Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,475,491 G→T 19.5% H408N (CAC→AAC) ‡ PP_3087 ← excinuclease ABC subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,475,4910GT19.5% 79.3 / 13.6 36H408N (CAC→AAC) ‡PP_3087excinuclease ABC subunit A
Reads supporting (aligned to +/- strand):  ref base G (25/4);  new base T (4/3);  total (29/7)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

TCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCA‑CCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAGCCGAGGCCGAGGTC  >  NC_002947/3475352‑3475635
                                                                                                                                            |                                                                                                                                                 
tCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCA‑CCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAgccggc                                                                                                                                          >  3:375912/1‑149 (MQ=255)
  gCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCA‑CCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAgccggcc                                                                                                                                         <  8:162800/148‑1 (MQ=255)
   ccGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCA‑CCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGa                                                                                                                                       <  5:62313/149‑1 (MQ=255)
   ccGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCA‑CCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGa                                                                                                                                       <  4:142/149‑1 (MQ=255)
                 tACTTCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCA‑CCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCCCTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTa                                                                                                                         >  7:330339/1‑149 (MQ=255)
                 tACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCA‑CCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGAcagtgaa                                                                                                                                >  8:207139/1‑136 (MQ=255)
                 tACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCA‑CCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGAcagtgaa                                                                                                                                <  7:116358/142‑7 (MQ=255)
                 tACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCA‑CCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGAcagtgaa                                                                                                                                >  8:116358/1‑136 (MQ=255)
                 tACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCA‑CCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGAcagtgaa                                                                                                                                <  7:207139/142‑7 (MQ=255)
                      ccaccaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCA‑CCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTa                                                                                                                     >  7:205088/1‑148 (MQ=255)
                        accaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCAGCCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTaaa                                                                                                                   >  1:263953/1‑149 (MQ=255)
                        accaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCA‑CCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAac                                                                                                                  >  3:58634/1‑149 (MQ=255)
                                              tGGTGTCCAGGTCATGCTCCA‑CCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAAtt                                                                                            >  3:161369/1‑148 (MQ=255)
                                                          cATGCTCCA‑CCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAAt                                                                                             >  7:314838/1‑135 (MQ=255)
                                                            tGCTCCA‑CCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCcag                                                                             >  5:210679/1‑149 (MQ=255)
                                                               tCCA‑CCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCcaggcg                                                                          >  5:371236/1‑149 (MQ=255)
                                                                 ca‑ccacGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAg                                                                                                                           >  7:305395/1‑99 (MQ=255)
                                                                  caacacGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCcaggcg                                                                          >  4:101771/4‑147 (MQ=255)
                                                                    ccacGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCcaggcgcagg                                                                      >  7:69576/1‑149 (MQ=255)
                                                                       cGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGt                                                                   >  3:262524/1‑149 (MQ=255)
                                                                           ccccGTGTGGCCAGCTTTTTTGAGTGGCTGCTGGGCTGCCAACAGGGCTTCAGTGTCGGCGGGGTGCACGCGGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGc                                                                <  6:371236/146‑1 (MQ=255)
                                                                            acCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGtt                                                                  >  5:191556/1‑145 (MQ=255)
                                                                                 gTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCAc                                                         >  3:102806/1‑149 (MQ=255)
                                                                                                  ggCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCggg                                        >  6:251812/1‑149 (MQ=255)
                                                                                                         cAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGtgctgc                                 >  7:116707/1‑149 (MQ=255)
                                                                                                           gggCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTgcgc                               >  5:335577/1‑149 (MQ=255)
                                                                                                            ggCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCt                              >  1:189808/1‑149 (MQ=255)
                                                                                                               ttCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTccc                           >  4:301175/1‑148 (MQ=255)
                                                                                                                     cAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGCCGGCGTGCTGCGCTCCAGGGc                       >  3:232427/1‑147 (MQ=255)
                                                                                                                           ttCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCGCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAg                >  1:103988/1‑149 (MQ=255)
                                                                                                                           ttCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGc                                                                                                 >  3:260198/1‑67 (MQ=255)
                                                                                                                               ccgTCGGCCGGCTTGCACCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAgccgag           >  1:275447/3‑149 (MQ=255)
                                                                                                                                 gTCGGCCGGCTTGCACCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTc                                           >  8:332453/1‑115 (MQ=255)
                                                                                                                                     gCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTa                 >  2:46473/1‑137 (MQ=255)
                                                                                                                                     gCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTa                 <  1:46473/137‑1 (MQ=255)
                                                                                                                                          gTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAGCCGAGGCCGAGGTc  >  4:307753/1‑147 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
TCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCA‑CCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACC‑CCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAGCCGAGGCCGAGGTC  >  NC_002947/3475352‑3475635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑