Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,913,114 A→G 10.0% L322P (CTG→CCG)  PP_3453 ← sensor protein RstB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,913,1140AG10.0% 108.4 / 2.4 40L322P (CTG→CCG) PP_3453sensor protein RstB
Reads supporting (aligned to +/- strand):  ref base A (21/15);  new base G (2/2);  total (23/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

AGGGACGCCAATCCCGTCATCCTCCACCGTCAGCAGGCAGGCGCCATCGGCTTCCAGGCGCAAGCGCAGCAGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAGCTCCAGCAGCGATACCTCCACCCGCTCCCGCGCCTGGTCGGCGTTGTACAA  >  NC_002947/3912969‑3913262
                                                                                                                                                 |                                                                                                                                                    
aGGGACGCCAATCCCGTCATCCTCCACCGTCAGCAGGCAGGCGCCATCGGCTTCCAGGCGCAAGCGCAGCAGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTa                                                                                                                                                   >  3:351896/1‑149 (MQ=255)
      gCCAATCCCGTCATCCTCCACCGTCAGCAGGCAGGCGCCATCGGCTTCCAGGCGCAAGCGCAGCAGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAgc                                                                                                                                             >  4:343892/1‑149 (MQ=255)
        cAATCCCGTCATACTCCACCGTCAGCAGGCAGGAGCCATCGGCTTCCAGGCTCAAGCGCAGCAGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAgcgc                                                                                                                                           <  8:262021/149‑1 (MQ=255)
           tCCCGTCATCCTCCACCGTCAGCAGGCAGGCGCCATCGGCTTCCAGGCGCAAGCGCAGCAGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCgg                                                                                                                                         >  8:155620/1‑148 (MQ=255)
                    cctccACCGTCAGCAGGCAGGCGCCATCGGCTTCCAGGCGCAAGCGCAGCAGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCAccc                                                                                                                               >  2:58484/1‑149 (MQ=255)
                        cACCGTCAGCAGGCAGGCGCCATCGGCTTCCAGGCGCAAGCGCAGCAGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCgg                                                                                                                           >  3:314238/1‑149 (MQ=255)
                         aCCGTCAGCAGGCAGGCGCCATCGGCTTCCAGGCGCAAGCGCAGCAGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACACTCGGc                                                                                                                          >  4:346191/1‑149 (MQ=255)
                          ccGTCAGCAGGCAGGCGCCATCGGCTTCCAGGCGCAAGCGCAGCAGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCGGTACAAAGCGCGGCAACTCACCCTCGGcc                                                                                                                         >  7:203487/1‑149 (MQ=255)
                          ccGTCAGCAGGCAGGCGCCATCGGCTTCCAGGCGCAAGCGCAGCAGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCGGTACAAAGCGCGGCAACTCACCCTCGGcc                                                                                                                         >  7:197929/1‑149 (MQ=255)
                                gcaggcagGCGCCATCGGCTTCCAGGCGCAAGCGCAGCAGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCCCCCTCGGCCCGCAcc                                                                                                                   >  3:294596/1‑149 (MQ=255)
                                                  cTTCCAGGCGCAAGCGCAGCAGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCgg                                                                                                 <  4:351896/149‑1 (MQ=255)
                                                         gcgcAAGCGCAGCAGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGa                                                                                          <  3:343892/149‑1 (MQ=255)
                                                           gcAAGCGCAGCAGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCa                                                                                                                                                      <  4:4127/87‑1 (MQ=255)
                                                           gcAAGCGCAGCAGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCa                                                                                                                                                      >  3:4127/1‑87 (MQ=255)
                                                             aaGCGCAGCAGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCgg                                                                                                                                         >  3:172383/1‑98 (MQ=255)
                                                             aaGCGCAGCAGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCgg                                                                                                                                         <  4:172383/98‑1 (MQ=255)
                                                               gcgcAGCGGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCt                                                                                    >  3:6572/1‑149 (MQ=255)
                                                                   agcagcagGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGc                                                                                <  4:314238/149‑1 (MQ=255)
                                                                     cagcagGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCa                                                                               >  8:233930/1‑148 (MQ=255)
                                                                      agcagGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCa                                                                               >  5:84276/1‑147 (MQ=255)
                                                                        cagGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGc                                                                           >  3:66284/1‑149 (MQ=255)
                                                                        cagGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGc                                                                           >  6:243248/1‑149 (MQ=255)
                                                                            cTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGccccc                                                                       <  3:346191/149‑1 (MQ=255)
                                                                                    gCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGcaca                                                                <  1:58484/148‑1 (MQ=255)
                                                                                           cgcATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGAc                                                        >  8:12517/1‑149 (MQ=255)
                                                                                            gcATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGAcc                                                       >  3:2454/1‑149 (MQ=255)
                                                                                              aTGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAg                                                     <  7:233930/149‑1 (MQ=255)
                                                                                                   aTTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAGCTCca                                                <  7:155620/149‑1 (MQ=255)
                                                                                                       cgcACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCGGTACAAAGCGCTGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAGCTCcagca                                             <  8:197929/148‑1 (MQ=255)
                                                                                                       cgcACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCGGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCAGCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAGCTCcagca                                             <  8:203487/148‑1 (MQ=255)
                                                                                                          aCCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGc                                                                           >  2:84880/1‑115 (MQ=255)
                                                                                                                  cTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAGCTCCAGCAGCGATACCTCCa                                 >  3:217329/1‑149 (MQ=255)
                                                                                                                          cgcgcgcCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAGCTCCAGCAGCGATACCTCCACCCGCTcc                         >  8:5328/1‑149 (MQ=255)
                                                                                                                             gcgcCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAGCTCCAGCAGCGATACCTCCACCCGCTCCcgc                      >  3:118518/1‑149 (MQ=255)
                                                                                                                                  gTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAGCTCCAGCAGCGATACCTCCACCCGCTCCCGCGCCTg                 <  6:84276/149‑1 (MQ=255)
                                                                                                                                  gTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAGCTCCAGCAGCGATACCTCCACCCGCTCCCGCGCCTg                 <  4:118518/149‑1 (MQ=255)
                                                                                                                                   tCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAGCTCCAGCAGCGATACCTCCACCCGCTCCCGCGCCTgg                >  6:193458/1‑149 (MQ=255)
                                                                                                                                      gTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATGTAGTTCCTCGGGAAATCCCCCAAGCACACTGTCGACCAGCTCCAGCAGCGATACCTCCACCCGCTCCCGCGCCTGGTCg             <  5:272579/149‑1 (MQ=255)
                                                                                                                                           gggTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAGCTCCAGCAGCGATACCTCCACCCGCTCCCGCGCCTGGTCGGCGtt        <  5:243248/149‑1 (MQ=255)
                                                                                                                                            ggTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAGCTCCAGCAGCGATACCTCCACCCGCTCCCGCTCCTGGTCGGCGTTg       <  5:118849/149‑1 (MQ=255)
                                                                                                                                             gTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAGCTCCAGCAGCGATACCTCCACCCGCTCCCGCGCCTGGTCGGCGTTGt      <  4:66284/149‑1 (MQ=255)
                                                                                                                                               ccAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAGCTCCAGCAGCGATACCTCCACCCGCTCCCGCGCCTGGTCGGCGTTGTa     >  4:122973/1‑148 (MQ=255)
                                                                                                                                                 aGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAGCTCCAGCAGCTATACCTCCACCCGCTCCCGCGCCTGGTCGGCGTTGTACa   >  6:274184/1‑148 (MQ=255)
                                                                                                                                                 aGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAGCTCCAGCAGCGATACCTCCACCCGCTCCCGCGCCTGGTCGGCGTTGTACaa  >  6:12139/1‑149 (MQ=255)
                                                                                                                                                 aGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACATCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAGCTACAGAACCGATACCTCCACCCGCTCACTCGACTGGTCGGCGTTTTACa   >  6:274187/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
AGGGACGCCAATCCCGTCATCCTCCACCGTCAGCAGGCAGGCGCCATCGGCTTCCAGGCGCAAGCGCAGCAGCAGGCTTTCATCGCAATAGCGCATGGCATTGCGCACCAGGTTCTGCACCGCGCGCGCCGTCAGTCGCGGGTCCAGTACAAAGCGCGGCAACTCACCCTCGGCCCGCACCTCCCACTGGATACCCCGGCCATCGAGTTCCTCGGCAAAGCCCCCAAGCACACTGTCGACCAGCTCCAGCAGCGATACCTCCACCCGCTCCCGCGCCTGGTCGGCGTTGTACAA  >  NC_002947/3912969‑3913262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑