Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,796,426 T→C 6.3% T10A (ACC→GCC)  fcs ← feruloyl‑CoA‑synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,796,4260TC 6.3% 180.7 / 2.5 63T10A (ACC→GCC) fcsferuloyl‑CoA‑synthetase
Reads supporting (aligned to +/- strand):  ref base T (33/26);  new base C (2/2);  total (35/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

ACCAGGCGCTCGAGCAGGCGCGCCGGCAGCGGCGCCAGTGGCTCGACAGGTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGTCTGGGCCTGCCGCGGCTAGATGGGATAGTGACGCGGGCCGTG  >  NC_002947/3796284‑3796571
                                                                                                                                              |                                                                                                                                                 
aCCAGGCGCTCGAGCAGGCGCGCCGGCAGCGGCGCCAGTGGCTCGACAGGTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGAccc                                                                                                                                             >  8:227460/1‑149 (MQ=255)
 ccAGGCGCTCGAGCAGGCGCGCCGGCAGCGGCGCCAGTGGCTCGACAGGTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCt                                                                                                                                            >  1:207763/1‑149 (MQ=255)
    ggCGCTCGAGCAGGCGCGCCGGCAGCGGCGCCAGTAGCTCGACAGGTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAg                                                                                                                                         >  1:247495/1‑149 (MQ=255)
      cgcTCGAGCAGGCGCGCCGGCAGCGGCGCCAGTGGCTCGACAGGTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGTCAGGGTCGACCGACCCTggcca                                                                                                                                       <  8:292017/149‑5 (MQ=255)
           gAGCAGGCGCGCCGGCAGCGGCGCCAGTGGCTCGACAGGTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCtt                                                                                                                                  >  6:338087/1‑149 (MQ=255)
                       cGGCAGCGGCGCCAGTGGCTCGACAGGTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACg                                                                                                                       >  5:282114/1‑148 (MQ=255)
                       cGGCAGCGGCGCCAGTGGCTCGACAGGTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACg                                                                                                                       >  3:54513/1‑148 (MQ=255)
                       cGGCAGCGGCGCCAGTGGCTCGACAGGTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGc                                                                                                                      <  4:54513/149‑1 (MQ=255)
                              ggcgCCAGTGGCTCGACAGGTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGCGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGc                                                                                                               <  5:269433/149‑1 (MQ=255)
                                  ccAGTGGCTCGACAGGTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGATGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCct                                                                                                           <  5:374565/149‑1 (MQ=255)
                                    aGTGGCTCGACAGGTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCcttg                                                                                                         >  3:151069/1‑149 (MQ=255)
                                          tCGACAGGTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTc                                                                                                             >  6:21348/1‑139 (MQ=255)
                                          tCGACAGGTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTc                                                                                                             <  5:21348/139‑1 (MQ=255)
                                          tCGACAGGTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCcttgttcttg                                                                                                   <  5:338087/149‑1 (MQ=255)
                                            gACAGGTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCcttgttcttg                                                                                                   <  6:282114/147‑1 (MQ=255)
                                             aCAGGTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCttgttg                                                                                                >  6:76970/1‑149 (MQ=255)
                                                 gTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGt                                                                                                                  <  2:41235/127‑1 (MQ=255)
                                                 gTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGt                                                                                                                  >  1:41235/1‑127 (MQ=255)
                                                  ttGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTCCTTGTTGTACAt                                                                                           <  2:247495/149‑1 (MQ=255)
                                                    gCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTAc                                                                                             >  8:217123/1‑145 (MQ=255)
                                                    gCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTAc                                                                                             <  7:217123/145‑1 (MQ=255)
                                                    gCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATc                                                                                          <  2:207763/148‑1 (MQ=255)
                                                         cgcAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTgccg                                                                                    >  8:199784/1‑149 (MQ=255)
                                                               aCGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGccc                                                                              >  4:103618/1‑149 (MQ=255)
                                                                  tcgtcgACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCcttgttcttgt                                                                                                  >  8:222233/1‑126 (MQ=255)
                                                                  tcgtcgACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCcttgttcttgt                                                                                                  <  7:222233/126‑1 (MQ=255)
                                                                    gtcgACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCcttgttc                                                                                                      <  5:166722/120‑1 (MQ=255)
                                                                    gtcgACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCcttgttc                                                                                                      >  6:166722/1‑120 (MQ=255)
                                                                    gtcgACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGatat                                                                         >  1:81465/1‑149 (MQ=255)
                                                                       gACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGAc                                                                      >  2:241591/1‑149 (MQ=255)
                                                                        aCGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACg                                                                     >  6:263622/1‑149 (MQ=255)
                                                                        aCGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACg                                                                     >  2:376943/1‑149 (MQ=255)
                                                                            gactgacCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGggc                                                                 >  5:322805/1‑149 (MQ=255)
                                                                                   cTGCACCTGGGGATGCCCGATGGCCACCTGGGGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGt                                                           >  7:74410/1‑148 (MQ=255)
                                                                                     gCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCAt                                                        >  5:184964/1‑149 (MQ=255)
                                                                                        ccTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCtt                                                     <  7:199784/149‑1 (MQ=255)
                                                                                            gggATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTGCGTAATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCg                                                 <  1:118751/149‑1 (MQ=255)
                                                                                              gATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGa                                               <  3:103618/149‑1 (MQ=255)
                                                                                              gATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGa                                               <  6:322805/149‑1 (MQ=255)
                                                                                               aTGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAg                                              <  2:331846/149‑1 (MQ=255)
                                                                                                 gCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGt                                            >  1:71365/1‑149 (MQ=255)
                                                                                                   ccGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTcc                                                                                                            >  3:141336/1‑83 (MQ=255)
                                                                                                   ccGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTcc                                                                                                            <  4:141336/83‑1 (MQ=255)
                                                                                                    cGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGTCTg                                         >  3:240174/1‑149 (MQ=255)
                                                                                                     gATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGTCTgg                                        <  6:184964/149‑1 (MQ=255)
                                                                                                          ccACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGTCTGGGCCTg                                   >  2:331845/1‑149 (MQ=255)
                                                                                                           cACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACgggg                                                                  >  8:153034/1‑117 (MQ=255)
                                                                                                           cACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACgggg                                                                  <  7:153034/117‑1 (MQ=255)
                                                                                                            aCCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGTCTGGGCCTGcc                                 >  7:152605/1‑149 (MQ=255)
                                                                                                             ccTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCt                                                                                                              >  7:8637/1‑71 (MQ=255)
                                                                                                             ccTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCt                                                                                                              <  8:8637/71‑1 (MQ=255)
                                                                                                                          cgGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGTCTGGGCCTGCCGCGGCTAGATGGGa                   <  5:76970/149‑1 (MQ=255)
                                                                                                                           ggACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGTCTGGGCCTGCCGCGGCTAGATGGGa                   >  6:136165/1‑148 (MQ=255)
                                                                                                                            gACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGTCTGGGCCTGCCGCGGCTAGATGGGATa                 <  5:263622/149‑1 (MQ=255)
                                                                                                                              cGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGTCTGGGCCTGCCGCGGCTAGATGGGATAGt               <  8:152605/149‑1 (MQ=255)
                                                                                                                              cGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGTCTGGGCCTGCCGCGGCTAGATGGGATAGt               <  1:241591/149‑1 (MQ=255)
                                                                                                                                ccgctcAGGGTCGACCGACCCTGGGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGTCTGGGCCTGCCGCGGCTAGATGGGATAGTGa             >  3:232839/6‑149 (MQ=255)
                                                                                                                                   gCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGTCTGGGCCTGCCGCGGCTAGATGGGATAGTGAcgc          <  1:331845/149‑1 (MQ=255)
                                                                                                                                    tcAGGGTCGACCGACCCTGAGCGGGCTTGGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGTCTgg                                        >  1:212330/2‑118 (MQ=255)
                                                                                                                                    tcAGGGTCGACCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGTCTgg                                        <  2:212330/117‑1 (MQ=255)
                                                                                                                                    ccAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGTCTGGGCCTGCCGCGGCTAGATGGGATAGTGAcgcg         >  8:212412/1‑149 (MQ=255)
                                                                                                                                      aGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGTCTGGGCCTGCCGCGGCTAGATGGGATAGTGACGCggg       >  1:282198/1‑149 (MQ=255)
                                                                                                                                       gggTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGGCGGAGGTCTGGGGCGGCAGCGGCTAGATGGGATAGTGACGCGTGc      >  8:333695/1‑149 (MQ=255)
                                                                                                                                           aggtcgTCCCTGAGGGGGCTTCGTTATTCAGGCAGGTTGCTCCTTGTTCTTGTTGTTCATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGTCTGGGCCTGCCGCGGCTAGATGGGATAGTGACGCGGGCCGTg  <  4:240174/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
ACCAGGCGCTCGAGCAGGCGCGCCGGCAGCGGCGCCAGTGGCTCGACAGGTTGCATGCGCAGCACGTCGTCGACGTGACTGACCTGCACCTGGGGATGCCCGATGGCCACCTGGCGGTAGCGCGGACGTTGGCCAGGGTCGGTCGACCCTGAGCGGGCTTCGTTATTCACGCAGGTTGCTCCTTGTTCTTGTTGTACATCCTGCCGCCGCCCGATATGACGGGGCGGCGGTCATCTTGCCGGAGGTCTGGGCCTGCCGCGGCTAGATGGGATAGTGACGCGGGCCGTG  >  NC_002947/3796284‑3796571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑