Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,788,398 G→C 7.9% Y79* (TAC→TAG)  PP_3350 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,788,3980GC 7.9% 192.4 / 6.7 63Y79* (TAC→TAG) PP_3350hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (30/28);  new base C (2/3);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

ACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTCCCCGGCCGG  >  NC_002947/3788254‑3788545
                                                                                                                                                |                                                                                                                                                   
aCGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCggagaa                                                                                                                                                 >  1:238439/1‑145 (MQ=255)
   ggTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTTCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCggg                                                                                                                                              <  3:275457/149‑1 (MQ=255)
    gTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGc                                                                                                                                             <  3:131330/149‑1 (MQ=255)
       ttCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTc                                                                                                                                          <  5:219095/149‑1 (MQ=255)
        tctcGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGACGCATCGGCCCGGTAGCGGGCCt                                                                                                                                           >  5:128623/1‑147 (MQ=255)
          tcGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTACTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGc                                                                                                                                       >  5:305358/1‑149 (MQ=255)
            gACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGccc                                                                                                                                     >  2:108005/1‑149 (MQ=255)
            gACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGCTACCGGGccgatgc                                                                                                                                       <  4:124113/147‑6 (MQ=255)
             aCCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGcccc                                                                                                                                    <  7:169557/149‑1 (MQ=255)
               cTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCt                                                                                                                                           <  5:326679/140‑1 (MQ=255)
               cTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCt                                                                                                                                           >  6:326679/1‑140 (MQ=255)
               cTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGa                                                                                                                                  <  7:40319/149‑1 (MQ=255)
               cTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGCTACCGGGccga                                                                                                                                          <  5:112844/141‑3 (MQ=255)
               cTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGCTACCGGGccga                                                                                                                                          >  6:112844/1‑139 (MQ=255)
                  gACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGtt                                                                                                                               >  4:224211/1‑149 (MQ=255)
                  gACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGtt                                                                                                                               >  6:68687/1‑149 (MQ=255)
                    cGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGc                                                                                                                             <  3:196618/149‑1 (MQ=255)
                         gTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCgg                                                                                                                                               <  1:43168/126‑1 (MQ=255)
                         gTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGACCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCgg                                                                                                                                               >  2:43168/1‑126 (MQ=255)
                             gCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGcc                                                                                                                    >  7:125487/1‑149 (MQ=255)
                                  gCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGActc                                                                                                               <  1:129348/149‑1 (MQ=255)
                                          gAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCTGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGcc                                                                                                       >  4:105030/1‑149 (MQ=255)
                                                gTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATgg                                                                                                  >  3:196475/1‑148 (MQ=255)
                                                gTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATgg                                                                                                  >  2:214393/1‑148 (MQ=255)
                                                gTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCAGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATgg                                                                                                  >  6:168375/1‑148 (MQ=255)
                                                 tGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTa                                                                                                <  8:125487/149‑1 (MQ=255)
                                                         ggCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAgg                                                                                         <  5:168375/148‑1 (MQ=255)
                                                          gCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGc                                                                                                                                             >  6:67427/1‑95 (MQ=255)
                                                          gCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGc                                                                                                                                             <  5:67427/95‑1 (MQ=255)
                                                               ggCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCg                                                                                   >  6:24851/1‑148 (MQ=255)
                                                                 ccTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGaaa                                                                                <  2:109302/149‑1 (MQ=255)
                                                                  cTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAg                                                                               <  5:68687/149‑1 (MQ=255)
                                                                  cTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAg                                                                               <  6:124495/149‑1 (MQ=255)
                                                                     cAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAgg                                                                              <  4:196475/147‑1 (MQ=255)
                                                                               tgcAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCtt                                                                  >  8:103439/1‑149 (MQ=255)
                                                                               tgcAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCtt                                                                  >  8:267855/1‑149 (MQ=255)
                                                                                gcAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGa                                                                                                                                  >  4:284853/1‑84 (MQ=255)
                                                                                gcAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGa                                                                                                                                  <  3:284853/84‑1 (MQ=255)
                                                                                 cAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTgg                                                                >  5:214859/1‑149 (MQ=255)
                                                                                       ggAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGTGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCgggg                                                           <  1:75356/148‑1 (MQ=255)
                                                                                        gAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGgtc                                                         >  2:68623/1‑149 (MQ=255)
                                                                                           tcgtcgTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGgtcgtc                                                      >  8:313079/1‑149 (MQ=255)
                                                                                               cgtTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCgaga                                                  <  7:103439/149‑1 (MQ=255)
                                                                                                     gCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCgg                                            <  5:24851/149‑1 (MQ=255)
                                                                                                      cccGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAggg                                                                             >  8:160367/1‑115 (MQ=255)
                                                                                                      cccGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAggg                                                                             <  7:160367/115‑1 (MQ=255)
                                                                                                      cccGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCgg                                            >  2:306498/1‑148 (MQ=255)
                                                                                                       ccGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTa                                          <  7:267855/149‑1 (MQ=255)
                                                                                                          cgcAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTc                                       <  3:224211/149‑1 (MQ=255)
                                                                                                               gCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGa                                  >  3:75510/1‑149 (MQ=255)
                                                                                                                 cAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAg                                                                                                                                        <  8:311359/45‑1 (MQ=255)
                                                                                                                 cAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAg                                                                                                                                        >  7:311359/1‑45 (MQ=255)
                                                                                                                 cAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATc                                >  2:265460/1‑149 (MQ=255)
                                                                                                                      aagGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGcc                                                                    >  4:20517/1‑108 (MQ=255)
                                                                                                                         ggTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAgg                        <  6:305358/149‑1 (MQ=255)
                                                                                                                              gTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGccc                   >  6:244572/1‑149 (MQ=255)
                                                                                                                                  gAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGGAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGa                                  <  8:112548/130‑1 (MQ=255)
                                                                                                                                  gAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGa                                  >  7:112548/1‑130 (MQ=255)
                                                                                                                                   aaGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCgg                                            <  6:48017/119‑1 (MQ=255)
                                                                                                                                       tgaggCCCGCTACCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTcc          >  7:346415/4‑149 (MQ=255)
                                                                                                                                       aTCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTcc          >  3:115061/1‑149 (MQ=255)
                                                                                                                                       aTCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTcc          <  6:214859/149‑1 (MQ=255)
                                                                                                                                         aggCCCGCTACCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTcc          <  8:346415/146‑1 (MQ=255)
                                                                                                                                          ggCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTCCccg       >  7:156952/1‑149 (MQ=255)
                                                                                                                                               ggTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTCCccggccgg  <  5:244572/149‑1 (MQ=255)
                                                                                                                                                gTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCt                                     <  3:91585/113‑1 (MQ=255)
                                                                                                                                                gTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCt                                     >  4:91585/1‑113 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
ACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTCCCCGGCCGG  >  NC_002947/3788254‑3788545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑