Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,919,303 C→T 7.5% Q1029* (CAG→TAG)  mexB → multidrug resistance protein MexB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,919,3030CT 7.5% 151.6 / 3.0 53Q1029* (CAG→TAG) mexBmultidrug resistance protein MexB
Reads supporting (aligned to +/- strand):  ref base C (25/24);  new base T (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

CCTGGCCATCGCCAGCGGCGCAAGCTCTGCCAGCCAGCAGGCGATT‑GGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTC  >  NC_002947/3919161‑3919445
                                                                                                                                               |                                                                                                                                              
ccTGGCCATCGCCAGCGGCGCAAGCTCTGCCAGCCAGCAGGCGATTGGGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGgcg                                                                                                                                           >  1:89252/1‑149 (MQ=255)
 cTGGCCATCGCCAGCGGCGCAAGCTCTGCCAGCCAGCAGGCGATT‑GGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCTAGGCGCa                                                                                                                                         >  7:101316/1‑149 (MQ=255)
 cTGGCCATCGCCAGCGGCGCAAGCTCTGCCAGCCAGCAGGCGATT‑GGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCTAGGCGCa                                                                                                                                         >  7:106679/1‑149 (MQ=255)
    gCCATCGCCAGTGGCGCAAGCTCTGCCAGCCAGCAGGCGATT‑GGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATg                                                                                                                                       <  5:97621/148‑1 (MQ=255)
    gCCATCGCCAGCGGCGCAAGCTCTGCCAGCCAGCAGGCGATT‑GGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATg                                                                                                                                       <  1:378613/148‑1 (MQ=255)
    gCCATCGCCAGCGGCGCAAGCTCTGCCAGCCAGCAGGCGATT‑GGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATg                                                                                                                                       <  2:89252/148‑1 (MQ=255)
          gCCAGCGGCGCAAGCTCTGCCAGCCAGCAGGCGATT‑GGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGa                                                                                                                                >  8:214085/1‑149 (MQ=255)
             aGCGGCGCAAGCTCTGCCAGCCAGCAGGCGATT‑GGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACgg                                                                                                                             >  1:87640/1‑149 (MQ=255)
             aGCGGCGCAAGCTCTGCCAGCCAGCAGGCGATT‑GGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACgg                                                                                                                             <  3:373118/149‑1 (MQ=255)
              gcggcgCAAGCTCTGCCAGCCAGCAGGCGATT‑GGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACggg                                                                                                                            <  7:223313/149‑1 (MQ=255)
                  cgcAAGCTCTGCCAGCCAGCAGGCGATT‑GGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAAc                                                                                                                        <  8:159245/149‑1 (MQ=255)
                      aGCTCTGCCAGCCAGCAGGCGATT‑GGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCTAGGCGCATGACAGCGACGGGCAACCCGt                                                                                                                    <  8:106679/149‑1 (MQ=255)
                      aGCTCTGCCAGCCAGCAGGCGATT‑GGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCTAGGCGCATGACAGCGACGGGCAACCCGt                                                                                                                    <  8:101316/149‑1 (MQ=255)
                            gccagccaGCAGGCGATT‑GGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCcg                                                                                                              >  8:89343/1‑149 (MQ=255)
                             ccaggcagCAGGCGATT‑GGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCcgc                                                                                                             >  2:348715/1‑149 (MQ=255)
                              cagccagcAGGCGATT‑GGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCcgcg                                                                                                            <  7:214085/149‑1 (MQ=255)
                              cagccagcAGGCGATT‑GGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCcgc                                                                                                             >  5:152710/1‑148 (MQ=255)
                                                    cGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGcccc                                                                                       <  7:89343/149‑1 (MQ=255)
                                                      gggTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTg                                                                                                                   >  2:214082/1‑119 (MQ=255)
                                                      gggTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTg                                                                                                                   <  1:214082/119‑1 (MQ=255)
                                                        gTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCt                                                                                   <  3:237897/149‑1 (MQ=255)
                                                         tGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACa                                                                                                                                    <  7:37722/99‑1 (MQ=255)
                                                         tGATCGGCGGAATGCACAGCGCCACCCTGGCGCAGGTGTTCGTGCCGGTGTTGTTCGTGGTGGTGATGCGCCTGTACGGGCGAATCCAGCAGCATGACa                                                                                                                                    >  8:37722/1‑99 (MQ=255)
                                                              ggcggcATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATg                                                                             >  4:304793/1‑149 (MQ=255)
                                                              ggcggcATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATg                                                                             <  5:246688/149‑1 (MQ=255)
                                                               gcggcATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCcg                                                                                                              <  8:245845/115‑1 (MQ=255)
                                                               gcggcATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCcg                                                                                                              >  7:245845/1‑115 (MQ=255)
                                                                 ggcATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGa                                                                          <  1:348715/149‑1 (MQ=255)
                                                                   cATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAgg                                                                        <  6:152710/149‑1 (MQ=255)
                                                                    aTGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCACGCGAAAGCTGACTGGACCAGCCCCGCCTGAAATGGGAggg                                                                       >  7:163125/1‑149 (MQ=255)
                                                                       cTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGaaa                                                                                                         <  1:373082/112‑1 (MQ=255)
                                                                       cTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGaaa                                                                                                         >  2:373082/1‑112 (MQ=255)
                                                                        tCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACgg                                                                                                                             <  6:255535/91‑1 (MQ=255)
                                                                        tCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACgg                                                                                                                             >  5:255535/1‑91 (MQ=255)
                                                                          aGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGa                                                                                               >  3:90942/1‑119 (MQ=255)
                                                                          aGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCg                                                                 >  5:73563/1‑149 (MQ=255)
                                                                          aGCGCCACCCTGGCGGTGGTGTTCGAGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCg                                                                 >  5:95800/1‑149 (MQ=255)
                                                                              ccACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCaa                                                             <  2:87640/149‑1 (MQ=255)
                                                                               cACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAAt                                                            >  2:41224/1‑149 (MQ=255)
                                                                                                  gTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCtttt                                         <  3:291747/149‑1 (MQ=255)
                                                                                                      cGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCAt                                     <  3:304793/149‑1 (MQ=255)
                                                                                                       ggTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGCCGGGCAACCCGTGCCCGGCGAAAGATGACTGGACAAGCCCCGCCGGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTGTTTCATg                                    >  6:125735/1‑149 (MQ=255)
                                                                                                            tcttcGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGa                                                                          <  7:111218/106‑1 (MQ=255)
                                                                                                            tcttcGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGa                                                                          >  8:111218/1‑106 (MQ=255)
                                                                                                                    gtggtgATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTcc                       >  3:322776/1‑149 (MQ=255)
                                                                                                                       gtgATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTccccc                    >  6:76872/1‑149 (MQ=255)
                                                                                                                         gATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTccccccc                  >  2:279149/1‑149 (MQ=255)
                                                                                                                            gcgcCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGc               >  8:193512/1‑149 (MQ=255)
                                                                                                                              gcCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGGCAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCAt                                     <  7:87482/125‑1 (MQ=255)
                                                                                                                              gcCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGGCAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCAt                                     >  8:87482/1‑125 (MQ=255)
                                                                                                                                    ccGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCt       >  8:255674/1‑149 (MQ=255)
                                                                                                                                      gggCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCa     <  6:95800/149‑1 (MQ=255)
                                                                                                                                         cGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATtc  <  6:73563/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                              
CCTGGCCATCGCCAGCGGCGCAAGCTCTGCCAGCCAGCAGGCGATT‑GGCACCGGGGTGATCGGCGGCATGCTCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTC  >  NC_002947/3919161‑3919445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑