Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,881,829	A→T	12.7%	Q163L (CAG→CTG)	oprN →	multidrug RND transporter outer membrane protein OprN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,881,829	0	A	T	12.7%	183.8 / 16.4	71	Q163L (CAG→CTG)	oprN	multidrug RND transporter outer membrane protein OprN
Reads supporting (aligned to +/- strand):  ref base A (32/30);  new base T (4/5);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 7.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

CCGGCCAGACCACCGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTACCGAAACCCTGCGCGATGCGGGCGTGGG  >  NC_002947/3881685‑3881976                                                                                                                                                    |                                                                                                                                                    ccGGCCAGACCACCGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACTGTTg                                                                                                                                                 <  3:10408/149‑1 (MQ=255)  cGGCCAGACCACCGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGc                                                                                                                                                <  3:82356/149‑1 (MQ=255)   ggCCAGACCACCGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCa                                                                                                                                               <  3:258753/149‑1 (MQ=255)      cAGACCACCGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACTGTTGCAGGt                                                                                                                                            <  3:262009/149‑1 (MQ=255)         accaccGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAg                                                                                                                                         >  3:346301/1‑149 (MQ=255)           caccGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACTGTTGCAGGt                                                                                                                                            <  1:358122/144‑1 (MQ=255)           caccGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACTGTTGCAGGt                                                                                                                                            >  2:358122/1‑144 (MQ=255)           caccGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAg                                                                                                                                              >  4:82035/1‑142 (MQ=255)           caccGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAg                                                                                                                                              <  3:82035/142‑1 (MQ=255)           caccGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGcc                                                                                                                                       >  8:363060/1‑149 (MQ=255)            accGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCt                                                                                                                                      >  4:41829/1‑149 (MQ=255)             ccGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCt                                                                                                                                      >  6:44345/1‑148 (MQ=255)              cGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACTGTTGCAGGTCGGcggcg                                                                                                                                    <  7:201834/149‑5 (MQ=255)               gAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCACCGCCGACCTGCAACAGTTGCAGGTCAGCCTGAt                                                                                                                                   <  8:348487/149‑1 (MQ=255)                        aaTAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAAc                                                                                                                           <  3:336125/148‑1 (MQ=255)                        aaTAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAAc                                                                                                                           <  2:77255/148‑1 (MQ=255)                        aaTAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACt                                                                                                                          >  3:137755/1‑149 (MQ=255)                             cgagcgCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTc                                                                                                                      >  3:59958/1‑148 (MQ=255)                               atcgcTAAGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCATCGCCAGATCGAAGCCATCGCACCAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTc                                                                                                                      <  1:308021/147‑1 (MQ=255)                                    tACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTa                                                                                                              >  2:261824/1‑149 (MQ=255)                                      cGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACg                                                                                                            >  5:61825/1‑149 (MQ=255)                                       gACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACgg                                                                                                           <  1:261824/149‑1 (MQ=255)                                         ccTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGAt                                                                                                                   >  8:300391/1‑139 (MQ=255)                                         ccTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGAt                                                                                                                   <  7:300391/139‑1 (MQ=255)                                              gCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTg                                                                                                    >  1:240757/1‑149 (MQ=255)                                               ccTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGAAACTgc                                                                                                   >  6:178584/1‑149 (MQ=255)                                                   gACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGcgcg                                                                                                <  4:346301/148‑1 (MQ=255)                                                         gCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGcc                                                                                                                                       >  1:127099/1‑103 (MQ=255)                                                         gCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGcc                                                                                                                                       <  2:127099/103‑1 (MQ=255)                                                         gCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGccc                                                                                          <  5:44345/148‑1 (MQ=255)                                                           cTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGt                                                                                       >  3:172927/1‑149 (MQ=255)                                                              ggAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGGGCACCGCGCAGTTgc                                                                                    <  4:137755/149‑1 (MQ=255)                                                              ggAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGtt                                                                                      <  4:172927/147‑1 (MQ=255)                                                                    tGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGaa                                                                               >  7:265075/1‑148 (MQ=255)                                                                          gTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCa                                                                                                                                          >  4:119728/1‑83 (MQ=255)                                                                          gTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCa                                                                                                                                          <  3:119728/83‑1 (MQ=255)                                                                          gTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCg                                                                        >  4:48076/1‑149 (MQ=255)                                                                           ttCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGTAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGc                                                                       >  1:264620/1‑149 (MQ=255)                                                                                      ccAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAAcc                                                            >  8:194843/1‑149 (MQ=255)                                                                                         gcgcCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTg                                                          >  1:329181/1‑148 (MQ=255)                                                                                         gcgcCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTg                                                          >  5:357164/1‑148 (MQ=255)                                                                                          cgcCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGaa                                                        <  4:59958/149‑1 (MQ=255)                                                                                            ccAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGGGCACCGCGCAGTTgccc                                                                                  <  2:309477/121‑3 (MQ=255)                                                                                            ccAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGGGCACCGCGCAGTTgccc                                                                                  >  1:309477/1‑119 (MQ=255)                                                                                             cAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGAc                                                     >  3:298224/1‑149 (MQ=255)                                                                                                 tCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCcag                                                 <  3:41829/149‑1 (MQ=255)                                                                                                   gAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCcagca                                               <  5:204137/149‑1 (MQ=255)                                                                                                    aaGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCcagca                                               <  7:363060/148‑1 (MQ=255)                                                                                                      gCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCg                                                                                                                              >  1:181801/1‑67 (MQ=255)                                                                                                      gCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCg                                                                                                                              <  2:181801/67‑1 (MQ=255)                                                                                                         aGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAAt                                          >  8:69998/1‑148 (MQ=255)                                                                                                         aGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAAt                                          >  1:235437/1‑148 (MQ=255)                                                                                                                    ccAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCa                                 >  5:41680/1‑149 (MQ=255)                                                                                                                     cAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCCCCAt                                >  7:185623/1‑149 (MQ=255)                                                                                                                     cAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCAt                                >  4:354796/1‑149 (MQ=255)                                                                                                                     cAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCAt                                <  3:233615/149‑1 (MQ=255)                                                                                                                                      ggctgACCTGCAACTGTTGCAGGTCGGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTACCGAAACCCTgcgc                >  7:233901/5‑148 (MQ=255)                                                                                                                                       gccgACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTACCGAAACCCTGCGCGa              <  2:240757/149‑1 (MQ=255)                                                                                                                                          gACCTGCAACTGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTACCGAAACCCTGCGCGATGc           >  6:272536/1‑149 (MQ=255)                                                                                                                                          gACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTACCGAAACCCTGCGCGATGc           <  2:235437/149‑1 (MQ=255)                                                                                                                                           aCCTGCAACTGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGaa                                                        >  4:347955/1‑103 (MQ=255)                                                                                                                                           aCCTGCAACTGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGaa                                                        <  3:347955/103‑1 (MQ=255)                                                                                                                                               gCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCAt                                <  4:160892/123‑1 (MQ=255)                                                                                                                                               gCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCAt                                >  3:160892/1‑123 (MQ=255)                                                                                                                                                cAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAg                                                                            >  5:323378/1‑78 (MQ=255)                                                                                                                                                cAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAg                                                                            <  6:323378/78‑1 (MQ=255)                                                                                                                                                cAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTgg                                                                  >  3:259333/1‑88 (MQ=255)                                                                                                                                                cAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTgg                                                                  <  4:259333/88‑1 (MQ=255)                                                                                                                                                cAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTACCGAAACCCTGCGCGATGCGGGCGt     <  8:185623/149‑1 (MQ=255)                                                                                                                                                 aaCAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTACCGAAACCCTGCGCGATGCGGGCGt     <  7:194843/148‑1 (MQ=255)                                                                                                                                                   cAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAAGCTGAAGACCCAGCAGGAATCACGCACCATTACCGAAACCCTGCGCGATGCGGGCGTggg  <  6:357164/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CCGGCCAGACCACCGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCG‑A‑‑AGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTACCGAAACCCTGCGCGATGCGGGCGTGGG  >  NC_002947/3881685‑3881976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑