Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,525,860 G→A 8.2% L102L (TTG→TTA) ‡ lexA‑II → transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,525,8600GA 8.2% 175.5 / 4.5 61L102L (TTG→TTA) ‡lexA‑IItranscriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (31/25);  new base A (2/3);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01

TTACATCGATGTCACGCCAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCCGCGACGGGCAAATCGTGGTTG  >  NC_002947/3525716‑3526005
                                                                                                                                                |                                                                                                                                                 
ttACATCGATGTCACGCCAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTtgctgc                                                                                                                                               <  7:303168/149‑1 (MQ=255)
       gATGTCACGCCAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGCAACTGCTCgtgg                                                                                                                                         <  8:179977/148‑4 (MQ=255)
            cACGCAAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGca                                                                                                                                   >  7:245332/1‑148 (MQ=255)
               gCCAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGt                                                                                                                                >  1:339893/1‑149 (MQ=255)
                ccAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGtt                                                                                                                               <  7:76851/149‑1 (MQ=255)
                ccAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGGTGCGGCGGGTGCCCCCATCGTCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGtt                                                                                                                               <  7:71700/149‑1 (MQ=255)
                 cAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTc                                                                                                                              >  5:17203/1‑149 (MQ=255)
                  aaaCCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTcc                                                                                                                             <  2:339893/149‑1 (MQ=255)
                           gcgGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGCCCCCAGCCTGTTCCGCCGTAccc                                                                                                                    >  7:89568/1‑149 (MQ=255)
                               ggCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGa                                                                                                                >  4:74907/1‑149 (MQ=255)
                                gtaTACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTccgcc                                                                                                                          >  2:60271/3‑138 (MQ=255)
                                gtaTACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTccgcc                                                                                                                          <  1:60271/136‑1 (MQ=255)
                                  aTACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTa                                                                                                             >  8:263802/1‑149 (MQ=255)
                                   tACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTAc                                                                                                            <  2:242068/149‑1 (MQ=255)
                                   tACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTAc                                                                                                            >  5:120769/1‑149 (MQ=255)
                                       ccTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACctgc                                                                                                        >  1:331016/1‑149 (MQ=255)
                                           gCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACctgctg                                                                                                      >  4:39685/1‑147 (MQ=255)
                                           gCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACctgctg                                                                                                      >  4:10967/1‑147 (MQ=255)
                                                  cGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCgg                                                                                             <  3:10967/149‑1 (MQ=255)
                                                       cgcCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGc                                                                                          <  6:120769/147‑1 (MQ=255)
                                                        gccggctgGAAATCCTCGAAATAGCAGAGCTGGGCCAGGTGGCGGCGGGTGGCCCCATCGGCCCGGTCCTGGGCATGCACGAGCAGTTGCTGGTCGACCCCAGCCTGTTGCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGAc                                                                                       <  8:245332/149‑1 (MQ=255)
                                                         ccggccggAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACt                                                                                      <  4:150947/149‑1 (MQ=255)
                                                             ccggAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCgat                                                                                  <  6:17203/149‑1 (MQ=255)
                                                                    tCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATcgac                                                                           <  3:39685/149‑1 (MQ=255)
                                                                      cTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTAc                                                                                                            >  6:259477/1‑114 (MQ=255)
                                                                      cTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTAc                                                                                                            <  5:259477/114‑1 (MQ=255)
                                                                      cTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATcgacga                                                                         <  7:263802/149‑1 (MQ=255)
                                                                       tCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCgacgac                                                                        >  3:268413/1‑149 (MQ=255)
                                                                       tCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGCCCCGCACCTGGGCATCCACCACCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGCCTCCCTGCTCAAGGTGCGGGGCGACACCAGGATCgacgac                                                                        >  4:293614/1‑149 (MQ=255)
                                                                            aTACCCGTGCGTGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCAt                                                                   <  3:293614/149‑1 (MQ=255)
                                                                                ccGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCttt                                                               >  1:350122/1‑149 (MQ=255)
                                                                                ccGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCttt                                                               >  1:209095/1‑149 (MQ=255)
                                                                                        ggCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGa                                                       <  3:74907/149‑1 (MQ=255)
                                                                                        ggCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGa                                                       >  7:51930/1‑149 (MQ=255)
                                                                                          ccAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGAcc                                                     <  2:209095/149‑1 (MQ=255)
                                                                                                 gcggcgGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCgg                                              >  1:70338/1‑149 (MQ=255)
                                                                                                        gTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTg                                       <  5:79470/149‑1 (MQ=255)
                                                                                                           cccccATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGa                                                             <  1:208104/124‑1 (MQ=255)
                                                                                                           cccccATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGa                                                             >  2:208104/1‑124 (MQ=255)
                                                                                                            ccccATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTAcccc                                                                                                                   >  3:325504/1‑69 (MQ=255)
                                                                                                            ccccATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTAcccc                                                                                                                   <  4:325504/69‑1 (MQ=255)
                                                                                                            ccccATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGCCTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCaa                                    >  5:220203/1‑148 (MQ=255)
                                                                                                            ccccATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAAc                                   <  2:350122/149‑1 (MQ=255)
                                                                                                             cccANCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCaa                                    >  5:137945/1‑147 (MQ=255)
                                                                                                              ccATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAg                                 >  6:296586/1‑149 (MQ=255)
                                                                                                                    gCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGAc                           <  2:331016/149‑1 (MQ=255)
                                                                                                                     cccGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACg                          >  1:116490/1‑149 (MQ=255)
                                                                                                                      ccGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGc                         >  2:134612/1‑149 (MQ=255)
                                                                                                                      ccGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGc                         >  3:16874/1‑149 (MQ=255)
                                                                                                                          aCCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCcgc                     >  7:196214/1‑149 (MQ=255)
                                                                                                                              gggCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATc                                          >  3:337351/1‑124 (MQ=255)
                                                                                                                              gggCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATc                                          <  4:337351/124‑1 (MQ=255)
                                                                                                                                 cATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCCGCGACGGGc              <  8:196214/149‑1 (MQ=255)
                                                                                                                                     gtcGAGCAGCAACTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCg                                               <  7:212731/110‑1 (MQ=255)
                                                                                                                                     gtcGAGCAGCAACTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCg                                               >  8:212731/3‑112 (MQ=255)
                                                                                                                                     gtcGAGCAGCAACTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCg                                               >  6:141591/3‑112 (MQ=255)
                                                                                                                                     gtcGAGCAGCAACTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCg                                               <  5:141591/110‑1 (MQ=255)
                                                                                                                                           cAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGACATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCCGCGACGGGCAAATCGTGGt    >  4:49584/1‑149 (MQ=255)
                                                                                                                                             gTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCCGCGACGGGCAAATCGTGGTTg  >  2:189988/1‑149 (MQ=255)
                                                                                                                                             gTTGCTGCGCGACCCCAGCCTGTGCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCCGCGACGGGCAAATCGTGGTTg  <  6:220203/149‑1 (MQ=255)
                                                                                                                                              ttGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTg                                                   >  7:227279/1‑99 (MQ=255)
                                                                                                                                              ttGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTg                                                   <  8:227279/99‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TTACATCGATGTCACGCCAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCCGCGACGGGCAAATCGTGGTTG  >  NC_002947/3525716‑3526005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑