| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,960,613 | T→C | 7.3% | intergenic (‑229/‑100) | mnxG ← / → PP_3491 | manganese‑oxidizing multicopper oxidase/enoly‑coenzyme A hydratase/isomerase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,960,613 | 0 | T | C | 7.3% | 155.0 / 3.1 | 55 | intergenic (‑229/‑100) | mnxG/PP_3491 | manganese‑oxidizing multicopper oxidase/enoly‑coenzyme A hydratase/isomerase family protein |
| Reads supporting (aligned to +/- strand): ref base T (25/26); new base C (2/2); total (27/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.82e-01 | |||||||||||
AACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACAGACCATGTA > NC_002947/3960465‑3960754 | aaCTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAAtt < 3:233276/149‑1 (MQ=255) cGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATtg > 1:65641/1‑149 (MQ=255) cAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGc > 1:91976/1‑148 (MQ=255) aaCCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATCGACAATTGTGCaa < 8:39243/149‑1 (MQ=255) aaCCGCAACGGATTGGGGAGGATACCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATCGACAATTGTGCa < 4:211368/148‑1 (MQ=255) cAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAg < 5:360020/145‑1 (MQ=255) cAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAg > 6:360020/1‑145 (MQ=255) gATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCg > 2:125556/1‑149 (MQ=255) ttGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCa < 8:280582/149‑1 (MQ=255) gggAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGc > 4:285797/1‑149 (MQ=255) ggaggaTCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGcc < 3:9373/149‑1 (MQ=255) tCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAgg > 2:217399/1‑129 (MQ=255) tCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAgg < 1:217399/129‑1 (MQ=255) cccAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGa > 5:48085/1‑144 (MQ=255) cccAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGa < 6:48085/144‑1 (MQ=255) cccAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTg < 8:137781/149‑1 (MQ=255) gcagGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGAt > 1:78261/1‑149 (MQ=255) gTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGAtcctc > 6:261855/1‑149 (MQ=255) ttCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTg < 6:374693/140‑1 (MQ=255) ttCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTg > 5:374693/1‑140 (MQ=255) ccGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCg > 4:222993/1‑148 (MQ=255) ccGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCg > 8:94041/1‑148 (MQ=255) ccGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCCATCCTCCAGCg > 3:26145/1‑148 (MQ=255) ggggACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGa < 5:261855/149‑1 (MQ=255) aacctgctGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACtt < 4:15898/142‑1 (MQ=255) tCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTg > 2:125254/1‑148 (MQ=255) aaGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAAc > 1:339206/1‑148 (MQ=255) aaGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACa < 2:78261/149‑1 (MQ=255) ctCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAgg < 2:91976/149‑1 (MQ=255) tCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTTCCCTTGCACAAtt > 7:344974/1‑58 (MQ=255) tCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTTCCCTTGCACAAtt < 8:344974/58‑1 (MQ=255) tCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAgg > 4:250431/1‑148 (MQ=255) tCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAgg > 6:174795/1‑148 (MQ=255) cacaAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCACCGAACTCAACCAATCACTTGGAAAATCAAAAACAAACCAGTgag > 8:322105/1‑149 (MQ=255) aaGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCt < 7:293648/90‑1 (MQ=255) aaGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCt > 8:293648/1‑90 (MQ=255) cgcTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGa < 3:222993/149‑1 (MQ=255) cgcTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGa < 1:240789/149‑1 (MQ=255) gcTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCATAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGAc < 1:125556/149‑1 (MQ=255) gCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGca < 4:224778/149‑1 (MQ=255) acacaAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCAAGCGATCATCCAGCGAACTCAAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGcaccag > 3:66529/1‑145 (MQ=255) caAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCa < 7:94041/149‑1 (MQ=255) aaGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCa > 3:187917/1‑148 (MQ=255) ggTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCa < 6:133600/88‑1 (MQ=255) ggTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCa > 5:133600/1‑88 (MQ=255) ggTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATAc > 5:100159/1‑149 (MQ=255) gTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACt > 3:359454/1‑134 (MQ=255) gTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACt < 4:359454/134‑1 (MQ=255) aaaaCGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATAcccc < 3:117611/148‑1 (MQ=255) aCGCACGGTACCCTTGCACAATTGTCGATTGTGCAATGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGgca < 7:322105/149‑1 (MQ=255) tccccTTGCACATTTGTCGATTGTGAAAGGAAAATTCGCATGCCAGGATCCTGCGATCCTCCACCGAACTCCAGACTTCACTTGTAAAATCAAAAACAAACCCGGGAGAACCCAATGACTGCACCCGCTCATACCCCGGCACCAACaca < 2:65641/147‑3 (MQ=255) cccTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACAGAcc < 3:285797/149‑1 (MQ=255) cTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACAGACCAt < 1:125254/149‑1 (MQ=255) cTTGCACAATCGACAATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACAGACCAt > 5:197231/1‑149 (MQ=255) ttGCACAATCGACAATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACAGACCATg > 7:181624/1‑149 (MQ=255) cacaATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCaa < 6:363624/137‑1 (MQ=255) cacaATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCaa > 5:363624/1‑137 (MQ=255) cacaATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACAGACCATGTa < 3:250431/148‑1 (MQ=255) | AACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACAGACCATGTA > NC_002947/3960465‑3960754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |