| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,534,555 | T→C | 7.0% | C167R (TGC→CGC) | atoB → | 3‑oxoacid CoA‑transferase subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,534,555 | 0 | T | C | 7.0% | 166.5 / 2.1 | 57 | C167R (TGC→CGC) | atoB | 3‑oxoacid CoA‑transferase subunit B |
| Reads supporting (aligned to +/- strand): ref base T (24/29); new base C (2/2); total (26/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCCGGGCAAGCTGGTCAAGGGCATGGGCGGGGCCATGGACCTGGTGGCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGA > NC_002947/3534409‑3534700 | ttCCGGGCAAGCTGGTCAAGGGCATGGGCGGGGCCATGGACCTGGTGGCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGTGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTg > 5:139840/1‑148 (MQ=255) gggCAAGCTGGTCAAGGGCATGGGCGGGGCCATGGACCTGGTGGCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATcc < 1:26353/149‑1 (MQ=255) aaGCTGGTCAAGGGCATGGGCGGGGCCATGGACCTGGTGGCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCaa < 6:6324/149‑1 (MQ=255) gCTGGTCAAGGGCATGGGCGGGGCCATGGACCTGGTGGCCGGTGCGGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCCGCCTGCCCCTGACC‑GGTGCGGGCCGCATCCGAAAgg > 6:93030/1‑149 (MQ=255) gCTGGTCAAGGGCATGGGCGGGGCCATGGACCTGGTGGCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCCGCATCCGCAAgg > 6:93034/1‑149 (MQ=255) tCAAGGGCATGGGCGGGGCCATGGACCTGGTGGCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCt > 8:234099/1‑148 (MQ=255) aaGGGCATGGGCGGGGCCATGGACCTGGTGGCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTgac > 6:248924/1‑149 (MQ=255) aaGGGCATGGGCGGGGCCATGGACCTGGTGGCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTga < 5:245917/148‑1 (MQ=255) aaGGGCATGGGCGGGGCCATGGACCTGGTGGCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTga < 7:234099/148‑1 (MQ=255) gggCATGGGCGGGGCCATGGACCTGGTGGCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTgac > 6:175179/1‑147 (MQ=255) gggCATGGGCGGGGCCATGGACCTGGTGGCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTgac < 5:175179/147‑1 (MQ=255) cATGGGCGGGGCCATGGACCTGGTGGCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGTCGAGTCGAAGTTGATGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTgaccgac < 5:271718/148‑1 (MQ=255) tGGGCGGGGCCATGGACCTGGTGGCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTg < 1:181708/149‑1 (MQ=255) gggCCATGGACCTGGTGGCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTAc < 7:125652/149‑1 (MQ=255) cTGGTGGCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCg > 6:114335/1‑148 (MQ=255) gtggCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGaa < 6:314401/147‑1 (MQ=255) gtggCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGaa < 3:210023/147‑1 (MQ=255) gCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACgg < 6:11141/149‑1 (MQ=255) cGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCa < 5:114335/149‑1 (MQ=255) aacaTCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCt > 6:142090/1‑149 (MQ=255) catcatCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGgcgc < 6:261724/138‑1 (MQ=255) catcatCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGgcgc > 5:261724/1‑138 (MQ=255) tcatcGTCACCATGTCCCACGCCTCCAAGGACGGCGAGTCCAAGTTTCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCCGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTgcgc < 5:93030/149‑1 (MQ=255) tcatcGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCCGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTgcgc < 5:93034/149‑1 (MQ=255) catcGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTgcgc > 3:188597/1‑148 (MQ=255) atcGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGa < 8:321245/128‑1 (MQ=255) atcGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGa > 7:321245/1‑128 (MQ=255) atcGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCga < 3:77855/149‑1 (MQ=255) tCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGAcc > 3:201316/1‑149 (MQ=255) cACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCgaga < 8:244079/146‑1 (MQ=255) cccACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTggg < 5:248924/149‑1 (MQ=255) aCGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTgg < 5:115010/93‑1 (MQ=255) aCGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTgg > 6:115010/1‑93 (MQ=255) aaGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTggg > 4:42314/1‑138 (MQ=255) aaGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTggg < 1:262018/138‑1 (MQ=255) aaGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTggg > 2:262018/1‑138 (MQ=255) aaGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTggg < 3:42314/138‑1 (MQ=255) aaGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTc > 3:363619/1‑147 (MQ=255) cagCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGaaa < 7:134967/147‑1 (MQ=255) cGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGaaa < 5:142090/149‑1 (MQ=255) tgcCAAAGTGCAGCCTGCCGCTGACCGGGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAAccgc > 1:209908/1‑149 (MQ=255) ccAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCt > 7:128158/1‑88 (MQ=255) ccAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCt < 8:128158/88‑1 (MQ=255) cAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATAGAGAAAACCGCCgg > 7:157086/1‑148 (MQ=255) cAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGa > 6:62714/1‑149 (MQ=255) tGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTg > 3:153525/1‑149 (MQ=255) ccGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGcc < 6:161751/149‑1 (MQ=255) ccGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGc < 6:139840/148‑1 (MQ=255) cGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCg > 1:156948/1‑149 (MQ=255) gCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCg > 5:129225/1‑148 (MQ=255) gACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGgat > 6:36513/1‑148 (MQ=255) aCC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCaaa < 7:147740/132‑1 (MQ=255) aCC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCaaa > 8:147740/1‑132 (MQ=255) aCC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGgatg < 4:363619/148‑1 (MQ=255) gggCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTATGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGTAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAg > 2:304698/1‑148 (MQ=255) gggCTGCATCCGCAAGGTGCTGACCGACCGGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAgg < 8:289634/149‑1 (MQ=255) ggCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGa < 4:188597/149‑1 (MQ=255) | TTCCGGGCAAGCTGGTCAAGGGCATGGGCGGGGCCATGGACCTGGTGGCCGGTGCCGACAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGTTGCTGCCAAAGTGCAGCCTGCCGCTGACC‑GGTGCGGGCTGCATCCGCAAGGTGCTGACCGACCTGGCCTACCTGGAAATCGAAGACGGCGCCTTCATTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGA > NC_002947/3534409‑3534700 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |