Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,686,147 C→G 5.3% C185W (TGC→TGG)  PP_3251 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,686,1470CG 5.3% 250.0 / 3.1 75C185W (TGC→TGG) PP_3251hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (35/36);  new base G (2/2);  total (37/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

GAAGAGCTCGGCAACGGTGATCCACGCTGCAACCATGTGCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACGGGCAGTATTTTCAGCTGCATGTGACCATCGACAATGCCGCCAGCG  >  NC_002947/3686001‑3686295
                                                                                                                                                   |                                                                                                                                                    
gAAGAGCTCGGCAACGGTGATCCACGCTGCAACCATGTGCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGc                                                                                                                                                      <  5:343195/147‑1 (MQ=255)
     gCTCGGCAACGGTGATCCACGCTGCAACCATGTGCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTc                                                                                                                                               <  2:257967/149‑1 (MQ=255)
      cTCGGCAACGGTGATCCACGCTGCAACCATGTGCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCa                                                                                                                                              >  7:191817/1‑149 (MQ=255)
       tCGGCAACGGTGATCCACGCTGCAACCATGTGCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCAc                                                                                                                                             >  7:170466/1‑149 (MQ=255)
        cGGCAACGGTGATCCACGCTGCAACCATGTGCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCAcc                                                                                                                                            <  3:193302/149‑1 (MQ=255)
           cAACGGTGATCCACGCTGCAACCATGTGCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGc                                                                                                                                         >  1:271981/1‑149 (MQ=255)
           cAACGGTGATCCACGCTGCAACCATGTGCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGc                                                                                                                                         <  7:145595/149‑1 (MQ=255)
              cGGTGATCCACGCTGCAACCATGTGCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGc                                                                                                                                         >  8:199305/1‑146 (MQ=255)
                    tCCACGCTGCAACCATGTGCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAggtgg                                                                                                                                >  7:223939/1‑149 (MQ=255)
                     ccACGCTGCAACCATGTGCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTG‑CCTGGGGCAGCACTGCGACACTCACCTGCCAGAggtg                                                                                                                                 >  3:184600/1‑146 (MQ=255)
                     ccACGCTGCAACCATGTGCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTG‑CCTGGGGCAGCACTGCGACACTCACCTGCCAGAggtg                                                                                                                                 <  4:184600/146‑1 (MQ=255)
                        cGCTGCAACCATGTGCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTgg                                                                                                                            <  8:191817/149‑1 (MQ=255)
                             cAACCATGTGCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACa                                                                                                                       <  2:22803/149‑1 (MQ=255)
                               aCCATGTGCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAAc                                                                                                                     <  8:170466/149‑1 (MQ=255)
                                  aTGTGCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTc                                                                                                                  <  8:211576/149‑1 (MQ=255)
                                   tgtgCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCg                                                                                                                 <  5:51305/149‑1 (MQ=255)
                                   tgtgCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCg                                                                                                                 <  5:183106/149‑1 (MQ=255)
                                     tgCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCgg                                                                                                                <  3:146867/148‑1 (MQ=255)
                                       cTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGGCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTa                                                                                                             <  6:75182/149‑1 (MQ=255)
                                       cTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTa                                                                                                             >  5:234797/1‑149 (MQ=255)
                                              aTCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGt                                                                                                               >  6:6391/1‑140 (MQ=255)
                                              aTCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGt                                                                                                               <  5:6391/140‑1 (MQ=255)
                                               tCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGagcagc                                                                                                     >  8:4237/1‑149 (MQ=255)
                                                  gcgcCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAggt                                                                                                                                  <  5:252179/117‑1 (MQ=255)
                                                  gcgcCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAggt                                                                                                                                  >  6:252179/1‑117 (MQ=255)
                                                  gcgcCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCAc                                                                                                  <  7:199305/149‑1 (MQ=255)
                                                  gcgcCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCAc                                                                                                  <  6:234797/149‑1 (MQ=255)
                                                    gcCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCg                                                                                                >  3:289566/1‑149 (MQ=255)
                                                        gcttgctCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGagcagc                                                                                                     <  7:333855/140‑1 (MQ=255)
                                                        gcttgctCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGagcagc                                                                                                     >  8:333855/1‑140 (MQ=255)
                                                        gcttgctCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTg                                                                                             >  3:287795/1‑148 (MQ=255)
                                                         cttgctCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGc                                                                                            <  4:287795/148‑1 (MQ=255)
                                                                gCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATctgct                                                                                     >  5:297065/1‑148 (MQ=255)
                                                                 ccTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGa                                                                                   <  4:15566/149‑1 (MQ=255)
                                                                    gggTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGAtaa                                                                                >  8:74746/1‑147 (MQ=255)
                                                                      gTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATctgct                                                                                     <  7:326864/142‑1 (MQ=255)
                                                                      gTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATctgct                                                                                     >  8:326864/1‑142 (MQ=255)
                                                                      gTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATcacc                                                                              <  8:251660/149‑1 (MQ=255)
                                                                           gTAGAGGCCATGCCCCTGGATGACACGCGGTATCTGTCAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACg                                                                           >  1:254171/1‑148 (MQ=255)
                                                                           gTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTa                                                                         <  2:360956/149‑1 (MQ=255)
                                                                             agagGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGGGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACg                                                                       >  7:103446/1‑149 (MQ=255)
                                                                             agagGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGGGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACg                                                                       >  7:108804/1‑149 (MQ=255)
                                                                              gagGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATc                                                                                 >  6:111376/1‑138 (MQ=255)
                                                                              gagGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATc                                                                                 <  5:111376/138‑1 (MQ=255)
                                                                                 gCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCt                                                                   <  6:347192/149‑1 (MQ=255)
                                                                                   cATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTgg                                                                 <  6:297065/149‑1 (MQ=255)
                                                                                      gCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTTCCCTGGGGCAGCACTGCGACACTCa                                                                                                                                              <  8:341237/69‑1 (MQ=255)
                                                                                      gCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCa                                                                                                                                              >  7:341237/1‑69 (MQ=255)
                                                                                         ccTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCa                                                                                           >  4:201854/1‑117 (MQ=255)
                                                                                             gATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGGGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTgg                                                        <  8:103446/148‑1 (MQ=255)
                                                                                             gATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGGGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTgg                                                        <  8:108804/148‑1 (MQ=255)
                                                                                             gATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTgg                                                        <  7:74746/148‑1 (MQ=255)
                                                                                              aTGTCACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGc                                                      <  5:313728/149‑1 (MQ=255)
                                                                                                 acacGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCAt                                                    <  4:289566/148‑1 (MQ=255)
                                                                                                   acGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGa                                                 >  3:144886/1‑149 (MQ=255)
                                                                                                   acGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTACCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGa                                                 >  8:83539/1‑149 (MQ=255)
                                                                                                     gcgGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACg                                               >  2:139106/1‑149 (MQ=255)
                                                                                                      cgGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCg                                                  >  5:201160/1‑145 (MQ=255)
                                                                                                      cgGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCg                                                  <  6:201160/145‑1 (MQ=255)
                                                                                                        gTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTACCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACGGGc                                            <  7:83539/149‑1 (MQ=255)
                                                                                                                cAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCCCGTACGAGCTGGCCGAACTGGGCATCGACGGGCAGTAttt                                     >  6:211310/1‑149 (MQ=255)
                                                                                                                cAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACGGGCAGTAttt                                     >  3:116799/1‑149 (MQ=255)
                                                                                                                 aaGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATc                                                                                         >  7:127395/1‑96 (MQ=255)
                                                                                                                 aaGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATc                                                                                         <  8:127395/96‑1 (MQ=255)
                                                                                                                  aGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACGGGCAGTAtttt                                    >  2:312259/1‑148 (MQ=255)
                                                                                                                    gggCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCAGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACGGGCAGTATTTTCAg                                 <  8:223939/149‑1 (MQ=255)
                                                                                                                     ggCCCTGCAACTTGACCTGGGGCAGCACTGCGACTCTCACCTGCCAGAGGTGGTTGGCTACAAGCTCGGTTATGAGCAGCCAGCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACGGGCAGTATTTTCAGc                                >  8:18777/1‑149 (MQ=255)
                                                                                                                          tGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACGGGCAGTATTTTCAGCTGCAt                           >  4:105220/1‑149 (MQ=255)
                                                                                                                             aaCTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACGGGCAGTATTTTCAGCTGCAtgt                         >  2:365071/1‑148 (MQ=255)
                                                                                                                              aCTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACGGGCAGTATTTTCAGCTGCATGTGa                       <  2:254171/149‑1 (MQ=255)
                                                                                                                                   cccTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACGGGCAGTATTTTCAGCTGCATGTGACCATc                  <  1:365071/149‑1 (MQ=255)
                                                                                                                                    ccTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACGGGCAGTATTTTCAGCTGCATGTGACCATc                  >  8:155721/1‑148 (MQ=255)
                                                                                                                                    ccTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACGGGCAGTATTTTCAGCTGCATGTGACCATc                  >  6:232144/1‑148 (MQ=255)
                                                                                                                                     cTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACGGGCAGTATTTTCAGCTGCATGTGACCATCGa                >  7:143779/1‑149 (MQ=255)
                                                                                                                                         ggCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACGGGCAGTATTTTCAGCTGCATGTGACCATCGACAAt            <  7:4237/149‑1 (MQ=255)
                                                                                                                                           cagcaCTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACGGGCAGTATTTTCAGCTGCATGTGACAATCGACAATgc          >  5:299588/1‑149 (MQ=255)
                                                                                                                                                   cGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACGGGCAGTATTTTCAGCTGCATGTGCCCATCGACAATGCCGCCAGCg  >  2:260601/1‑149 (MQ=255)
                                                                                                                                                   cGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACGGGCAGTATTTTCAGCTGCATGTGACCATCGACAATGCCGCCAGCg  <  3:105220/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GAAGAGCTCGGCAACGGTGATCCACGCTGCAACCATGTGCTGATCTATCAGCGCCTGCTTGCTCGCCTGGGTTGCGTAGAGGCCATGCCCCTGGATGACACGCGGTATCTG‑CAAGGGGCCCTGCAACTTGCCCTGGGGCAGCACTGCGACACTCACCTGCCAGAGGTGGTTGGCTACAACCTCGGTTATGAGCAGCCACCGCTGCATCTGCTGATCACCACGTACGAGCTGGCCGAACTGGGCATCGACGGGCAGTATTTTCAGCTGCATGTGACCATCGACAATGCCGCCAGCG  >  NC_002947/3686001‑3686295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑