Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,953,944 G→A 7.4% V193V (GTC→GTT)  PP_5606 ← YVTN beta‑propeller repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,953,9440GA 7.4% 157.1 / 3.1 54V193V (GTC→GTT) PP_5606YVTN beta‑propeller repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (28/22);  new base A (2/2);  total (30/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

ATCACTGTTACCCCACCTTTGGCCGGGTCGCTGCTGTTGTTGCCAACCCACAGCCGATGCTGGTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGTCACGCCCCCCACCGACACCGTCGGGTCGATCACTG  >  NC_002947/3953798‑3954083
                                                                                                                                                   |                                                                                                                                           
aTCACTGTTACCCCACCTTTGGCCGGGTCGCTGCTGTTGTTGCCAACCCACAGCCGATGCTGGTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGAc                                                                                                                                           <  8:86385/149‑1 (MQ=255)
aTCACTGTTACCCCACCTTTGGCCGGGTCGCTGCTGTTGTTGCCAACCCACAGCCGATGCTGGTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGAc                                                                                                                                           >  3:288798/1‑149 (MQ=255)
           cccACCTTTGGCCGGGTCGCTGCTGTTGTTGCCAACCCACAGCCGATGCTGGTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCt                                                                                                                                >  4:116840/1‑149 (MQ=255)
                    ggCCGGGTCGCTGCTGTTGTTGCCAACCCACAGCCGATGCTGGTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATc                                                                                                                        <  4:327587/148‑1 (MQ=255)
                     gCCGGGTCGCTGCTGTTGTTGCCAACCCACAGCCGATGCTGGTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGa                                                                                                                      >  2:379219/1‑149 (MQ=255)
                               tgctgTTGTTGCCAACCCACAGCCGATGCTGGTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAAcc                                                                                                            >  8:317182/1‑149 (MQ=255)
                                gctgTTGTTGCCAACCCACAGCCGATGCTGGTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCt                                                                                                           >  4:184941/1‑149 (MQ=255)
                                          ccAACCCACAGCCGATGCTGGTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTgtcgtc                                                                                                 >  8:350457/1‑149 (MQ=255)
                                            aaCCCACAGCCGATGCTGGTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGc                                                                                               >  1:38847/1‑149 (MQ=255)
                                                       gATGCTGGTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGgccgcc                                                                                    <  4:288798/149‑1 (MQ=255)
                                                         tGCTGGTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCa                                                                                  <  3:184941/149‑1 (MQ=255)
                                                           cTGGTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGt                                                                                <  2:38847/149‑1 (MQ=255)
                                                            tGGTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTc                                                                               >  6:75042/1‑149 (MQ=255)
                                                            tGGTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCAGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTc                                                                               >  4:322588/1‑149 (MQ=255)
                                                             ggTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTc                                                                               >  6:230169/1‑148 (MQ=255)
                                                             ggTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTc                                                                               >  7:117377/1‑148 (MQ=255)
                                                              gTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTAAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGt                                                                                <  6:270758/146‑1 (MQ=255)
                                                              gTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTAAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGt                                                                                >  5:270758/1‑146 (MQ=255)
                                                               tCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCAt                                                                            >  7:42145/1‑149 (MQ=255)
                                                                 cGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATgg                                                                          <  6:234055/149‑1 (MQ=255)
                                                                 cGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATg                                                                           <  3:116840/148‑1 (MQ=255)
                                                                  ggCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATggg                                                                         <  7:350457/149‑1 (MQ=255)
                                                                      gCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCtggtgg                                                                                         >  2:324840/1‑129 (MQ=255)
                                                                      gCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGgccgc                                                                     >  7:308427/1‑149 (MQ=255)
                                                                      gCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGgccgc                                                                     >  6:184427/1‑149 (MQ=255)
                                                                        aGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGccc                                                                   <  5:75042/149‑1 (MQ=255)
                                                                           gCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTGCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTgg                                                                <  5:85638/149‑1 (MQ=255)
                                                                            ccACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTgg                                                                >  4:322258/1‑148 (MQ=255)
                                                                                gcgcAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCt                                                           <  8:42145/149‑1 (MQ=255)
                                                                                 cgcAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTc                                                          <  8:308427/149‑1 (MQ=255)
                                                                                  gcAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTc                                                          >  1:376116/1‑148 (MQ=255)
                                                                                     cgaccTGGACGCCGGCGACCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATg                                                      >  8:106812/5‑149 (MQ=255)
                                                                                      cGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATgc                                                     <  3:311477/149‑1 (MQ=255)
                                                                                       accTGGACGCCGGCGACCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGTGCCGCCCTGGCAGCTCGATgcg                                                    >  3:130783/3‑149 (MQ=255)
                                                                                        gCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATgcgc                                                   <  7:317182/149‑1 (MQ=255)
                                                                                                         aGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGTCAc                                  >  3:110919/1‑149 (MQ=255)
                                                                                                         aGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGTCAc                                  >  4:137282/1‑149 (MQ=255)
                                                                                                                ccgagcgGGGCAAGGTCTCGGTGATCGAT‑ACCGAAACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGTCACGccccc                            >  4:257293/5‑148 (MQ=38)
                                                                                                                ccgagcgGGGCAAGGTCTCGGTGATCGAT‑ACCGAAACCTTCACCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGTCACGccccc                            >  6:164534/5‑148 (MQ=37)
                                                                                                                          gAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTg                                       <  3:298802/127‑1 (MQ=255)
                                                                                                                          gAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTg                                       >  4:298802/1‑127 (MQ=255)
                                                                                                                              gTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGTCACGCCCTCCACCGACACCGTCgg             >  1:46799/1‑149 (MQ=255)
                                                                                                                                 tCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGTCACGCCCCCCACCGACACCGTCGGGt           <  5:184427/148‑1 (MQ=255)
                                                                                                                                 tCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGTCACGCCCCCCACCGACACCGTCGGGt           <  4:110919/148‑1 (MQ=255)
                                                                                                                                   ggtgaTCGAT‑ACCGAAACCTTCACCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGTCACGCCCCCCACCGACACCGTCGGGt           <  5:164534/142‑1 (MQ=255)
                                                                                                                                    gtgaTCGAT‑ACCGAAACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGTCACGCCCCCCACCGACACCGTCGGGTCGAt       <  3:257293/146‑1 (MQ=255)
                                                                                                                                       aTCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGCCACGcccc                             >  7:316521/1‑125 (MQ=255)
                                                                                                                                       aTCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGCCACGcccc                             <  8:316521/125‑1 (MQ=255)
                                                                                                                                         cGATCACCGAGACCTTGCCCCGCTCGGGTAACGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGTCACGCCCCCCACCGACACCGTCGGGTCGATCACt   >  1:30136/1‑149 (MQ=255)
                                                                                                                                          gATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGTCACGCCCCCCACCGACACCGTCGGGTCGATCACTg  <  3:137282/149‑1 (MQ=255)
                                                                                                                                            tCACCGTGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGTCACGCCCCCCACCGACACCGTCGGGTCGATCACt   <  3:67657/146‑1 (MQ=255)
                                                                                                                                            tCACCGTGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGTCACGCCCCCCACCGACACCGTCGGGTCGATCACt   >  4:67657/1‑146 (MQ=255)
                                                                                                                                                cGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGTCACGcc                               >  4:125132/1‑114 (MQ=255)
                                                                                                                                                cGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGTCACGcc                               <  3:125132/114‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
ATCACTGTTACCCCACCTTTGGCCGGGTCGCTGCTGTTGTTGCCAACCCACAGCCGATGCTGGTCCGGCTGCAGGGCCACGCGCAGCGGCTGGTCGCCGGCGTCCAGGTCGGCCACGCGGGTGAAGGTTTCGGT‑ATCGATCACCGAGACCTTGCCCCGCTCGGGTATCGACACGAAAACCTGCTTGTCGTCGCTGGTGGCCGCCCAGTCCATGGGCCGCCCTGGCAGCTCGATGCGCGCCATGGTGCTGGTCACGCCCCCCACCGACACCGTCGGGTCGATCACTG  >  NC_002947/3953798‑3954083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑