Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,844,933 A→T 11.7% L234Q (CTG→CAG)  PP_3394 ← 3‑hydroxy‑3‑methylglutaryl‑CoA lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,844,9330AT11.7% 156.7 / 11.3 60L234Q (CTG→CAG) PP_33943‑hydroxy‑3‑methylglutaryl‑CoA lyase
Reads supporting (aligned to +/- strand):  ref base A (27/26);  new base T (3/4);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01

AGTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCATTGCCACCTGGCC  >  NC_002947/3844795‑3845078
                                                                                                                                           |                                                                                                                                                  
aGTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCTGGCGGGCCTGGGAGGct                                                                                                                                            <  6:324093/147‑2 (MQ=255)
 gTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATc                                                                                                                                          >  5:363950/1‑148 (MQ=255)
           ggTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCaa                                                                                                                               <  5:185038/149‑1 (MQ=255)
            gTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAAt                                                                                                                              <  4:103264/149‑1 (MQ=255)
             tttCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGTAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATg                                                                                                                             <  1:155764/149‑1 (MQ=255)
             tttCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATg                                                                                                                             >  8:301742/1‑149 (MQ=255)
                  aTTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCctg                                                                                                                        >  3:24259/1‑149 (MQ=255)
                    tAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACTGGATGCCTCCCTGGCGGGCCTGGGAGGCTGCCCGTTcgcc                                                                                                                                 >  5:187035/1‑138 (MQ=25)
                    tAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACTGGATGCCTCCCTGGCGGGCCTGGGAGGCTGCCCGTTcgcc                                                                                                                                 >  5:181666/1‑138 (MQ=25)
                    tAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACTGGATGCCTCCCTGGCGGGCCTGGGAGGCTGCCCGTTcgcc                                                                                                                                 <  6:181666/138‑1 (MQ=25)
                    tAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACTGGATGCCTCCCTGGCGGGCCTGGGAGGCTGCCCGTTcgcc                                                                                                                                 <  6:187035/138‑1 (MQ=25)
                     aGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCctgct                                                                                                                      <  5:233945/148‑1 (MQ=255)
                      gcgcAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCAGTGAACACCAGGTCCTCGGTCACGGAATTACCAGACCCGCCAGGGCCGAACGGGCAGCCTCCCAGCCCCGCCAGGCAg                                                                                                                                               >  1:269367/1‑123 (MQ=255)
                      gcgcAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAg                                                                                                                                               <  2:269367/122‑1 (MQ=255)
                      gcgcAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCtgctgc                                                                                                                    >  3:347303/1‑149 (MQ=255)
                         cAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCAcc                                                                                                                 <  4:347303/149‑1 (MQ=255)
                            gCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTccc                                                                                                                                  >  2:57102/1‑129 (MQ=255)
                            gCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTccc                                                                                                                                  <  1:57102/129‑1 (MQ=255)
                              aaGTCGATGCCGGTGTCGTAGCCGAGGCTTGGCAGCA‑TGAACACCAGGTCCTGGGGCACGGTATTACCAGACGCGCCAGGGGCTAACGGGCAGCCTCCGAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGccac                                                                                                            <  3:69078/149‑1 (MQ=255)
                              aaGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGccac                                                                                                            >  8:41679/1‑149 (MQ=255)
                                         ggTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCa                                                                                                                                        <  1:271566/110‑1 (MQ=255)
                                         ggTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCa                                                                                                                                        >  2:271566/1‑110 (MQ=255)
                                           tgtCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGcc                                                                                               <  6:344460/149‑1 (MQ=255)
                                             tCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCaa                                                                                             <  6:363950/149‑1 (MQ=255)
                                                   cccATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAaggcca‑g                                                                                      >  1:110148/1‑149 (MQ=255)
                                                    ccATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAaggcca‑gg                                                                                     >  2:167848/1‑149 (MQ=255)
                                                        gCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGcccc                                                                                 >  2:361874/1‑149 (MQ=255)
                                                          tttGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCgg                                                                               >  4:320579/1‑149 (MQ=255)
                                                          tttGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACCCGCCAGGGGCGAACGGGCAGCCTCCCAGCCCCGCCAGGGAGGCATCCAGTACCCCAATCCCCTGCTGCACCGCCACCAGGCTGTTGCCCAAGGCCA‑GGCCCCgg                                                                               >  4:282463/1‑149 (MQ=255)
                                                            tGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCgg                                                                               <  3:320579/147‑1 (MQ=255)
                                                                gca‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCa                                                                         <  1:167848/149‑1 (MQ=255)
                                                                    tGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCg                                                                                <  6:127780/139‑1 (MQ=255)
                                                                    tGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCg                                                                                >  5:127780/1‑139 (MQ=255)
                                                                      aaCACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCgg                                                                               <  4:249873/138‑1 (MQ=255)
                                                                      aaCACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCgg                                                                               >  3:249873/1‑138 (MQ=255)
                                                                                ccTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGcc                                                          >  6:261638/1‑149 (MQ=255)
                                                                                  tCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCtt                                                        >  1:59237/1‑149 (MQ=255)
                                                                                  tCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCtt                                                        >  7:169095/1‑149 (MQ=255)
                                                                                    ggTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTg                                                       <  7:301742/148‑1 (MQ=255)
                                                                                           gTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTc                                               <  7:41679/149‑1 (MQ=255)
                                                                                                            aGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTga                                                      <  2:20847/125‑1 (MQ=255)
                                                                                                              gggCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACg                            >  3:82414/1‑149 (MQ=255)
                                                                                                                   aaCGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTg                       <  8:169095/149‑1 (MQ=255)
                                                                                                                    aCGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCATGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGAc                                                   <  1:61550/121‑1 (MQ=255)
                                                                                                                    aCGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGAc                                                   >  2:61550/1‑120 (MQ=255)
                                                                                                                           gCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCa               >  3:293233/1‑149 (MQ=255)
                                                                                                                              tCCCTGGCGGGCCTGGGAGGCTGCCCGTTCGCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTc                                               >  8:265671/1‑114 (MQ=37)
                                                                                                                              tCCCTGGCGGGCCTGGGAGGCTGCCCGTTCGCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTc                                               <  7:265671/114‑1 (MQ=37)
                                                                                                                              tCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCATTg            >  1:123432/1‑149 (MQ=255)
                                                                                                                              tCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCATTg            >  8:229228/1‑149 (MQ=255)
                                                                                                                               cccAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACgg                                                 >  7:157852/1‑111 (MQ=255)
                                                                                                                               cccAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACgg                                                 <  8:157852/111‑1 (MQ=255)
                                                                                                                                 cAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCATTGCCa         <  5:261638/149‑1 (MQ=255)
                                                                                                                                      ccGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGaa                     <  6:38801/132‑1 (MQ=255)
                                                                                                                                      ccGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGaa                     >  5:38801/1‑132 (MQ=255)
                                                                                                                                      ccGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCAtt             <  7:130203/140‑1 (MQ=255)
                                                                                                                                      ccGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCAtt             >  8:130203/1‑140 (MQ=255)
                                                                                                                                        gCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCATTGCCACCTGGcc  <  2:123432/149‑1 (MQ=255)
                                                                                                                                          cAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATgg                                                                      <  6:172386/79‑1 (MQ=255)
                                                                                                                                          cAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATgg                                                                      >  5:172386/1‑79 (MQ=255)
                                                                                                                                           |                                                                                                                                                  
AGTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCA‑TGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCATTGCCACCTGGCC  >  NC_002947/3844795‑3845078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑