Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,718,088 C→G 12.4% E169Q (GAG→CAG)  paaG ← 2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,718,0880CG12.4% 206.2 / 16.5 72E169Q (GAG→CAG) paaG2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase
Reads supporting (aligned to +/- strand):  ref base C (29/34);  new base G (6/3);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 3.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCC  >  NC_002947/3717942‑3718233
                                                                                                                                                  |                                                                                                                                                 
gAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCg                                                                                                                                                  <  5:173837/148‑1 (MQ=255)
      gcgcTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCa                                                                                                                                           >  5:174841/1‑149 (MQ=255)
            gATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCTCAGCATGGc                                                                                                                                       >  1:283026/1‑147 (MQ=255)
                aGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCATGGCCCGGGCCAAGGCGCTGGCCATGCTGGGCGAgcgc                                                                                                                                 >  8:23374/1‑149 (MQ=37)
                    tCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGTTCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCa                                                                                                                                     >  7:118153/1‑141 (MQ=255)
                    tCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGTTCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCa                                                                                                                                     <  8:118153/141‑1 (MQ=255)
                    tCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTGGCCATGCTGGGCGAGCGCCtt                                                                                                                              >  2:346375/1‑148 (MQ=255)
                    tCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTGGCCATGCTGGGCGAGCGCCtt                                                                                                                              <  1:346375/148‑1 (MQ=255)
                    tCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGATGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTg                                                                                                                             <  8:196081/149‑1 (MQ=255)
                        gCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGccc                                                                                                                         >  8:357707/1‑149 (MQ=255)
                           gTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCggg                                                                                                                      >  2:156938/1‑149 (MQ=255)
                           gTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCggg                                                                                                                      <  6:201469/149‑1 (MQ=255)
                              ggTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGcc                                                                                                                    <  4:162448/148‑1 (MQ=255)
                               gTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGc                                                                                                                     <  5:205454/146‑1 (MQ=255)
                               gTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGc                                                                                                                     >  6:205454/1‑146 (MQ=255)
                                 gggctgggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGTCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCATGGCCCGGGCCATGc                                                                                                                <  5:55695/149‑1 (MQ=255)
                                    ctgggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGa                                                                                                             <  7:357707/149‑1 (MQ=255)
                                     tgggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGAc                                                                                                            >  1:80918/1‑149 (MQ=255)
                                        gcggcgAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCaa                                                                                                         <  1:156938/149‑1 (MQ=255)
                                           gcgAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCATGGCCCGGGCCAAGGCGCTGGCCATGCTGGGCGAGCTCCTTGGCGCCGAACAGGCCGAGCaaag                                                                                                       >  7:132645/1‑146 (MQ=17)
                                               ggTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCgg                                                                                                   >  2:65131/1‑148 (MQ=255)
                                                     gggcgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTg                                                                                                                             >  8:216674/1‑116 (MQ=255)
                                                     gggcgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTg                                                                                                                             <  7:216674/116‑1 (MQ=255)
                                                        cgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCaa                                                                                         <  6:174841/149‑1 (MQ=255)
                                                               gAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTAccgc                                                                                  <  1:65131/149‑1 (MQ=255)
                                                                  ggCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTAccgccg                                                                                >  3:163676/1‑148 (MQ=255)
                                                                    cTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAg                                                                             >  1:166181/1‑149 (MQ=255)
                                                                     ttCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGt                                                                            >  2:273537/1‑149 (MQ=255)
                                                                     ttCATCACGAAGCGCTGCATCATCCACCACGCTGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCTCTCGCCCATCATGGCCAGCGCCTTGGcc                                                                                                                          <  8:186835/103‑1 (MQ=255)
                                                                     ttCATCACGAAGCGCTGCATCATCAACCACGCGGTAGATAACCCACCATTGCTCGGCCTGTTCGGCGCCAAGGAGCTCGCCCAGCATGGCCAGCGCCTTGGcc                                                                                                                          >  7:186835/1‑103 (MQ=255)
                                                                      tcatcaCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCAt                                                                                                                  <  2:364212/110‑1 (MQ=255)
                                                                      tcatcaCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCAt                                                                                                                  >  1:364212/1‑110 (MQ=255)
                                                                       catcaCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGt                                                                            <  1:214284/147‑1 (MQ=255)
                                                                       catcaCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTcc                                                                          >  3:191371/1‑149 (MQ=255)
                                                                            cGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCAc                                                                     <  4:338594/149‑1 (MQ=255)
                                                                             gCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCAcc                                                                    >  8:4276/1‑149 (MQ=255)
                                                                                 cgcTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCTCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGc                                                                <  2:283026/149‑1 (MQ=255)
                                                                                 cgcTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGc                                                                <  7:258764/149‑1 (MQ=255)
                                                                                      catcatCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATc                                                           <  2:80918/149‑1 (MQ=255)
                                                                                          atcCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTg                                                        <  2:166181/148‑1 (MQ=255)
                                                                                          atcCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTg                                                        <  4:191371/148‑1 (MQ=255)
                                                                                          atcCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTg                                                        <  6:254211/148‑1 (MQ=255)
                                                                                               ccacGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAg                                                  <  1:273537/149‑1 (MQ=255)
                                                                                                 acGCGGTAGATCAGCCCCCATTGCTCCGCCTGTTCGGCGCCTAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGc                                                <  5:325872/149‑1 (MQ=255)
                                                                                                      gTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGaa                                                   >  1:16376/1‑141 (MQ=255)
                                                                                                      gTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGaa                                                   <  2:16376/141‑1 (MQ=255)
                                                                                                               cccccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTgg                                  <  7:230105/149‑1 (MQ=255)
                                                                                                               cccccATTGCTCGGCCTGTTCCGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTgg                                  >  2:107365/1‑149 (MQ=255)
                                                                                                                ccccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCGTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGc                                 <  6:115481/149‑1 (MQ=255)
                                                                                                                           ggCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgcgggcgg                      >  8:120641/1‑149 (MQ=255)
                                                                                                                            gCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGTCCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGggcggc                     <  7:263465/149‑1 (MQ=255)
                                                                                                                            gCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGggcggc                     <  3:186479/149‑1 (MQ=255)
                                                                                                                            gCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGggcggc                     >  8:263465/1‑149 (MQ=255)
                                                                                                                               tGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgcgggcg                       <  5:307647/144‑1 (MQ=255)
                                                                                                                               tGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgcgggcg                       >  6:307647/1‑144 (MQ=255)
                                                                                                                                gTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgcgggcgg                      <  8:313403/144‑1 (MQ=255)
                                                                                                                                gTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGggcggcagat                 >  7:313403/1‑145 (MQ=255)
                                                                                                                                 ttCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGc                 <  7:4276/148‑1 (MQ=255)
                                                                                                                                    ggCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCAc               >  1:155095/1‑147 (MQ=255)
                                                                                                                                       gcCAAGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGt          >  6:170109/1‑149 (MQ=39)
                                                                                                                                       gcCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGggcggc                     <  7:344537/138‑1 (MQ=255)
                                                                                                                                       gcCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGggcggc                     >  8:344537/1‑138 (MQ=255)
                                                                                                                                         cAAGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCGCCGAACAGGCCGAGCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAg            <  7:326480/145‑1 (MQ=25)
                                                                                                                                         cAAGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCGCCGAACAGGCCGAGCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAg            >  8:326480/1‑145 (MQ=25)
                                                                                                                                         cAAGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTgg                                            <  1:163486/113‑1 (MQ=38)
                                                                                                                                         cAAGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTgg                                            >  2:163486/1‑113 (MQ=38)
                                                                                                                                          aaGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCg        >  8:80167/1‑148 (MQ=255)
                                                                                                                                            ggCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAg     >  2:174061/1‑149 (MQ=255)
                                                                                                                                            ggCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAg     >  1:359975/1‑149 (MQ=255)
                                                                                                                                             gcgcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAgg    >  8:38983/1‑149 (MQ=255)
                                                                                                                                               gcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGcc  <  2:359975/149‑1 (MQ=255)
                                                                                                                                               gcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGc   >  2:331135/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCC  >  NC_002947/3717942‑3718233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑