Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,844,941 A→T 9.8% D231E (GAT→GAA) ‡ PP_3394 ← 3‑hydroxy‑3‑methylglutaryl‑CoA lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,844,9410AT 9.8% 164.4 / 6.2 59D231E (GAT→GAA) ‡PP_33943‑hydroxy‑3‑methylglutaryl‑CoA lyase
Reads supporting (aligned to +/- strand):  ref base A (27/26);  new base T (3/3);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCATTGCCACCTGGCCCGGATTGG  >  NC_002947/3844796‑3845086
                                                                                                                                                 |                                                                                                                                                  
gTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATc                                                                                                                                                  >  5:363950/1‑148 (MQ=255)
          ggTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCaa                                                                                                                                       <  5:185038/149‑1 (MQ=255)
           gTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAAt                                                                                                                                      <  4:103264/149‑1 (MQ=255)
            tttCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGTAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATg                                                                                                                                     <  1:155764/149‑1 (MQ=255)
            tttCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATg                                                                                                                                     >  8:301742/1‑149 (MQ=255)
                 aTTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCctg                                                                                                                                >  3:24259/1‑149 (MQ=255)
                   tAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACTGGATGCCTCCCTGGCGGGCCTGGGAGGCTGCCCGTTcgcc                                                                                                                                         >  5:187035/1‑138 (MQ=25)
                   tAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACTGGATGCCTCCCTGGCGGGCCTGGGAGGCTGCCCGTTcgcc                                                                                                                                         <  6:181666/138‑1 (MQ=25)
                   tAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACTGGATGCCTCCCTGGCGGGCCTGGGAGGCTGCCCGTTcgcc                                                                                                                                         >  5:181666/1‑138 (MQ=25)
                   tAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACTGGATGCCTCCCTGGCGGGCCTGGGAGGCTGCCCGTTcgcc                                                                                                                                         <  6:187035/138‑1 (MQ=25)
                    aGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCctgct                                                                                                                              <  5:233945/148‑1 (MQ=255)
                     gcgcAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCtgctgc                                                                                                                            >  3:347303/1‑149 (MQ=255)
                        cAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCAcc                                                                                                                         <  4:347303/149‑1 (MQ=255)
                           gCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTccc                                                                                                                                          <  1:57102/129‑1 (MQ=255)
                           gCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTccc                                                                                                                                          >  2:57102/1‑129 (MQ=255)
                             aaGTCGATGCCGGTGTCGTAGCCGAGGCTTGGCAGCATGAACACCAGGTCCTGGGGCACGGTATTACCAGACGCGCCAGGGGCTAACGGGCAGCCTCCGAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGccac                                                                                                                    <  3:69078/149‑1 (MQ=255)
                             aaGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGccac                                                                                                                    >  8:41679/1‑149 (MQ=255)
                                        ggTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCa                                                                                                                                                >  2:271566/1‑110 (MQ=255)
                                        ggTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCa                                                                                                                                                <  1:271566/110‑1 (MQ=255)
                                          tgtCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGcc                                                                                                       <  6:344460/149‑1 (MQ=255)
                                            tCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCaa                                                                                                     <  6:363950/149‑1 (MQ=255)
                                                  cccATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAaggcca‑g                                                                                              >  1:110148/1‑149 (MQ=255)
                                                   ccATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAaggcca‑gg                                                                                             >  2:167848/1‑149 (MQ=255)
                                                       gCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGcccc                                                                                         >  2:361874/1‑149 (MQ=255)
                                                         tttGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCgg                                                                                       >  4:320579/1‑149 (MQ=255)
                                                         tttGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACCCGCCAGGGGCGAACGGGCAGCCTCCCAGCCCCGCCAGGGAGGCATCCAGTACCCCAATCCCCTGCTGCACCGCCACCAGGCTGTTGCCCAAGGCCA‑GGCCCCgg                                                                                       >  4:282463/1‑149 (MQ=255)
                                                           tGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCgg                                                                                       <  3:320579/147‑1 (MQ=255)
                                                               gcaTGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCa                                                                                 <  1:167848/149‑1 (MQ=255)
                                                                  tGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCg                                                                                        <  6:127780/139‑1 (MQ=255)
                                                                  tGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCg                                                                                        >  5:127780/1‑139 (MQ=255)
                                                                    aaCACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCgg                                                                                       >  3:249873/1‑138 (MQ=255)
                                                                    aaCACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCgg                                                                                       <  4:249873/138‑1 (MQ=255)
                                                                              ccTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGcc                                                                  >  6:261638/1‑149 (MQ=255)
                                                                                tCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCtt                                                                >  7:169095/1‑149 (MQ=255)
                                                                                tCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCtt                                                                >  1:59237/1‑149 (MQ=255)
                                                                                  ggTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTg                                                               <  7:301742/148‑1 (MQ=255)
                                                                                         gTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTc                                                       <  7:41679/149‑1 (MQ=255)
                                                                                                          aGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTga                                                              <  2:20847/125‑1 (MQ=255)
                                                                                                            gggCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACg                                    >  3:82414/1‑149 (MQ=255)
                                                                                                                 aaCGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTg                               <  8:169095/149‑1 (MQ=255)
                                                                                                                  aCGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCATGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGAc                                                           <  1:61550/121‑1 (MQ=255)
                                                                                                                  aCGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGAc                                                           >  2:61550/1‑120 (MQ=255)
                                                                                                                         gCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCa                       >  3:293233/1‑149 (MQ=255)
                                                                                                                            tCCCTGGCGGGCCTGGGAGGCTGCCCGTTCGCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTc                                                       >  8:265671/1‑114 (MQ=37)
                                                                                                                            tCCCTGGCGGGCCTGGGAGGCTGCCCGTTCGCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTc                                                       <  7:265671/114‑1 (MQ=37)
                                                                                                                            tCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCATTg                    >  1:123432/1‑149 (MQ=255)
                                                                                                                            tCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCATTg                    >  8:229228/1‑149 (MQ=255)
                                                                                                                             cccAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACgg                                                         <  8:157852/111‑1 (MQ=255)
                                                                                                                             cccAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACgg                                                         >  7:157852/1‑111 (MQ=255)
                                                                                                                               cAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCATTGCCa                 <  5:261638/149‑1 (MQ=255)
                                                                                                                                    ccGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGaa                             <  6:38801/132‑1 (MQ=255)
                                                                                                                                    ccGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGaa                             >  5:38801/1‑132 (MQ=255)
                                                                                                                                    ccGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCAtt                     <  7:130203/140‑1 (MQ=255)
                                                                                                                                    ccGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCAtt                     >  8:130203/1‑140 (MQ=255)
                                                                                                                                      gCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCATTGCCACCTGGcc          <  2:123432/149‑1 (MQ=255)
                                                                                                                                        cAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATgg                                                                              >  5:172386/1‑79 (MQ=255)
                                                                                                                                        cAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATgg                                                                              <  6:172386/79‑1 (MQ=255)
                                                                                                                                          gggAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCATTGCCACCTGGCCCGGa      >  1:116326/1‑149 (MQ=255)
                                                                                                                                              ggCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCATTGCCACCTGGCCCGGATTgg  <  2:116326/149‑1 (MQ=255)
                                                                                                                                                 aTCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCAtt                     >  6:18514/1‑127 (MQ=255)
                                                                                                                                                 aTCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCAtt                     <  5:18514/127‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCA‑GGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCATTGCCACCTGGCCCGGATTGG  >  NC_002947/3844796‑3845086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑