Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 308,213 G→A 6.0% intergenic (+1228/‑641) hslO → / → PP_0254 molecular chaperone HslO/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947308,2130GA 6.0% 196.6 / 2.1 69intergenic (+1228/‑641)hslO/PP_0254molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (34/28);  new base A (2/2);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01

CAGAACAGCCGTGCGGCCTACCCGCTGGAGCACGTTGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCGGCTGGCGAGTACGCCGAGCTGCTGATCAAGC  >  NC_002947/308067‑308361
                                                                                                                                                  |                                                                                                                                                    
cAGAACAGCCGTGCGGCCTACCCGCTGGAGCACGTTGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAggcggc                                                                                                                                                    >  7:12106/1‑149 (MQ=255)
 aGAACAGCCGTGCGGCCTACCCGCTGGAGCACGTTGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGcc                                                                                                                                                   <  2:193865/149‑1 (MQ=255)
     cAGCCGTGCGGCCTACCCGCTGGAGCACGTTGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTAc                                                                                                                                                >  3:351218/1‑148 (MQ=255)
      aGCCGTGCGGCCTACCCGCTGGAGCACGTTGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCa                                                                                                                                              >  7:355191/1‑149 (MQ=255)
          gTGCGGCCTACCCGCTGGAGCACGTTGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTAcc                                                                                                                                               <  3:97396/144‑1 (MQ=255)
          gTGCGGCCTACCCGCTGGAGCACGTTGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTAcc                                                                                                                                               >  4:97396/1‑144 (MQ=255)
                  tACCCGCTGGAGCACGTTGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCAGCCt                                                                                                                                                  >  6:140055/1‑133 (MQ=255)
                  tACCCGCTGGAGCACGTTGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCAGCCt                                                                                                                                                  <  5:134212/133‑1 (MQ=255)
                  tACCCGCTGGAGCACGTTGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCAGCCt                                                                                                                                                  >  6:134212/1‑133 (MQ=255)
                  tACCCGCTGGAGCACGTTGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCAGCCt                                                                                                                                                  <  5:140055/133‑1 (MQ=255)
                     cctatGGAGCACGTTGCCAAGCGTTCGGAAGAGAACCTGGGAGGCGAGCCTAACGAAGTGATCTTCCTCACCTGCGACATGACAGGTGTTCTGCATCCGGTTTAGATCCTTAACAACGAGCAGGCGGCCTACCACTTCCTTTCCGGCt                                                                                                                                >  2:11497/5‑148 (MQ=255)
                     ccGCTGGAGCACGTTGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTa                                                                                                                               >  1:68368/1‑149 (MQ=255)
                     ccGCTGGACCACGAAGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGACCCTAACCCAGTGATCATCCTCACCAGCGACCTGACAGGACTTCTGCATCCGGTTTCGATCCTGAACAACGAGCAGGCGCCCTACCACTTCCTCTCCGGCTa                                                                                                                               >  2:5540/1‑149 (MQ=255)
                        cTGGAGCACGTTGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTacac                                                                                                                            <  2:68368/149‑1 (MQ=255)
                            aGCACGTTGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACcgcg                                                                                                                        >  7:301285/1‑149 (MQ=255)
                              cACGTTGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTc                                                                                                                                          <  8:64829/129‑1 (MQ=255)
                              cACGTTGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTc                                                                                                                                          >  7:64829/1‑129 (MQ=255)
                                 gTTGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGt                                                                                                                   >  1:379807/1‑149 (MQ=255)
                                    gCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCg                                                                                                                 <  2:379807/148‑1 (MQ=255)
                                     ccAATCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCTGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGt                                                                                                               <  1:11497/149‑1 (MQ=255)
                                     ccAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTTAACAACGAGCAGGCTGCCTACCACTTCCTGTCCGGGTACACCGCGCTGGTCGGt                                                                                                               <  1:5540/149‑1 (MQ=255)
                                         gCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCa                                                                                                           >  3:22942/1‑149 (MQ=255)
                                         gCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCa                                                                                                           <  4:8429/149‑1 (MQ=255)
                                            ttCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTg                                                                                                        <  4:351218/149‑1 (MQ=255)
                                               ggAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCa                                                                                                           >  7:136799/1‑143 (MQ=255)
                                               ggAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCa                                                                                                           <  8:136799/143‑1 (MQ=255)
                                               ggAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGaaa                                                                                                     >  8:229671/1‑149 (MQ=255)
                                                  aGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATgg                                                                                                  <  8:301285/149‑1 (MQ=255)
                                                  aGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATgg                                                                                                  >  4:123455/1‑149 (MQ=255)
                                                            ggcggcGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTggcgg                                                                                        >  4:343056/1‑149 (MQ=255)
                                                              cggcgAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTggcggcg                                                                                      <  3:149711/149‑1 (MQ=255)
                                                                      cTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAg                                                                              <  3:343056/149‑1 (MQ=255)
                                                                                 aTCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACt                                                                                                         <  6:47383/111‑1 (MQ=255)
                                                                                 aTCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACt                                                                                                         >  5:47383/1‑111 (MQ=255)
                                                                                   cTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCCAGTCGCCCTTCGCCa                                                                 >  8:266221/1‑149 (MQ=255)
                                                                                    ttCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTAc                                                                                                                                                >  3:284551/1‑69 (MQ=255)
                                                                                    ttCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTAc                                                                                                                                                <  4:284551/69‑1 (MQ=255)
                                                                                    ttCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCAc                                                                >  7:277103/1‑149 (MQ=255)
                                                                                     tCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCAc                                                                <  6:154251/148‑1 (MQ=255)
                                                                                           ccTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTc                                                         >  4:108099/1‑149 (MQ=255)
                                                                                             tGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGt                                                                                                                   >  2:372405/1‑89 (MQ=255)
                                                                                             tGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGt                                                                                                                   <  1:372405/89‑1 (MQ=255)
                                                                                                  ccTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCAc                                                  <  7:162587/149‑1 (MQ=255)
                                                                                                   cTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCAcc                                                 >  8:150487/1‑149 (MQ=255)
                                                                                                    tGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCg                                                        >  5:317404/1‑141 (MQ=255)
                                                                                                    tGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCg                                                        <  6:317404/141‑1 (MQ=255)
                                                                                                          gtgtTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGttcttc                                          <  7:150487/149‑1 (MQ=255)
                                                                                                            gtTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTccc                                        >  6:132174/1‑149 (MQ=255)
                                                                                                             ttCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCcg                                       >  2:51270/1‑149 (MQ=255)
                                                                                                               cTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCcgcg                                     >  8:34355/1‑149 (MQ=255)
                                                                                                               cTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCcgcg                                     >  2:246043/1‑149 (MQ=255)
                                                                                                                   cTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTAc                                                                                                                                                >  1:316419/1‑38 (MQ=255)
                                                                                                                   cTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTAc                                                                                                                                                <  2:316419/38‑1 (MQ=255)
                                                                                                                    tCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCgg                                >  7:304232/1‑149 (MQ=255)
                                                                                                                     ccGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCATTCTTCCCGCGTCCGGc                               >  8:85407/1‑149 (MQ=255)
                                                                                                                       ggTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCGGCTg                             <  8:12106/149‑1 (MQ=255)
                                                                                                                        gTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCGGCTgg                            <  7:266221/149‑1 (MQ=255)
                                                                                                                         tttCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCGGCTGGc                           <  4:22942/149‑1 (MQ=255)
                                                                                                                         tttCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCGGCTGGc                           >  1:223635/1‑149 (MQ=255)
                                                                                                                          ttCGATCCTGAACAACGAGCAGGCCGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCGGCTGGc                           >  8:99348/1‑148 (MQ=255)
                                                                                                                              aTCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCGGCTGGCGAGTa                      >  8:217653/1‑149 (MQ=255)
                                                                                                                               tCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCGGCt                              >  4:31082/1‑140 (MQ=255)
                                                                                                                               tCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCGGCt                              <  3:31082/140‑1 (MQ=255)
                                                                                                                                 cTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCGGCTGGCGAGTACg                    <  1:51270/148‑1 (MQ=255)
                                                                                                                                 cTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCGGCTGGCGAGTACGc                   >  1:158767/1‑149 (MQ=255)
                                                                                                                                    aacaacGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCGGCTGGCGAGTACGCCGa                <  1:246043/149‑1 (MQ=255)
                                                                                                                                     acaacGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCGGCTGGCGAGTACGCCGAg               <  7:99348/149‑1 (MQ=255)
                                                                                                                                         cGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCGGCTGGCGAGTACGCCGAgctgc           <  2:158767/149‑1 (MQ=255)
                                                                                                                                             cAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCa                                                                                                           >  6:341390/1‑49 (MQ=255)
                                                                                                                                             cAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCa                                                                                                           <  5:341390/49‑1 (MQ=255)
                                                                                                                                                gcggcCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTccc                                        >  4:28431/1‑113 (MQ=255)
                                                                                                                                                gcggcCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTccc                                        <  3:28431/113‑1 (MQ=255)
                                                                                                                                                  ggcCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCGGCTGGCGAGTACGCCGAGCTGCTGATCAAGc  <  3:123455/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CAGAACAGCCGTGCGGCCTACCCGCTGGAGCACGTTGCCAAGCGTTCGGAAGCGAACCTGGGCGGCGAGCCTAACGCAGTGATCTTCCTCACCTGCGACCTGACAGGTGTTCTGCCTCCGGTTTCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCGCTGGTCGGTTCCACTGAAATGGGTTCTGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCGGCTGGCGAGTACGCCGAGCTGCTGATCAAGC  >  NC_002947/308067‑308361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑