Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,600,609	A→T	5.4%	L195Q (CTG→CAG)	PP_3176 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,600,609	0	A	T	5.4%	232.4 / 2.5	74	L195Q (CTG→CAG)	PP_3176	MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (36/34);  new base T (2/2);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.40e-01

CTGTCGCTGGTGGTGCTCGGTTTGCTTGCAGCATCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGCCCGATACCGGGATCGCCGACATGAAAAGGGCCATGATGCGGCCGCGCCG  >  NC_002947/3600462‑3600757                                                                                                                                                    |                                                                                                                                                     cTGTCGCTGGTGGTGCTCGGTTTGCTTGCAGCATCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAg                                                                                                                                                     <  5:222763/149‑1 (MQ=255)    tCGCTGGTGGTGCTCGGTTTGCTTGCAGCATCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAgg                                                                                                                                                    <  7:361042/147‑1 (MQ=255)       cTGGTGGTGCTCGGTTTGCTTGCAGCATCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTg                                                                                                                                               >  8:228552/1‑149 (MQ=255)          gtggtgCTCGGTTTGCTTGCAGCATCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCT‑GTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATc                                                                                                                                           <  1:16963/149‑1 (MQ=255)            ggtgCTCGGTTTGCTTGCAGCATCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGAt                                                                                                                                            >  7:326793/1‑147 (MQ=255)               gCTCGGTTTGCTTGCAGCATCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCa                                                                                                                                          >  1:221268/1‑146 (MQ=255)               gCTCGGTTTGCTTGCAGCATCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCa                                                                                                                                          <  2:221268/146‑1 (MQ=255)                cTCGGTTTGCTTGCAGCATCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGaa                                                                                                                                      <  6:184823/149‑1 (MQ=255)                cTCGGTTTGCTTGCAGCATCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGaa                                                                                                                                      <  8:103887/149‑1 (MQ=255)                cTCGGTTTGCTTGCAGCATCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGa                                                                                                                                       <  1:64656/148‑1 (MQ=255)                    gTTTGCTTGCAGCATCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATc                                                                                                                                  >  3:94994/1‑149 (MQ=255)                    gTTTGCTTGCAGCATCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATc                                                                                                                                  >  1:179055/1‑149 (MQ=255)                        gcttgcAGCATCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCAc                                                                                                                               <  2:179055/148‑1 (MQ=255)                         cttgcAGCATCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTg                                                                                                                             >  5:58810/1‑149 (MQ=255)                          ttgcAGCATCGGCCTGCAGGTCGGCAGCCATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGc                                                                                                                            >  1:190981/1‑149 (MQ=255)                            gcagcaTCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCa                                                                                                                          >  4:9453/1‑149 (MQ=255)                             cagcaTCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGa                                                                                                                                             <  8:82656/129‑1 (MQ=255)                             cagcaTCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGa                                                                                                                                             >  7:82656/1‑129 (MQ=255)                              agcaTCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCTGGCCCTGGATCAGGAAc                                                                                                                                     <  5:355568/136‑1 (MQ=255)                              agcaTCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCTGGCCCTGGATCAGGAAc                                                                                                                                     >  6:355568/1‑136 (MQ=255)                               gcaTCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGcc                                                                                                                       <  8:342211/149‑1 (MQ=255)                                caTCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCg                                                                                                                      <  5:373620/149‑1 (MQ=255)                                      ccTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAgg                                                                                                                >  2:5374/1‑149 (MQ=255)                                       cTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGc                                                                                                               <  3:272470/149‑1 (MQ=255)                                          cAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGt                                                                                                            <  1:95694/149‑1 (MQ=255)                                          cAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCACAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGt                                                                                                            >  4:274474/1‑149 (MQ=255)                                           aGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCTGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTg                                                                                                           >  4:39933/1‑149 (MQ=255)                                              tCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTg                                                                                                        <  1:5374/149‑1 (MQ=255)                                                ggCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGc                                                                                                      <  8:326793/149‑1 (MQ=255)                                                  cAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCcg                                                                                                    >  2:2513/1‑149 (MQ=255)                                                    gCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCcggc                                                                                                  <  8:21358/149‑1 (MQ=255)                                                     cGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCTGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCcggcg                                                                                                 <  3:39933/149‑1 (MQ=255)                                                     cGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCcggcg                                                                                                 <  7:228552/149‑1 (MQ=255)                                                     cGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCcggcg                                                                                                 <  4:94994/149‑1 (MQ=255)                                                      gATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCcggcgg                                                                                                <  5:109713/149‑1 (MQ=255)                                                       aTCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAgg                                                                                                                                        >  6:143710/1‑108 (MQ=255)                                                       aTCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAgg                                                                                                                                        <  5:143710/108‑1 (MQ=255)                                                            cTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAAt                                                                                          >  6:29722/1‑149 (MQ=255)                                                            cTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAAt                                                                                          >  4:301427/1‑149 (MQ=255)                                                                 gTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGc                                                                                                                    >  8:259738/1‑118 (MQ=255)                                                                 gTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGc                                                                                                                    <  7:259738/118‑1 (MQ=255)                                                                 gTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTc                                                                                      >  8:275532/1‑148 (MQ=255)                                                                  ttCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGTCGTGCTGGCCGGCGGCGAAATGTTCGa                                                                                    <  6:17768/149‑1 (MQ=255)                                                                  ttCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTc                                                                                      >  5:272226/1‑147 (MQ=255)                                                                  ttCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTc                                                                                      >  3:279096/1‑147 (MQ=255)                                                                             tCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGACCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCg                                                                         >  4:176731/1‑149 (MQ=255)                                                                                          tttCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGcc                                                                          <  5:125213/135‑1 (MQ=255)                                                                                          tttCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGcc                                                                          >  6:125213/1‑135 (MQ=255)                                                                                          tttCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGc                                                            >  7:235282/1‑149 (MQ=255)                                                                                             cTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGcc                                                           >  7:5104/1‑147 (MQ=255)                                                                                              tGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAg                                                        >  1:178399/1‑149 (MQ=255)                                                                                                gTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGc                                                       <  4:135005/148‑1 (MQ=255)                                                                                                gTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCa                                                      <  3:9453/149‑1 (MQ=255)                                                                                                     cGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCggcggc                                                                                               <  2:201957/103‑1 (MQ=255)                                                                                                     cGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCggcggc                                                                                               >  1:201957/1‑103 (MQ=255)                                                                                                     cGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGccc                                                 <  3:274474/149‑1 (MQ=255)                                                                                                     cGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGccc                                                 <  1:2513/149‑1 (MQ=255)                                                                                                     cGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGccc                                                 <  8:85478/149‑1 (MQ=255)                                                                                                         aCTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGCCCGATa                                             >  7:100490/1‑149 (MQ=255)                                                                                                             aGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGACCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGCCCGATACCg                                          <  3:176731/148‑1 (MQ=255)                                                                                                                aGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGCCCGATACCGGGAt                                      >  4:307073/1‑149 (MQ=255)                                                                                                                    acacCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGCCCGATACCGGGATCGcc                                  >  2:30074/1‑149 (MQ=255)                                                                                                                     cacCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGCCCGATACCGGGATCGCCg                                 <  8:235282/149‑1 (MQ=255)                                                                                                                       ccGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGCCCGATACCGGGATCGCCGAc                               <  4:279096/149‑1 (MQ=255)                                                                                                                        cGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGCCCGATACCGGGATCGCCGACa                              >  6:310897/1‑149 (MQ=255)                                                                                                                           cAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGCCCGATACCGGGATCGCCGACATg                            >  7:29051/1‑148 (MQ=255)                                                                                                                              tACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGCCCGATACCGGGATCGCCGACATGaaaa                        <  2:190981/149‑1 (MQ=255)                                                                                                                              tACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGCCCGATACCGGGATCGCCGACATGaa                          >  8:235272/1‑147 (MQ=255)                                                                                                                                cGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGCCCGATACCGGGATCGCCGACATGAAAAgg                      >  3:145545/1‑149 (MQ=255)                                                                                                                                 gCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGCCCGATACCGGGATCGCCGACATGAAAAggg                     >  6:162182/1‑149 (MQ=255)                                                                                                                                  cccAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGCCCGATACCGGGATCGCCGACATGAAAAGGGc                    >  3:145698/1‑149 (MQ=255)                                                                                                                                          cACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGCCCGATACCGGGATCGCCGACATGAAAAGGGCCATGATGc            >  6:337412/1‑149 (MQ=255)                                                                                                                                          cACGGTCGGCAGGCCCTGGACCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGCCCGATACCGGGATCGCCGACATGAAAAGGGCCATGATGc            >  8:325911/1‑149 (MQ=255)                                                                                                                                              gTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGCCCGATACCGGGATCGCCGACATGAAAAGGGCCATGATGCGGcc        <  1:30074/149‑1 (MQ=255)                                                                                                                                                    aGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACCGCCCGCCAAGCAGGCCCGATACCGGGATCGCCGACATGAAAAGGGCCATGATGCGGCCGCGCCg  >  5:38310/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CTGTCGCTGGTGGTGCTCGGTTTGCTTGCAGCATCGGCCTGCAGGTCGGCAGCGATCAGCTGACGTTCAGCCGGGCTCAGCCAGGCGGCTTTCTGGTAGCCGTCACTGAGCAGGAACACCGCCAGTACGCCCAAGGCCACGGTCGGCAGGCCCTGGATCAGGAACATCCACTGCCAGCCGGCCAGGCCGTGCTGGCCGGCGGCGAAATGTTCGAGGATCCAGCCGGAGAACGGCCCGCCAAGCAGGCCCGATACCGGGATCGCCGACATGAAAAGGGCCATGATGCGGCCGCGCCG  >  NC_002947/3600462‑3600757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑