Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,627,077 G→T 8.9% G100V (GGC→GTC)  PP_3197 → glyoxalase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,627,0770GT 8.9% 160.5 / 2.5 52G100V (GGC→GTC) PP_3197glyoxalase family protein
Reads supporting (aligned to +/- strand):  ref base G (23/24);  new base T (2/3);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGCGGCGACGACCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGATCAACCAGCTGGGCATTGCCCCGGA  >  NC_002947/3626930‑3627221
                                                                                                                                                   |                                                                                                                                                 
gccgcGGCGACGACCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAgcggc                                                                                                                                                  >  5:174959/1‑149 (MQ=255)
 ccgcGGCGACGACCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAgcggcg                                                                                                                                                 <  4:112610/149‑1 (MQ=255)
      gcgACGACCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTg                                                                                                                                            <  2:102905/149‑1 (MQ=255)
          cgacCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCAACGCGCCGCTGGCGTCGCGCCGcact                                                                                                                                        <  3:304760/149‑2 (MQ=255)
           gacCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCacac                                                                                                                                       >  6:256930/1‑149 (MQ=255)
           gacCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGAACGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCacac                                                                                                                                       >  1:278968/1‑149 (MQ=255)
            acCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCAACGCGCCGCTGGCGTCGCGCCGcacttc                                                                                                                                      <  1:251638/149‑4 (MQ=255)
             cccTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCAACGCGCCGCTGGCGTCGCGCCGcacttcg                                                                                                                                     <  8:66032/149‑5 (MQ=255)
                  gCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTcga                                                                                                                                 <  4:205428/148‑1 (MQ=255)
                  gCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTcg                                                                                                                                  <  7:34724/147‑1 (MQ=255)
                       ccgccAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCt                                                                                                                           >  4:341535/1‑149 (MQ=255)
                       ccgccAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTAAGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATAGCGAAGTGCGGCGCGCCGCCAGCGTCGCGTTGAACACCTTCgacgac                                                                                                                             >  2:153557/1‑147 (MQ=255)
                         gccAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTgg                                                                                                                         <  3:291349/149‑1 (MQ=255)
                          ccAGCGCGGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTggg                                                                                                                        <  7:94642/149‑1 (MQ=255)
                             gcgcTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCtt                                                                                                                     >  4:365193/1‑149 (MQ=255)
                                  ggggCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCa                                                                                                                <  6:174959/149‑1 (MQ=255)
                                   gggCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCa                                                                                                                <  3:341535/148‑1 (MQ=255)
                                             cATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTTATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAg                                                                                                    <  7:111693/149‑1 (MQ=255)
                                              aTCGCTGCGCGAGACCGTTTACGGCGTCTCTTCGAGCACCCATCTGGCGGCCATCGCCGACGAGCTGGGCCGAGATCGCGAAGTGCGGCGCGACGACAGCGGCGCGTTGCACACCTTCGACGACCTGGGCGTTGCCAT‑TGCCTTCCAgg                                                                                                   >  5:76163/1‑149 (MQ=255)
                                                cGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCCCCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTg                                                                                                 <  6:215585/149‑1 (MQ=255)
                                                 gCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGa                                                                                                <  7:224237/149‑1 (MQ=255)
                                                     cgcgAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTg                                                                                            >  7:221531/1‑149 (MQ=255)
                                                      gcgAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGc                                                                                           >  1:80255/1‑149 (MQ=255)
                                                         agaCCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGt                                                                                        >  2:208584/1‑149 (MQ=255)
                                                         agaCCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGt                                                                                        >  2:202539/1‑149 (MQ=255)
                                                          gaCCGTTTACGGCGTGGCTTCGAGCACGGATGTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTc                                                                                       <  6:76163/149‑1 (MQ=255)
                                                              gTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGc                                                                                                                                           <  1:359082/94‑1 (MQ=255)
                                                              gTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGc                                                                                                                                           >  2:359082/1‑94 (MQ=255)
                                                                ttACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCt                                                                                 >  2:113030/1‑149 (MQ=255)
                                                                  aCGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCtt                                                                                <  1:200160/148‑1 (MQ=255)
                                                                  aCGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTc                                                                               >  6:175018/1‑149 (MQ=255)
                                                                   cGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTc                                                                               <  3:23794/148‑1 (MQ=255)
                                                                       gTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGAcgc                                                                          <  8:221531/149‑1 (MQ=255)
                                                                            ttCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCgccgg                                                                     >  3:239142/1‑149 (MQ=255)
                                                                                  cACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGAc                                                                >  1:15910/1‑148 (MQ=255)
                                                                                  cACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGAc                                                                <  2:15910/148‑1 (MQ=255)
                                                                                            gcggcCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCaa                                                        <  2:80255/146‑1 (MQ=255)
                                                                                                   tCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCATCCGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGTCGACCTTACCAACGCCCCCgg                                               <  1:113030/149‑1 (MQ=255)
                                                                                                      ccGACGAGCT‑GGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCgccggc                                                                    >  8:316908/1‑123 (MQ=255)
                                                                                                      ccGACGAGCT‑GGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCgccggc                                                                    <  7:316908/123‑1 (MQ=255)
                                                                                                           gaGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCAca                                      >  6:164083/1‑148 (MQ=255)
                                                                                                                   ccGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGcc                              >  7:125899/1‑149 (MQ=255)
                                                                                                                     gTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCg                            <  8:125899/149‑1 (MQ=255)
                                                                                                                      tGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGa                           <  5:175018/149‑1 (MQ=255)
                                                                                                                         tCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGATc                         >  4:374042/1‑148 (MQ=255)
                                                                                                                          cgcgAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGATCaa                       >  6:329493/1‑149 (MQ=255)
                                                                                                                           gcgAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGATCAAc                      <  3:365193/149‑1 (MQ=255)
                                                                                                                                   caacgcgCCGCTGGCGTCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGATCAACCAGCTggg              >  2:89684/4‑149 (MQ=255)
                                                                                                                                     gcgcGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGATCAACCAGCTGGGCa            >  5:342073/1‑149 (MQ=255)
                                                                                                                                       gcgcCGCCCGCGGCGCGTTGCACACCGTCGACGACCTGGGCTTTGCCAT‑TGCCGTCCAGGTGACCTGCCGTCGCACCTTCGACGCGCCGGCCGACCATCCCCACGCCCCCGGTAGCGCACCGCCGCCCCCCAGCCACCAGCAGGGCaag          >  4:100627/1‑147 (MQ=255)
                                                                                                                                             ccAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGATCAACCAGCTGGGCATTGCCCCg    <  4:239142/149‑1 (MQ=255)
                                                                                                                                               aGCGGCGCGTTGCCCACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGCCCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGATCAACCAGCTGGGCATTGCCACGGa  >  7:317164/1‑149 (MQ=255)
                                                                                                                                               aGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAAc                                                       >  5:17731/1‑96 (MQ=255)
                                                                                                                                               aGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAAc                                                       <  6:17731/96‑1 (MQ=255)
                                                                                                                                                gcggcgCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCCGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGATCAACCAGCTGGGCATAGCCCCGGa  >  5:322618/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCCGCGGCGACGACCCTGGCCTGCCGCCAGCGCTGGGGCCGACCCCATCGCTGCGCGAGACCGTTTACGGCGTCGCTTCGAGCACCGATCTGGCGGCCATCGCCGACGAGCTGGGCCGTGATCGCGAAGTGCGGCGCGACGCCAGCGGCGCGTTGCACACCTTCGACGACCTGGGCTTTGCCAT‑TGCCTTCCAGGTGACCTGCCGTCGCCCCTTCGACGCGCCGGCCGACCTTACCAACGCCCCCGGTAGCGCACCGCAGCGCCCGATCAACCAGCTGGGCATTGCCCCGGA  >  NC_002947/3626930‑3627221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑