=255)
                                                                      cTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGt                                                                              <  3:57863/149‑1 (MQ=255)
                                                                      cTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTAACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCgg                                                                               >  2:99800/1‑148 (MQ=255)
                                                                         gcgcgcCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATg                                                                           <  1:99800/149‑1 (MQ=255)
                                                                         gcgcgcCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATg                                                                           <  7:83771/149‑1 (MQ=255)
                                                                           gcgcCAGTTCGGTGTCGCCGAAGTACTCGATGCGCTGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCg                                                                                <  6:50564/142‑1 (MQ=255)
                                                                           gcgcCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCggagatcc                                                                         >  5:50564/1‑143 (MQ=255)
                                                                                  ttCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGc                                                                  >  8:274055/1‑149 (MQ=255)
                                                                                           gCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCTATGCGGTATGGCCCGCTGCCGACTGGc                                                          <  3:185902/148‑1 (MQ=255)
                                                                                           gCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGc                                                          <  7:84588/148‑1 (MQ=255)
                                                                                           gCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCt                                                         >  4:239969/1‑149 (MQ=255)
                                                                                            ccGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGcc                                                                                                     <  8:326755/104‑1 (MQ=255)
                                                                                            ccGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGcc                                                                                                     >  7:326755/1‑104 (MQ=255)
                                                                                            ccGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCtt                                                        <  4:22947/149‑1 (MQ=255)
                                                                                             cGAAGTTCTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTg                                                       >  7:283937/1‑149 (MQ=255)
                                                                                               aaGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTc                                                     <  1:43003/149‑1 (MQ=255)
                                                                                                    cTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGc                                                >  3:129284/1‑149 (MQ=255)
                                                                                                           cgcAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCAc                                          <  8:290677/148‑1 (MQ=255)
                                                                                                            gcAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCgg                                             >  6:356730/1‑144 (MQ=255)
                                                                                                            gcAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCgg                                             <  5:356730/144‑1 (MQ=255)
                                                                                                            gcAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCAGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCAACg                                        >  7:328096/1‑149 (MQ=255)
                                                                                                                      gAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAg                               <  4:129284/148‑1 (MQ=255)
                                                                                                                      gAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGt                              >  4:205354/1‑149 (MQ=255)
                                                                                                                              aGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCACCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCgctgc                      >  3:52642/1‑149 (MQ=255)
                                                                                                                                cGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGctgctg                    <  3:239969/149‑1 (MQ=255)
                                                                                                                                 ggCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCtgctgc                   <  2:68306/149‑1 (MQ=255)
                                                                                                                                          tGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGc          >  2:100203/1‑149 (MQ=255)
                                                                                                                                            gCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGCTc        >  3:225697/1‑149 (MQ=255)
                                                                                                                                              gggCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTGGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGCTCgg      <  8:179987/149‑1 (MQ=255)
                                                                                                                                                gCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGCTCggg     >  7:100623/1‑148 (MQ=255)
                                                                                                                                                  aGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCAt  <  6:388592/149‑1 (MQ=255)
                                                                                                                                                  aGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTACAGGTCGTGCTCGGGCAt  >  7:349869/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGTTGTAGCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCAT  >  NC_002947/3463397‑3463691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑