Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,829,899 T→C 8.9% Q494R (CAG→CGG) ‡ PP_3383 ← gluconate 2‑dehydrogenase flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,829,8990TC 8.9% 156.4 / 5.2 56Q494R (CAG→CGG) ‡PP_3383gluconate 2‑dehydrogenase flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (24/27);  new base C (2/3);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-01

GCAGGTAGCGGTTCAGTGCACTGGTCTTGGGGTCAGTGCCCATGATCGCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGGGTCCAGGTCCAGGTAATTGCCACGGTAGGACTGGT  >  NC_002947/3829756‑3830046
                                                                                                                                               |                                                                                                                                                    
gCAGGTAGCGGTTCAGTGCACTGGTCTTGGGGTCAGTGCCCATGATCGCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGc                                                                                                                                                  <  3:95294/148‑1 (MQ=255)
   ggTAGCGGTTCAGTGCACTGGTCTTGGGGTCAGTGCCCATGATCGCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATc                                                                                                                                              >  6:376449/1‑149 (MQ=255)
       gCGGTTCAGTGCACTGGTCTTGGGGTCAGTGCCCATGATCGCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCCAGGCGAtgaac                                                                                                                                           <  4:197521/148‑5 (MQ=255)
             cAGTGCACTGGTCTTGGGGTCAGTGCCCATGATCGCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGc                                                                                                                                     <  4:361049/148‑1 (MQ=255)
             cAGTGCACTGGTCTTGGGGTCAGTGCCCATGATCGCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGc                                                                                                                                     <  5:134697/148‑1 (MQ=255)
                 gCACTGGTCTTGGGGTCAGTGCCCATGATCGCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTc                                                                                                                                <  2:371776/149‑1 (MQ=255)
                    cTGGTCTTGGGGTCAGTGCCCATGATCGCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGTCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGAc                                                                                                                             <  1:265002/149‑1 (MQ=255)
                    cTGGTCTTGGGGTCAGTGCCCATGATCGCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGAc                                                                                                                             <  2:205871/149‑1 (MQ=255)
                     tGGTCTTGGGGTCAGTGCCCATGATCGCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGAc                                                                                                                             >  8:32602/1‑148 (MQ=255)
                            ggggTCAGTGCCCATGATCGCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACt                                                                                                                     >  7:258130/1‑149 (MQ=255)
                                tCAGTGCCCATGATGGCCCCAGCGTTCATGTGGGTGGTCTGGTTAGTGGTGTTGTTGAAGTGGTCCTTGACCTGCTTGACAAGCACGTCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGtt                                                                                                                 <  8:258130/149‑1 (MQ=255)
                                tCAGTGCCCATGATCGCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGtt                                                                                                                 >  7:140123/1‑149 (MQ=255)
                                  aGTGCCCATGATCGCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGTTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTc                                                                                                                <  3:114096/148‑1 (MQ=255)
                                     gCCCATGATCGCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCCAGGCGAtgaaccc                                                                                                                                         <  3:82055/120‑7 (MQ=255)
                                     gCCCATGATCGCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCCAGGCGAtgaaccc                                                                                                                                         >  4:82055/1‑114 (MQ=255)
                                       ccATGATCGCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTTTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTg                                                                                                          <  8:69920/149‑1 (MQ=255)
                                            aTCGCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTc                                                                                                     <  8:140123/149‑1 (MQ=255)
                                               gCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGtt                                                                                                  >  3:145725/1‑149 (MQ=255)
                                                 cccACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGttt                                                                                                 >  1:297651/1‑148 (MQ=255)
                                                      cGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGc                                                                                           >  6:358266/1‑149 (MQ=255)
                                                        ttCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCa                                                                                         <  5:358266/149‑1 (MQ=255)
                                                                 gtggtCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTgg                                                                                                                   >  7:7765/1‑114 (MQ=255)
                                                                 gtggtCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTgg                                                                                                                   <  8:7765/114‑1 (MQ=255)
                                                                     tCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATg                                                                            <  2:297651/149‑1 (MQ=255)
                                                                         gTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGc                                                                                           <  4:188863/130‑1 (MQ=255)
                                                                         gTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGc                                                                                           >  3:188863/1‑130 (MQ=255)
                                                                                 tggtgTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑Gc                                                               >  1:230018/1‑149 (MQ=255)
                                                                                          aGTGGGCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAg                                                      <  1:141539/149‑1 (MQ=255)
                                                                                                    gACCTGCCTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGt                                            <  8:243195/149‑1 (MQ=255)
                                                                                                     aCCTGCGTGCCCAGCACGGCAATGGCCTGGGGGTGCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTGTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGt                                            <  8:280736/148‑1 (MQ=255)
                                                                                                       cTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAgg                                         >  4:33943/1‑149 (MQ=255)
                                                                                                        tGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGt                                        >  2:235051/1‑149 (MQ=255)
                                                                                                         gCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGt                                                        >  8:73617/1‑132 (MQ=255)
                                                                                                         gCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGt                                                        <  7:73617/132‑1 (MQ=255)
                                                                                                           ttGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTggg                                     >  5:367417/1‑149 (MQ=255)
                                                                                                              cccAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGggtc                                  <  4:145725/149‑1 (MQ=255)
                                                                                                               ccAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCgg                                             <  6:131936/137‑1 (MQ=255)
                                                                                                               ccAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCgg                                             >  5:131936/1‑137 (MQ=255)
                                                                                                               ccAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGAGGTAGGTGGggtc                                  >  2:38889/1‑148 (MQ=255)
                                                                                                                     cGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGCTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGggtccaggtcc                           <  5:376449/149‑1 (MQ=255)
                                                                                                                        cAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGGGtccaggtccagg                        >  8:112407/1‑149 (MQ=255)
                                                                                                                         aaTGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGGGtccaggtccagg                        >  4:76132/1‑148 (MQ=255)
                                                                                                                             gCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGGGTCCAGGTCCAGGTAAtt                   <  6:367417/149‑1 (MQ=255)
                                                                                                                             gCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGGGTCCAGGTCCAGGTAAt                    <  7:32602/148‑1 (MQ=255)
                                                                                                                               cTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGGGTCCAGGTCCAGGTAATTGc                 <  3:76132/149‑1 (MQ=255)
                                                                                                                                    gcaagaTCGCCCAGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGGGTCCAGGTCCAGGTAATTGCCACgg            >  2:377404/6‑149 (MQ=255)
                                                                                                                                      ttCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGGGTCCAGGTCCAGGTAATTGCCACgg            >  5:13864/1‑147 (MQ=255)
                                                                                                                                         aTCGCCTGGGTGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGTTGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGGGTCCAGGTCCAGGTAATTGCCACGGTAGGa       >  1:343112/1‑149 (MQ=255)
                                                                                                                                          tCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCGCCCTGACCGTAGGCATCGCGGTAGGTGGGGTCCAGGTCCAGGTAAt                    <  1:269010/136‑1 (MQ=255)
                                                                                                                                          tCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGGGTCCAGGTCCAGGTAAt                    >  2:269010/1‑135 (MQ=255)
                                                                                                                                          tCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGGGTCCAGGTCCAGGTAATTGCCACGGTAGGAc      >  1:188922/1‑149 (MQ=255)
                                                                                                                                          tCGCCCAGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGGGTCCAGGTCCAGGTAATTGCCACGGTAGGAc      <  1:377404/149‑1 (MQ=255)
                                                                                                                                            gCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGGGTCCAGGTCCAGGTAATTGCCACGGTAGGACTg    >  6:104709/1‑149 (MQ=255)
                                                                                                                                             ccTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGGGTCCAGGTCCAGGTAATTGCCACGGTAGGACTgg   >  3:50220/1‑149 (MQ=255)
                                                                                                                                             ccTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGGGTCCAGGTCCAGGTAATTGCCACGGTAGGACTgg   <  6:13864/149‑1 (MQ=255)
                                                                                                                                              cTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGGGTCCAGGTCCAGGTAATTGCCACGGTAGGACTGgt  <  7:112407/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
GCAGGTAGCGGTTCAGTGCACTGGTCTTGGGGTCAGTGCCCATGATCGCCCCACCGTTCAGGTGGGTGGTCTGGTAACTGGTGGTGTTGAAGTGGTCCTTGACCTGCTTGCCCAGCACGGCAATGGCCTTGGGGTTCATCGCCTGGGCGATCTTGCTCAGCTTGTCGACCATGAACTGGTTCATCTTGATGTCGTTTTCCTGCCAGTCGAACGTCATGCGCAGCAGCG‑GCTGACCGTAGGCATCGCGGTAGGTGGGGTCCAGGTCCAGGTAATTGCCACGGTAGGACTGGT  >  NC_002947/3829756‑3830046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑