Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 317,711 C→G 8.2% E74Q (GAA→CAA)  dctD‑I ← C4‑dicarboxylate transport transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947317,7110CG 8.2% 199.0 / 7.6 73E74Q (GAA→CAA) dctD‑IC4‑dicarboxylate transport transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (36/31);  new base G (2/4);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.65e-01

CGCACGCTGTCGAGCAGGGCGTCTGTGGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAGGGTCTGGCTCAAGG  >  NC_002947/317563‑317857
                                                                                                                                                    |                                                                                                                                                  
cgcACGCTGTCGAGCAGGGCGTCTGTGGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTc                                                                                                                                                    <  7:308671/149‑1 (MQ=255)
 gcACGCTGTCGAGCAGGGCGTCTGTGGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCTGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTcc                                                                                                                                                   <  1:172047/149‑1 (MQ=255)
     gCTGTCGAGCAGGGCGTCTGTGGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGc                                                                                                                                                >  7:251869/1‑148 (MQ=255)
         tCGAGCAGGGCGTCTGTGGCAAAGGGTTTTTCCAGGAAGTCATGAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAAc                                                                                                                                             <  3:313433/147‑1 (MQ=255)
             gCAGGGCGTCTGTGGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCATCACCGGCAGTTCGCTGTCGCGGCC‑TGCAGTTGCTGGAACAACTGCacgg                                                                                                                                      <  3:276689/149‑4 (MQ=38)
             gCAGGGCGTCTGTGGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCC‑TGCAGTTGCTGGAACAACTGCacgg                                                                                                                                      <  3:183086/149‑4 (MQ=38)
             gCAGGGCGTCTGTGGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCC‑TGCAGTTGCTGGAACAACTGCacgg                                                                                                                                      <  3:20930/149‑4 (MQ=38)
                    gTCTGTGGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGa                                                                                                                                >  2:210546/1‑149 (MQ=255)
                     tCTGTGGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGAt                                                                                                                               >  7:252653/1‑149 (MQ=255)
                         tgGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGccc                                                                                                                           >  5:231606/1‑149 (MQ=255)
                         tgGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGccc                                                                                                                           >  5:282210/1‑149 (MQ=255)
                           gCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCg                                                                                                                          <  1:14433/148‑1 (MQ=255)
                            cAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCgg                                                                                                                         >  3:258074/1‑148 (MQ=255)
                             aaaGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCa                                                                                                                                    <  6:42329/136‑1 (MQ=255)
                             aaaGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCa                                                                                                                                    >  5:42329/1‑136 (MQ=255)
                             aaaGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGACTGGAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCCTCGATGCACGGCa                                                                                                                       >  5:269194/1‑149 (MQ=39)
                               aGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCACCTGCAGGCCATCGATGCCCGGCATg                                                                                                                     >  5:200297/1‑149 (MQ=255)
                                gggTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGc                                                                                                                    <  8:251869/149‑1 (MQ=255)
                                  gTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCAt                                                                                                                                   >  7:2175/1‑132 (MQ=255)
                                  gTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCAt                                                                                                                                   <  8:2175/132‑1 (MQ=255)
                                        ccAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCg                                                                                                            >  5:76752/1‑149 (MQ=255)
                                         cAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAAt                                                                                                                <  6:341001/144‑1 (MQ=255)
                                         cAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAAt                                                                                                                >  5:341001/1‑144 (MQ=255)
                                         cAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGc                                                                                                           <  6:286410/149‑1 (MQ=255)
                                         cAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGc                                                                                                           >  7:174209/1‑149 (MQ=255)
                                          aGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCt                                                                                                          <  4:89549/149‑1 (MQ=255)
                                                 cATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGaccacc                                                                                                   >  5:360685/1‑149 (MQ=255)
                                                  aTAACCCCCGCCGCGCATCGCCTGAAAAGCCAGCGGCACATCGCCATGGCCGGTAATCAGAAGCACCGGCAGTTCGCTGTCCCGCCCGTACACTTGTTCCAGCAACTGAAGGACATCGATGCACGGCATGAGAATGTCGCTGAcaacca                                                                                                  >  8:234949/1‑149 (MQ=255)
                                                   tAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGATCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACcaccac                                                                                                 <  8:63981/149‑1 (MQ=255)
                                                     aGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGc                                                                                               >  8:174098/1‑149 (MQ=255)
                                                      gCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCa                                                                                                                                         <  6:374577/106‑1 (MQ=255)
                                                      gCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCa                                                                                                                                         >  5:374577/1‑106 (MQ=255)
                                                          cGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGc                                                                                          >  2:202769/1‑149 (MQ=255)
                                                             cgcgcATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAg                                                                                       <  6:200297/149‑1 (MQ=255)
                                                                 cATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCaa                                                                                                                                              <  7:251040/90‑1 (MQ=255)
                                                                 cATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCaa                                                                                                                                              >  8:251040/1‑90 (MQ=255)
                                                                 cATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGAACACCACGCCGGGCCAGTCgg                                                                                   >  2:93470/1‑149 (MQ=255)
                                                                  aTCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGc                                                                                  <  2:360196/149‑1 (MQ=255)
                                                                  aTCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGc                                                                                  >  5:381234/1‑149 (MQ=255)
                                                                   tCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGcc                                                                                 >  4:95428/1‑149 (MQ=255)
                                                                   tCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGcc                                                                                 >  4:100817/1‑149 (MQ=255)
                                                                      ccTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCg                                                                              <  3:120848/149‑1 (MQ=255)
                                                                          cACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGAt                                                                            <  6:282210/147‑1 (MQ=255)
                                                                          cACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGAt                                                                            <  8:252653/147‑1 (MQ=255)
                                                                          cACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGAt                                                                            <  6:231606/147‑1 (MQ=255)
                                                                          cACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCCTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATAc                                                                          >  2:358005/1‑149 (MQ=255)
                                                                             cGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCt                                                                       >  4:316063/1‑149 (MQ=255)
                                                                              gCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGGTGTTGCAGCCACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCGGCCCCCCCCCCCGGGCCAGTCGGCCGCGATACGCTc                                                                      >  3:194934/1‑149 (MQ=255)
                                                                                  gCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGTAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGcc                                                                  >  4:198456/1‑149 (MQ=255)
                                                                                     gCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAgg                                                               <  8:174209/149‑1 (MQ=255)
                                                                                           cGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACAACGCCGGGCCAGTCCGCCTCGATACGCTCGGCAAGCCCTTgg                                                         >  6:92816/1‑149 (MQ=255)
                                                                                            gCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTgg                                                         <  3:316063/148‑1 (MQ=255)
                                                                                              cATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCGAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTggg                                                        <  6:269194/147‑1 (MQ=255)
                                                                                                  gCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCg                                                   <  4:258074/148‑1 (MQ=255)
                                                                                                          tCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCAGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAgcgc                                           >  6:335246/1‑148 (MQ=255)
                                                                                                            agcagcACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGa                                         <  6:360685/148‑1 (MQ=255)
                                                                                                                gcaCCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTAc                                     >  4:373091/1‑148 (MQ=255)
                                                                                                                 caCCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCt                                   >  3:217898/1‑149 (MQ=255)
                                                                                                                  aCCGTCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTTGGCTTCGGCCAGCGCGACTACCtt                                  <  7:234949/149‑1 (MQ=255)
                                                                                                                  aCCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCtt                                  <  4:194934/149‑1 (MQ=255)
                                                                                                                  aCCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGAATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCtt                                  >  6:105489/1‑149 (MQ=255)
                                                                                                                    cGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCtt                                  >  5:22615/1‑147 (MQ=255)
                                                                                                                     ggCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTc                                                                               <  6:5435/101‑1 (MQ=255)
                                                                                                                     ggCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTc                                                                               >  5:5435/1‑101 (MQ=255)
                                                                                                                              cTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCTAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAg                      <  1:93470/149‑1 (MQ=255)
                                                                                                                              cTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAg                      <  3:95428/149‑1 (MQ=255)
                                                                                                                              cTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAg                      <  3:100817/149‑1 (MQ=255)
                                                                                                                                gTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGt                    >  6:270958/1‑149 (MQ=255)
                                                                                                                                   gatggcctGCAGTTGCTGGAACAACTGCACGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGcc                        <  2:245714/135‑1 (MQ=255)
                                                                                                                                   gatggcctGCAGTTGCTGGAACAACTGCACGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGcc                        >  1:245714/8‑142 (MQ=255)
                                                                                                                                           gCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAGGGTCTgg         >  5:163544/1‑149 (MQ=255)
                                                                                                                                            cAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAGGGTCTgg         <  6:76752/148‑1 (MQ=255)
                                                                                                                                                 gttCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGa                                         >  2:254985/1‑111 (MQ=255)
                                                                                                                                                 gttCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAGGGTCTGGCTCaa    <  1:358005/148‑1 (MQ=255)
                                                                                                                                                  ttCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAGGGTCTGGCTCAAgg  <  7:174098/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGCACGCTGTCGAGCAGGGCGTCTGTGGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCAGGCCATCGATGCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAGGGTCTGGCTCAAGG  >  NC_002947/317563‑317857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑