Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,627,068 A→C 9.3% D97A (GAC→GCC)  PP_3197 → glyoxalase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,627,0680AC 9.3% 154.1 / 5.5 52D97A (GAC→GCC) PP_3197glyoxalase family protein
Reads supporting (aligned to +/- strand):  ref base A (23/24);  new base C (2/3);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCATTATCCGCCGCGGCGACGACCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGATCAACCAGCTGGGCATT  >  NC_002947/3626920‑3627213
                                                                                                                                                    |                                                                                                                                                  
aTCATTATCCGCCGCGGCGACGACCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGa                                                                                                                                                    <  5:183748/149‑1 (MQ=255)
   aTTATCCGCCGCGGCGACGACCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGc                                                                                                                                                 <  5:219352/149‑1 (MQ=255)
    ttATCCGCCGCGGCGACGACCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGcc                                                                                                                                                >  3:205428/1‑149 (MQ=255)
          gccgcGGCGACGACCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAgcggc                                                                                                                                          >  5:174959/1‑149 (MQ=255)
           ccgcGGCGACGACCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAgcggcg                                                                                                                                         <  4:112610/149‑1 (MQ=255)
                gcgACGACCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTg                                                                                                                                    <  2:102905/149‑1 (MQ=255)
                    cgacCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCAACGCGCCGCTGGCGTCGCGCCGcact                                                                                                                                <  3:304760/149‑2 (MQ=255)
                     gacCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCacac                                                                                                                               >  6:256930/1‑149 (MQ=255)
                     gacCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGAACGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCacac                                                                                                                               >  1:278968/1‑149 (MQ=255)
                      acCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCAACGCGCCGCTGGCGTCGCGCCGcacttc                                                                                                                              <  1:251638/149‑4 (MQ=255)
                       cccTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCAACGCGCCGCTGGCGTCGCGCCGcacttcg                                                                                                                             <  8:66032/149‑5 (MQ=255)
                            gCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTcga                                                                                                                         <  4:205428/148‑1 (MQ=255)
                            gCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTcg                                                                                                                          <  7:34724/147‑1 (MQ=255)
                                 ccgccAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCt                                                                                                                   >  4:341535/1‑149 (MQ=255)
                                 ccgccAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTAAGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATAGCGAAGTGCGGCGCGCCGCCAGCGTCGCGTTGAACACCTTCgacgac                                                                                                                     >  2:153557/1‑147 (MQ=255)
                                   gccAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTgg                                                                                                                 <  3:291349/149‑1 (MQ=255)
                                    ccAGCGCGGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTggg                                                                                                                <  7:94642/149‑1 (MQ=255)
                                       gcgcTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCtt                                                                                                             >  4:365193/1‑149 (MQ=255)
                                            ggggCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCa                                                                                                        <  6:174959/149‑1 (MQ=255)
                                             gggCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCa                                                                                                        <  3:341535/148‑1 (MQ=255)
                                                     cccATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGc                                                                                                                                                 >  2:291260/1‑99 (MQ=255)
                                                       cATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTTATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAg                                                                                            <  7:111693/149‑1 (MQ=255)
                                                        aTCGCTGCGCGAGACCGTTTACGGCGTCTCTTCGAGCACCCATCTGGCGGCCATCGCCGACGAGCTGGGCCGAGATCGCGAAGTGCGGCGCGACGACAGCGGCGCGTTGCACACCTTCGACGACCTGGGCGTTGCCAT‑TGCCTTCCAgg                                                                                           >  5:76163/1‑149 (MQ=255)
                                                          cGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCCCCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTg                                                                                         <  6:215585/149‑1 (MQ=255)
                                                           gCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGa                                                                                        <  7:224237/149‑1 (MQ=255)
                                                               cgcgAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTg                                                                                    >  7:221531/1‑149 (MQ=255)
                                                                gcgAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGc                                                                                   >  1:80255/1‑149 (MQ=255)
                                                                   agaCCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGt                                                                                >  2:202539/1‑149 (MQ=255)
                                                                   agaCCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGt                                                                                >  2:208584/1‑149 (MQ=255)
                                                                    gaCCGTTTACGGCGTGGCTTCGAGCACGGATGTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTc                                                                               <  6:76163/149‑1 (MQ=255)
                                                                        gTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGc                                                                                                                                   <  1:359082/94‑1 (MQ=255)
                                                                        gTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGc                                                                                                                                   >  2:359082/1‑94 (MQ=255)
                                                                          ttACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCt                                                                         >  2:113030/1‑149 (MQ=255)
                                                                            aCGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCtt                                                                        <  1:200160/148‑1 (MQ=255)
                                                                            aCGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTc                                                                       >  6:175018/1‑149 (MQ=255)
                                                                             cGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTc                                                                       <  3:23794/148‑1 (MQ=255)
                                                                                 gTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGAcgc                                                                  <  8:221531/149‑1 (MQ=255)
                                                                                      ttCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCgccgg                                                             >  3:239142/1‑149 (MQ=255)
                                                                                            cACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGAc                                                        <  2:15910/148‑1 (MQ=255)
                                                                                            cACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGAc                                                        >  1:15910/1‑148 (MQ=255)
                                                                                                      gcggcCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCaa                                                <  2:80255/146‑1 (MQ=255)
                                                                                                             tCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCATCCGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGTCGACCTTACCAACGCCCCCgg                                       <  1:113030/149‑1 (MQ=255)
                                                                                                                ccGACGAGCT‑GGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCgccggc                                                            <  7:316908/123‑1 (MQ=255)
                                                                                                                ccGACGAGCT‑GGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCgccggc                                                            >  8:316908/1‑123 (MQ=255)
                                                                                                                     gaGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCAca                              >  6:164083/1‑148 (MQ=255)
                                                                                                                             ccGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGcc                      >  7:125899/1‑149 (MQ=255)
                                                                                                                               gTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCg                    <  8:125899/149‑1 (MQ=255)
                                                                                                                                tGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGa                   <  5:175018/149‑1 (MQ=255)
                                                                                                                                   tCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGATc                 >  4:374042/1‑148 (MQ=255)
                                                                                                                                    cgcgAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGATCaa               >  6:329493/1‑149 (MQ=255)
                                                                                                                                     gcgAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGATCAAc              <  3:365193/149‑1 (MQ=255)
                                                                                                                                             caacgcgCCGCTGGCGTCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGATCAACCAGCTggg      >  2:89684/4‑149 (MQ=255)
                                                                                                                                               gcgcGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGATCAACCAGCTGGGCa    >  5:342073/1‑149 (MQ=255)
                                                                                                                                                 gcgcCGCCCGCGGCGCGTTGCACACCGTCGACGACCTGGGCTTTGCCAT‑TGCCGTCCAGGTGACCTGCCGTCGCACCTTCGACGCGCCGGCCGACCATCCCCACGCCCCCGGTAGCGCACCGCCGCCCCCCAGCCACCAGCAGGGCaag  >  4:100627/1‑147 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATCATTATCCGCCGCGGCGACGACCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGATCAACCAGCTGGGCATT  >  NC_002947/3626920‑3627213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑