Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 285,328 T→A 6.8% Q41L (CAG→CTG)  atsK ← alpha‑ketoglutarate‑dependent sulfonate dioxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947285,3280TA 6.8% 178.3 / 3.1 59Q41L (CAG→CTG) atsKalpha‑ketoglutarate‑dependent sulfonate dioxygenase
Reads supporting (aligned to +/- strand):  ref base T (28/27);  new base A (2/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

ACATCGGTAACCGCCGTGTCGAGCACAAGCACCTGTGGGGTGTCTGGCACGTTGGGGTAAATCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGAGTGATGGTCAGGCTCATGTCGGTCTCTTCAATCGGGCGCCAGTG  >  NC_002947/285181‑285476
                                                                                                                                                   |                                                                                                                                                    
acaTCGGTAACCGCCGTGTCGAGCACAAGCACCTGTGGGGTGTCTGGCACGTTGGGGTAAATCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTg                                                                                                                                                     <  5:332607/149‑1 (MQ=255)
 caTCGGTAACCGCCGTGTCGAGCACAAGCACCTGTGGGGTGTCTGGCACGTTGGGGTAAATCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTgg                                                                                                                                                    <  3:268916/149‑1 (MQ=255)
     ggTAACCGCCGTGTCGAGCACAAGCACCTGTGGGGTGTCTGGCACGTTGGGGTAAATCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGc                                                                                                                                                 <  1:199019/148‑1 (MQ=255)
      gTAACCGCCGTGTCGAGCACAAGCACCTGTGGGGTGTCTGGCACGTTGGGGTAAATCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTg                                                                                                                                               <  8:179659/149‑1 (MQ=255)
               gtgtCGAGCACAAGCACCTGTGGGGTGTCTGGCACGTTGGGGTAAATCGGGTGGATCTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGc                                                                                                                                      <  8:178085/149‑1 (MQ=255)
                  tCGAGCACAAGCACCTGTGGGGTGTCTGGCACGTTGGGGTAAATCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTg                                                                                                                                   >  2:273257/1‑149 (MQ=255)
                    gAGCACAAGCACCTGTGGGGTGTCTGGCACGTTGGGGTAAATCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGtt                                                                                                                                 >  2:190498/1‑149 (MQ=255)
                      gCACAAGCACCTGTGGGGTGTCTGGCACGTTGGGGTAAATCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTc                                                                                                                                >  7:279223/1‑148 (MQ=255)
                       cacaAGCACGTG‑GGGGGGTCGGGCCCGGTTGGGTAACTCGGGGGGCTGGGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGAt                                                                                                                             <  7:274908/149‑1 (MQ=255)
                               ccTGTGGGGTGTCTGGCACGTTGGGGTAAATCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATcg                                                                                                                      >  2:109320/1‑149 (MQ=255)
                                cTGTGGGGTGTCTGGCACGTTGGGGTAAATCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATcgc                                                                                                                     >  4:167906/1‑149 (MQ=255)
                                            tGGCACGTTGGGGTAAATCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTgcgc                                                                                                         <  8:279223/149‑1 (MQ=255)
                                            tGGCACGTTGGGGTAAATCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTgcgc                                                                                                         >  2:59843/1‑149 (MQ=255)
                                              gCACGTTGGGGTAAATCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCt                                                                                                        <  1:109320/148‑1 (MQ=255)
                                              gCACGTTGGGGTAAATCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCt                                                                                                        <  4:7522/148‑1 (MQ=255)
                                                 cGTTGGGGTAAATCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGTGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGata                                                                                                    >  5:73119/1‑149 (MQ=255)
                                                        gTAAATCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGc                                                                                             >  2:83568/1‑149 (MQ=255)
                                                        gTAAATCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGc                                                                                             >  4:367732/1‑149 (MQ=255)
                                                           aaTCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTg                                                                                           <  1:273257/148‑1 (MQ=255)
                                                               gggTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTc                                                                                      >  5:201480/1‑149 (MQ=255)
                                                                    gATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCAcgc                                                                                 <  3:167906/149‑1 (MQ=255)
                                                                    gATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCAcgc                                                                                 >  5:281233/1‑149 (MQ=255)
                                                                        tgCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCa                                                                                    >  4:160031/1‑142 (MQ=255)
                                                                        tgCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCa                                                                                    <  3:160031/142‑1 (MQ=255)
                                                                             gTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCt                                                                        <  1:59843/149‑1 (MQ=255)
                                                                              tCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTg                                                                       >  4:227263/1‑149 (MQ=255)
                                                                              tCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTg                                                                       <  7:250107/149‑1 (MQ=255)
                                                                                  cGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGgcg                                                                   >  7:109466/1‑149 (MQ=255)
                                                                                         tGCGGCAAAGCGCGACTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTg                                                            >  7:142557/1‑149 (MQ=255)
                                                                                           cGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCt                                                          >  1:190606/1‑149 (MQ=255)
                                                                                                   cgcgcCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAg                                                  <  3:227263/149‑1 (MQ=255)
                                                                                                      gcCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAggg                                                <  6:279956/148‑1 (MQ=255)
                                                                                                      gcCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAggg                                                >  5:279956/1‑148 (MQ=255)
                                                                                                       ccTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGAt                                                                                                                             >  4:41079/1‑70 (MQ=255)
                                                                                                       ccTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGAt                                                                                                                             <  3:41079/70‑1 (MQ=255)
                                                                                                       ccTGCGGGTCCGGGCGGATCGGCAGGGCGCGGACGAACAGCACCTGGTGCGGGAGCAGGGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGACGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGa                                              <  8:93179/149‑1 (MQ=255)
                                                                                                             gTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGAGTGATg                                        >  1:148656/1‑149 (MQ=255)
                                                                                                              ttCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGAGTGAt                                         <  6:201480/147‑1 (MQ=255)
                                                                                                              ttCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGAGTGAt                                         <  8:109466/147‑1 (MQ=255)
                                                                                                              ttCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGAGTGATgg                                       >  3:87602/1‑149 (MQ=255)
                                                                                                                   ggTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTc                                                                                      <  6:73190/97‑1 (MQ=255)
                                                                                                                   ggTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTc                                                                                      >  5:73190/1‑97 (MQ=255)
                                                                                                                    gTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGAGTGATGGTTAGGc                                 >  3:216436/1‑149 (MQ=255)
                                                                                                                        aTCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTgg                                                        >  5:388686/1‑122 (MQ=255)
                                                                                                                        aTCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTgg                                                        <  6:388686/122‑1 (MQ=255)
                                                                                                                        aTCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGAGTGATGGTCAGGCTCAt                             >  7:317411/1‑149 (MQ=255)
                                                                                                                           ggCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGAGTGATGGTCAGGCTCATGt                           <  2:190606/148‑1 (MQ=255)
                                                                                                                               ggTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGAGTGATGGTCAGGCTCATGTCGGtc                      >  6:157599/1‑149 (MQ=255)
                                                                                                                                   gcgGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGAGTGATGGTCAGGCTCATGTCGGTctct                   <  6:251056/148‑1 (MQ=255)
                                                                                                                                   gcgGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGAGTGATGGTCAGGCTCATGTCGGTctct                   <  6:245544/148‑1 (MQ=255)
                                                                                                                                         gaACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTggg                                                       >  5:327446/1‑106 (MQ=255)
                                                                                                                                         gaACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTggg                                                       <  6:327446/106‑1 (MQ=255)
                                                                                                                                          tccAGCACCAGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTg                                                                                                       <  7:61912/55‑1 (MQ=255)
                                                                                                                                          tccAGCACCAGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTg                                                                                                       >  8:61912/3‑57 (MQ=255)
                                                                                                                                          tccAGCACCAGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGAGTGATGGTCAGGCTCATGTCGGTCTCTTCAATCgg           >  8:88154/3‑149 (MQ=255)
                                                                                                                                           aCAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGAGTGATGGTCAGGCTCATGTCGGTCTCTTCAATCggg          >  7:205180/1‑149 (MQ=255)
                                                                                                                                           aCAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGAGTGATGGTCAGGCTCATGTCGGTCTCTTCAATCggg          >  6:4989/1‑149 (MQ=255)
                                                                                                                                            cAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGAGTGATGGTCAGGCTCATGTCGGTCTCTTCAATCGGgc         <  3:367732/149‑1 (MQ=255)
                                                                                                                                            cAGCACCAGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGAGTGATGGTCAGGCTCATGTCGGTCTCTTCAATCgg           <  7:88154/147‑1 (MQ=255)
                                                                                                                                                   tGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGAGTGATGGTCAGGCTCATGTCGGTCTCTTCAATCGGGCGCCAgtg  >  8:210219/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACATCGGTAACCGCCGTGTCGAGCACAAGCACCTGTGGGGTGTCTGGCACGTTGGGGTAAATCGGGTGGATGTGCAGGTCGCCGAAGCGTGCGGCAAAGCGCGCCTGCTGTTCCGGGTTGATCGGCTGGTCGCGGAGGAACAGCACCTGGTGCTGGAGCAGTGCCTGTTCGATGGCATCGCGCTCTTCTGCGCTGATATCGCGGCTGATGTCCACGCCGCTGATCTGGGCGCCGAGTGCTGGGCTTAGGGGAGTGATGGTCAGGCTCATGTCGGTCTCTTCAATCGGGCGCCAGTG  >  NC_002947/285181‑285476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑