Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,775,330 T→G 5.3% A49A (GCA→GCC)  PP_3336 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,775,3300TG 5.3% 243.1 / 2.6 75A49A (GCA→GCC) PP_3336hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (33/38);  new base G (2/2);  total (35/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

GCAGCAGCAATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTTCTCTCCAAC  >  NC_002947/3775183‑3775477
                                                                                                                                                   |                                                                                                                                                   
gcagcagcaATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATg                                                                                                                                                    >  8:193981/1‑149 (MQ=255)
  agcagcaATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGCCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCTTGGGCAATGc                                                                                                                                                   >  8:207408/1‑148 (MQ=255)
   gcagcaATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCa                                                                                                                                                 <  7:98268/149‑1 (MQ=255)
    cagcaATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAg                                                                                                                                                <  4:200330/149‑1 (MQ=255)
        aaTGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTAcc                                                                                                                                            >  2:188813/1‑149 (MQ=255)
         aTGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCATTGCCCATGGCCAGTAccc                                                                                                                                           <  3:151716/149‑2 (MQ=255)
             ccGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGag                                                                                                                                       <  7:193981/149‑1 (MQ=255)
             ccGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGag                                                                                                                                       <  7:339323/149‑1 (MQ=255)
             ccGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGag                                                                                                                                       <  2:317722/149‑1 (MQ=255)
                    cGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATg                                                                                                                                <  6:351170/149‑1 (MQ=255)
                    cGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATg                                                                                                                                <  3:226846/149‑1 (MQ=255)
                          tCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAagc                                                                                                                          <  4:311982/149‑1 (MQ=255)
                            ttCGGGATAAGTTTGCCGTCCTTGTCCAGCCTGGCCCGCTGTACGCCGCCCGCCCAGCAATAACTGCCCCCCAGCAACTGCGCCAGGCTCAGGTCGACCAGGGTAAAGGCCAAGGGCAATGCCACTACCTTGAGCAGCATGGCAagcag                                                                                                                        >  7:82984/1‑149 (MQ=255)
                             tCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAagcagc                                                                                                                       >  1:173117/1‑149 (MQ=255)
                               gggCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAgcg                                                                                                                      <  5:362149/148‑1 (MQ=255)
                                     aGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGa                                                                                                                <  4:148230/148‑1 (MQ=255)
                                       tttGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGAcc                                                                                                              >  1:215710/1‑148 (MQ=255)
                                       tttGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGAcc                                                                                                              >  5:44722/1‑148 (MQ=255)
                                         tGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAagcag                                                                                                                        <  8:86524/136‑1 (MQ=255)
                                         tGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAagcag                                                                                                                        >  7:86524/1‑136 (MQ=255)
                                           ccGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCagag                                                                                                         <  7:222725/149‑1 (MQ=255)
                                           ccGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCaga                                                                                                          <  4:240633/148‑1 (MQ=255)
                                                 ttGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATc                                                                                                   >  6:345855/1‑149 (MQ=255)
                                                    tCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATcc                                                                                                  >  1:223648/1‑147 (MQ=255)
                                                     ccAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGGTATGCCAg                                                                                               >  7:139519/1‑149 (MQ=255)
                                                          aTGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAgcg                                                                                                                      <  1:159651/121‑1 (MQ=255)
                                                          aTGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAgcg                                                                                                                      >  2:159651/1‑121 (MQ=255)
                                                              ccGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGtgcgtgcg                                                                                      >  3:112805/1‑149 (MQ=255)
                                                              ccGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGtgcgtgcg                                                                                      >  3:101810/1‑149 (MQ=255)
                                                               cGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTgcgc                                                                                     <  4:115466/149‑1 (MQ=255)
                                                               cGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTgcgc                                                                                     >  6:233183/1‑149 (MQ=255)
                                                                 gCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTgcgcgc                                                                                   <  4:101810/149‑1 (MQ=255)
                                                                        gccgccCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCg                                                                             >  5:335023/1‑148 (MQ=255)
                                                                          cgccCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAag                                                                                                                           <  5:19416/100‑1 (MQ=255)
                                                                          cgccCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAag                                                                                                                           >  6:19416/1‑100 (MQ=255)
                                                                              cgacgaGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGgc                                                                      <  6:93222/149‑1 (MQ=255)
                                                                                acgaGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCTTGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGgcgc                                                                    <  7:207408/149‑1 (MQ=255)
                                                                                acgaGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCa                                                                                                <  3:53495/121‑1 (MQ=255)
                                                                                acgaGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCa                                                                                                >  4:53495/1‑121 (MQ=255)
                                                                                    gCAATAACTGCCCGCCAGCATCTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGt                                                                >  6:292232/1‑149 (MQ=255)
                                                                                    gCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGt                                                                >  4:70056/1‑149 (MQ=255)
                                                                                     cAATAACTGCGCGACAGCAACTTAGCCAGGCTCCGGTCGCCCAGGGTACTGGCCCTGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGGAGCCCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCAtttt                                                               <  8:82984/149‑1 (MQ=255)
                                                                                      aaTAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGtt                                                               >  5:231130/1‑148 (MQ=255)
                                                                                            tGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCt                                                        >  3:316815/1‑149 (MQ=255)
                                                                                              cccGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCg                                                      >  5:147849/1‑149 (MQ=255)
                                                                                                cGCCAGCAACTGCGCCAGGCTCAGGGCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGcc                                                    <  1:188813/149‑1 (MQ=255)
                                                                                                 gCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGc                                                     >  5:258158/1‑147 (MQ=255)
                                                                                                  ccAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAg                                                  <  5:345855/149‑1 (MQ=255)
                                                                                                       aaCTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGTGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAgc                                             >  5:247801/1‑149 (MQ=255)
                                                                                                         cTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAgcgc                                           >  7:299633/1‑149 (MQ=255)
                                                                                                          tGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAg                                                                                 <  4:80746/110‑1 (MQ=255)
                                                                                                                 ggCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTgg                                   <  7:344544/149‑1 (MQ=255)
                                                                                                                  gCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTg                                    >  2:126922/1‑147 (MQ=255)
                                                                                                                   cTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGgag                                 <  4:112805/149‑1 (MQ=255)
                                                                                                                      aGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGgaga                                <  2:173117/147‑1 (MQ=255)
                                                                                                                       ggTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTc                             <  5:292232/149‑1 (MQ=255)
                                                                                                                              cAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGt                      >  6:322139/1‑149 (MQ=255)
                                                                                                                              cAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGt                      <  6:258158/149‑1 (MQ=255)
                                                                                                                                 ggTACTGGCATTGCCCATGGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTc                             <  8:331634/139‑1 (MQ=255)
                                                                                                                                 ggTACTGGCATTGCCCATGGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCAt                   >  1:355507/1‑149 (MQ=255)
                                                                                                                                 ggTACTGGCATTGCCCATGGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGAATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTc                             >  7:331634/1‑139 (MQ=255)
                                                                                                                                   tACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCAt                   >  8:138222/1‑147 (MQ=255)
                                                                                                                                      tGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAagtagc                                                                                                                       <  7:188254/44‑1 (MQ=255)
                                                                                                                                      tGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAagtagc                                                                                                                       >  8:188254/1‑44 (MQ=255)
                                                                                                                                        gCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGc             <  6:147849/148‑1 (MQ=255)
                                                                                                                                           aTGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGTGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTtc          >  1:148317/1‑148 (MQ=255)
                                                                                                                                           aTGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGa                 >  3:236721/1‑141 (MQ=255)
                                                                                                                                           aTGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGa                 <  4:236721/141‑1 (MQ=255)
                                                                                                                                           aTGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTtct         <  6:335023/149‑1 (MQ=255)
                                                                                                                                              ggCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTtctctc      <  3:70056/149‑1 (MQ=255)
                                                                                                                                              ggCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTtctct       <  4:316815/148‑1 (MQ=255)
                                                                                                                                                 aaTGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTTCTCTCCaa   <  5:322139/149‑1 (MQ=255)
                                                                                                                                                 aaTGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTTCTCTCCaa   >  3:366637/1‑149 (MQ=255)
                                                                                                                                                 aaTGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATATTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTTCTCTCCaa   <  6:44722/149‑1 (MQ=255)
                                                                                                                                                  aTGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTTCTCTCCAac  <  5:233183/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCAGCAGCAATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTTCTCTCCAAC  >  NC_002947/3775183‑3775477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑