Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,844,916 A→T 7.8% C240S (TGC→AGC) ‡ PP_3394 ← 3‑hydroxy‑3‑methylglutaryl‑CoA lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,844,9160AT 7.8% 150.0 / 2.1 51C240S (TGC→AGC) ‡PP_33943‑hydroxy‑3‑methylglutaryl‑CoA lyase
Reads supporting (aligned to +/- strand):  ref base A (22/25);  new base T (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GCTCGCCCGGTAGCGCTTCGCTTAGTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTG  >  NC_002947/3844772‑3845057
                                                                                                                                                 |                                                                                                                                              
gCTCGCCCGGTAGCGCTTCGCTTAGTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCt                                                                                                                                            >  6:185038/1‑149 (MQ=255)
 cTCGCCCGGTAGCGCTTCGCTTAGTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTc                                                                                                                                           <  2:200266/149‑1 (MQ=255)
    gCCCGGTAGCGCTTCGCTTAGTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTccc                                                                                                                                         <  5:269866/148‑1 (MQ=255)
      ccGGTAGCGCTTCGCTTAGTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAgg                                                                                                                                      <  7:339831/149‑1 (MQ=255)
                       aGTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCTGGCGGGCCTGGGAGGct                                                                                                                       <  6:324093/147‑2 (MQ=255)
                        gTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATc                                                                                                                     >  5:363950/1‑148 (MQ=255)
                                  ggTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCaa                                                                                                          <  5:185038/149‑1 (MQ=255)
                                   gTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAAt                                                                                                         <  4:103264/149‑1 (MQ=255)
                                    tttCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGTAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATg                                                                                                        <  1:155764/149‑1 (MQ=255)
                                    tttCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATg                                                                                                        >  8:301742/1‑149 (MQ=255)
                                         aTTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCctg                                                                                                   >  3:24259/1‑149 (MQ=255)
                                           tAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACTGGATGCCTCCCTGGCGGGCCTGGGAGGCTGCCCGTTcgcc                                                                                                            <  6:187035/138‑1 (MQ=25)
                                           tAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACTGGATGCCTCCCTGGCGGGCCTGGGAGGCTGCCCGTTcgcc                                                                                                            <  6:181666/138‑1 (MQ=25)
                                           tAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACTGGATGCCTCCCTGGCGGGCCTGGGAGGCTGCCCGTTcgcc                                                                                                            >  5:187035/1‑138 (MQ=25)
                                           tAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACTGGATGCCTCCCTGGCGGGCCTGGGAGGCTGCCCGTTcgcc                                                                                                            >  5:181666/1‑138 (MQ=25)
                                            aGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCctgct                                                                                                 <  5:233945/148‑1 (MQ=255)
                                             gcgcAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCAGTGAACACCAGGTCCTCGGTCACGGAATTACCAGACCCGCCAGGGCCGAACGGGCAGCCTCCCAGCCCCGCCAGGCAg                                                                                                                          >  1:269367/1‑123 (MQ=255)
                                             gcgcAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAg                                                                                                                          <  2:269367/122‑1 (MQ=255)
                                             gcgcAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCtgctgc                                                                                               >  3:347303/1‑149 (MQ=255)
                                                cAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCAcc                                                                                            <  4:347303/149‑1 (MQ=255)
                                                   gCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTccc                                                                                                             <  1:57102/129‑1 (MQ=255)
                                                   gCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTccc                                                                                                             >  2:57102/1‑129 (MQ=255)
                                                     aaGTCGATGCCGGTGTCGTAGCCGAGGCTTGGCAGCA‑TGAACACCAGGTCCTGGGGCACGGTATTACCAGACGCGCCAGGGGCTAACGGGCAGCCTCCGAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGccac                                                                                       <  3:69078/149‑1 (MQ=255)
                                                     aaGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGccac                                                                                       >  8:41679/1‑149 (MQ=255)
                                                                ggTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCa                                                                                                                   <  1:271566/110‑1 (MQ=255)
                                                                ggTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCa                                                                                                                   >  2:271566/1‑110 (MQ=255)
                                                                  tgtCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGcc                                                                          <  6:344460/149‑1 (MQ=255)
                                                                    tCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCaa                                                                        <  6:363950/149‑1 (MQ=255)
                                                                          cccATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAaggcca‑g                                                                 >  1:110148/1‑149 (MQ=255)
                                                                           ccATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAaggcca‑gg                                                                >  2:167848/1‑149 (MQ=255)
                                                                            cATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGcc                                                                                                                                <  8:275972/85‑1 (MQ=255)
                                                                            cATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGcc                                                                                                                                >  7:275972/1‑85 (MQ=255)
                                                                               gCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGcccc                                                            >  2:361874/1‑149 (MQ=255)
                                                                                 tttGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCgg                                                          >  4:320579/1‑149 (MQ=255)
                                                                                 tttGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACCCGCCAGGGGCGAACGGGCAGCCTCCCAGCCCCGCCAGGGAGGCATCCAGTACCCCAATCCCCTGCTGCACCGCCACCAGGCTGTTGCCCAAGGCCA‑GGCCCCgg                                                          >  4:282463/1‑149 (MQ=255)
                                                                                   tGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCgg                                                          <  3:320579/147‑1 (MQ=255)
                                                                                       gca‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCa                                                    <  1:167848/149‑1 (MQ=255)
                                                                                           tGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCg                                                           >  5:127780/1‑139 (MQ=255)
                                                                                           tGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCg                                                           <  6:127780/139‑1 (MQ=255)
                                                                                             aaCACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCgg                                                          <  4:249873/138‑1 (MQ=255)
                                                                                             aaCACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCgg                                                          >  3:249873/1‑138 (MQ=255)
                                                                                                       ccTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGcc                                     >  6:261638/1‑149 (MQ=255)
                                                                                                         tCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCtt                                   >  7:169095/1‑149 (MQ=255)
                                                                                                         tCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCtt                                   >  1:59237/1‑149 (MQ=255)
                                                                                                           ggTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTg                                  <  7:301742/148‑1 (MQ=255)
                                                                                                                  gTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTc                          <  7:41679/149‑1 (MQ=255)
                                                                                                                                   aGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTga                                 <  2:20847/125‑1 (MQ=255)
                                                                                                                                     gggCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACg       >  3:82414/1‑149 (MQ=255)
                                                                                                                                          aaCGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTg  <  8:169095/149‑1 (MQ=255)
                                                                                                                                           aCGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCATGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGAc                              <  1:61550/121‑1 (MQ=255)
                                                                                                                                           aCGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGAc                              >  2:61550/1‑120 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
GCTCGCCCGGTAGCGCTTCGCTTAGTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTG  >  NC_002947/3844772‑3845057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑