Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,762,942	A→T	11.1%	N62I (AAC→ATC)	PP_3326 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,762,942	0	A	T	11.1%	163.7 / 9.6	63	N62I (AAC→ATC)	PP_3326	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (28/28);  new base T (4/3);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

ATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCAT  >  NC_002947/3762797‑3763087                                                                                                                                                   |                                                                                                                                                     aTGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAAc                                                                                                                                                     <  6:26367/147‑1 (MQ=255) aTGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACa                                                                                                                                                    <  6:303119/148‑1 (MQ=255)    gTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGAt                                                                                                                                                <  1:166914/149‑1 (MQ=255)    gTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGAt                                                                                                                                                >  6:36057/1‑149 (MQ=255)       aGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATc‑‑cc                                                                                                                                           <  4:109928/149‑1 (MQ=255)         cGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCgg                                                                                                                                         >  3:61459/1‑149 (MQ=255)         cGTAAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGAAc‑‑cccg                                                                                                                                         >  1:344304/1‑147 (MQ=255)          gTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACCGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGCTCGACCGTGACCGGGATCATGTTC‑‑CCgg                                                                                                                                         <  4:227686/149‑1 (MQ=255)             aaCGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGCGACCGGGAACATGATC‑‑CCGGTCAc                                                                                                                                     >  7:305682/1‑149 (MQ=255)                  aGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTCTACGGCCACGGCGCCGAGGATAACGACTACAAGGCGGAAAAGATCAAGCGCTCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTc                                                                                                                                 >  7:256404/1‑148 (MQ=255)                               cTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGa                                                                                                                  >  1:220716/1‑149 (MQ=255)                                tgtgGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGCTCGACCGTGACCGGGATCATGTTC‑‑CCgg                                                                                                                                         <  2:326333/127‑1 (MQ=39)                                tgtgGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGCTCGACCGTGACCGGGATCATGTTC‑‑CCgg                                                                                                                                         >  1:326333/1‑127 (MQ=39)                                tgtgGACAGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGCTCGACCGTGACCGGGATCATGTTC‑‑CCgct                                                                                                                                        >  5:199724/1‑126 (MQ=38)                                    gACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATg                                                                                                             <  2:220716/149‑1 (MQ=255)                                        gaggagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGACGGATCATGCCGt                                                                                                         >  1:191266/1‑149 (MQ=255)                                         aggagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGtg                                                                                                        <  5:157355/149‑1 (MQ=255)                                           gagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGCACATGATC‑‑CCGGTCACGGGCGAGCG‑CCTGCCGGATCATGCCGagct                                                                                                      >  2:177124/1‑149 (MQ=255)                                            agCGACGTGGAAATATCGAGGCCGGGTACGGCCTCGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGtgctg                                                                                                     <  2:344304/149‑1 (MQ=255)                                              cGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGtgctgc                                                                                                    >  1:44794/1‑148 (MQ=255)                                                aCGTGGCAATATCGAGGCCGTGTACGGCCAAGGCGCCGAGGATAACGCCTACAAGGAGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGAACGGGAAc                                                                                                                                                     >  7:265049/1‑100 (MQ=255)                                                  gTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCa                                                                                               <  5:36057/149‑1 (MQ=255)                                                   tGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCAc                                                                                              >  7:213594/1‑149 (MQ=255)                                                        atatCGAGGCCGTGTACGGCCTCGGCGCCGAGGTTAACGGCTAGAAGGCGGAAAAGATCAAGCGCGCCTGGGAGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGACCATGCCGTGCTGCAACGACTTAcc                                                                                         <  5:234523/149‑1 (MQ=255)                                                            cGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGc                                                                                     >  3:144085/1‑149 (MQ=255)                                                             gAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGc                                                                                     <  4:277695/148‑1 (MQ=255)                                                                         ggCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGc                                                                        >  3:336397/1‑149 (MQ=255)                                                                         ggCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGc                                                                        <  8:124526/149‑1 (MQ=255)                                                                           ccACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCg                                                                      >  2:23909/1‑149 (MQ=255)                                                                           ccACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCg                                                                      >  5:387725/1‑149 (MQ=255)                                                                            cACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGt                                                                     <  4:88985/149‑1 (MQ=255)                                                                             aCGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGtg                                                                    <  2:194986/149‑1 (MQ=255)                                                                                   ccGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGctgctg                                                              <  4:144085/149‑1 (MQ=255)                                                                                     gAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTg                                                                         <  5:219600/136‑1 (MQ=255)                                                                                     gAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTg                                                                         >  6:219600/1‑136 (MQ=255)                                                                                          tAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCg                                                                      <  7:167677/134‑1 (MQ=255)                                                                                          tAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCg                                                                      >  8:167677/1‑134 (MQ=255)                                                                                           aaCGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCAGGCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGCCAAc                                                        >  1:113378/1‑149 (MQ=255)                                                                                            aCGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACgg                                                      <  2:44794/148‑1 (MQ=255)                                                                                                 tACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTg                                                >  1:295581/1‑149 (MQ=255)                                                                                                   cAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTg                                                <  7:308463/147‑1 (MQ=255)                                                                                                             aaGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGccc                                    >  2:96966/1‑149 (MQ=255)                                                                                                             aaGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGccc                                    <  4:61459/149‑1 (MQ=255)                                                                                                             aaGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGACGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGccc                                    <  2:191266/149‑1 (MQ=255)                                                                                                                aTCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGa                                 >  1:229539/1‑149 (MQ=255)                                                                                                                   aaGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACaa                              <  6:218476/149‑1 (MQ=255)                                                                                                                   aaGCGCGCCTGGGCGTTCGA‑CGCGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACaa                              <  8:305682/149‑1 (MQ=255)                                                                                                                       gcgcCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACg                                                       <  3:337707/120‑1 (MQ=255)                                                                                                                       gcgcCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACg                                                       <  3:218623/120‑1 (MQ=255)                                                                                                                       gcgcCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACg                                                       >  4:218623/1‑120 (MQ=255)                                                                                                                       gcgcCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACg                                                       >  4:337707/1‑120 (MQ=255)                                                                                                                          ccTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCt                                                                                   >  3:24949/1‑89 (MQ=255)                                                                                                                          ccTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCt                                                                                   <  4:24949/89‑1 (MQ=255)                                                                                                                          ccTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATgg                       <  8:213594/149‑1 (MQ=255)                                                                                                                           cTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATggg                      >  3:267441/1‑149 (MQ=255)                                                                                                                           cTGGGCGGTAGA‑CGGTACCGGGAACATGATC‑‑CCGGGCACGGGCGAGCG‑CCTGGCGGAGCATGCCGTGCTGCAACGACTTACCCCGCCTGCGGCAATTGCCGGGCGGCTGGACAACGGTCCGTGGTAACAGCGCCCGGACAATCAATggg                      <  2:194606/149‑1 (MQ=255)                                                                                                                            tGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGt                     >  4:323501/1‑149 (MQ=255)                                                                                                                                      accGTGACCGGGATCATGTTC‑‑CCGGTCAC‑GTCGAACGCCCAGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAgg             >  8:337444/3‑148 (MQ=37)                                                                                                                                          tGACCGGGATCATGTTC‑‑CCGGTCAC‑GTCGAACG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACa                               >  2:143440/1‑125 (MQ=39)                                                                                                                                          tGACCGGGATCATGTTC‑‑CCGGTCAC‑GTCGAACG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACa                               <  1:143440/125‑1 (MQ=39)                                                                                                                                          tGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCg         >  7:236779/1‑148 (MQ=255)                                                                                                                                              cGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCAccc    <  4:336397/149‑1 (MQ=255)                                                                                                                                                ggAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCAt  >  1:54390/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     ATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATC‑‑CCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCAT  >  NC_002947/3762797‑3763087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑