Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,960,617 G→A 7.0% intergenic (‑233/‑96) mnxG ← / → PP_3491 manganese‑oxidizing multicopper oxidase/enoly‑coenzyme A hydratase/isomerase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,960,6170GA 7.0% 162.9 / 2.2 57intergenic (‑233/‑96)mnxG/PP_3491manganese‑oxidizing multicopper oxidase/enoly‑coenzyme A hydratase/isomerase family protein
Reads supporting (aligned to +/- strand):  ref base G (26/27);  new base A (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.67e-01

CGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACAGACCATGTA  >  NC_002947/3960473‑3960754
                                                                                                                                                |                                                                                                                                         
cGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATtg                                                                                                                                       >  1:65641/1‑149 (MQ=255)
    cAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGc                                                                                                                                    >  1:91976/1‑148 (MQ=255)
     aaCCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATCGACAATTGTGCaa                                                                                                                                  <  8:39243/149‑1 (MQ=255)
     aaCCGCAACGGATTGGGGAGGATACCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATCGACAATTGTGCa                                                                                                                                   <  4:211368/148‑1 (MQ=255)
          cAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAg                                                                                                                                 <  5:360020/145‑1 (MQ=255)
          cAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAg                                                                                                                                 >  6:360020/1‑145 (MQ=255)
               gATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCg                                                                                                                        >  2:125556/1‑149 (MQ=255)
                 ttGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCa                                                                                                                      <  8:280582/149‑1 (MQ=255)
                    gggAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGc                                                                                                                   >  4:285797/1‑149 (MQ=255)
                     ggaggaTCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGcc                                                                                                                  <  3:9373/149‑1 (MQ=255)
                           tCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAgg                                                                                                                                >  2:217399/1‑129 (MQ=255)
                           tCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAgg                                                                                                                                <  1:217399/129‑1 (MQ=255)
                              cccAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGa                                                                                                              >  5:48085/1‑144 (MQ=255)
                              cccAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGa                                                                                                              <  6:48085/144‑1 (MQ=255)
                              cccAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTg                                                                                                         <  8:137781/149‑1 (MQ=255)
                                  gcagGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGAt                                                                                                     >  1:78261/1‑149 (MQ=255)
                                      gTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGAtcctc                                                                                                 >  6:261855/1‑149 (MQ=255)
                                       ttCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTg                                                                                                         >  5:374693/1‑140 (MQ=255)
                                       ttCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTg                                                                                                         <  6:374693/140‑1 (MQ=255)
                                            ccGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCg                                                                                            >  8:94041/1‑148 (MQ=255)
                                            ccGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCg                                                                                            >  4:222993/1‑148 (MQ=255)
                                            ccGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCCATCCTCCAGCg                                                                                            >  3:26145/1‑148 (MQ=255)
                                                    ggggACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGa                                                                                   <  5:261855/149‑1 (MQ=255)
                                                            aacctgctGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACtt                                                                           <  4:15898/142‑1 (MQ=255)
                                                              tCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTg                                                                          >  2:125254/1‑148 (MQ=255)
                                                                           aaGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAAc                                                             >  1:339206/1‑148 (MQ=255)
                                                                           aaGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACa                                                            <  2:78261/149‑1 (MQ=255)
                                                                                  ctCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAgg                                                     <  2:91976/149‑1 (MQ=255)
                                                                                   tCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAgg                                                     >  4:250431/1‑148 (MQ=255)
                                                                                   tCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAgg                                                     >  6:174795/1‑148 (MQ=255)
                                                                                     cacaAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCACCGAACTCAACCAATCACTTGGAAAATCAAAAACAAACCAGTgag                                                  >  8:322105/1‑149 (MQ=255)
                                                                                        aaGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCt                                                                                                          <  7:293648/90‑1 (MQ=255)
                                                                                        aaGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCt                                                                                                          >  8:293648/1‑90 (MQ=255)
                                                                                               cgcTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGa                                        <  3:222993/149‑1 (MQ=255)
                                                                                               cgcTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGa                                        <  1:240789/149‑1 (MQ=255)
                                                                                                gcTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCATAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGAc                                       <  1:125556/149‑1 (MQ=255)
                                                                                                    gCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGca                                   <  4:224778/149‑1 (MQ=255)
                                                                                                         acacaAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCAAGCGATCATCCAGCGAACTCAAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGcaccag                               >  3:66529/1‑145 (MQ=255)
                                                                                                            caAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCa                           <  7:94041/149‑1 (MQ=255)
                                                                                                             aaGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCa                           >  3:187917/1‑148 (MQ=255)
                                                                                                               ggTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCa                                                                                     >  5:133600/1‑88 (MQ=255)
                                                                                                               ggTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCa                                                                                     <  6:133600/88‑1 (MQ=255)
                                                                                                               ggTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATAc                        >  5:100159/1‑149 (MQ=255)
                                                                                                                gTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACt                                      <  4:359454/134‑1 (MQ=255)
                                                                                                                gTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACt                                      >  3:359454/1‑134 (MQ=255)
                                                                                                                   aaaaCGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATAcccc                     <  3:117611/148‑1 (MQ=255)
                                                                                                                      aCGCACGGTACCCTTGCACAATTGTCGATTGTGCAATGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGgca                 <  7:322105/149‑1 (MQ=255)
                                                                                                                              tccccTTGCACATTTGTCGATTGTGAAAGGAAAATTCGCATGCCAGGATCCTGCGATCCTCCACCGAACTCCAGACTTCACTTGTAAAATCAAAAACAAACCCGGGAGAACCCAATGACTGCACCCGCTCATACCCCGGCACCAACaca         <  2:65641/147‑3 (MQ=255)
                                                                                                                                cccTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACAGAcc       <  3:285797/149‑1 (MQ=255)
                                                                                                                                  cTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACAGACCAt     <  1:125254/149‑1 (MQ=255)
                                                                                                                                  cTTGCACAATCGACAATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACAGACCAt     >  5:197231/1‑149 (MQ=255)
                                                                                                                                   ttGCACAATCGACAATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACAGACCATg    >  7:181624/1‑149 (MQ=255)
                                                                                                                                      cacaATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCaa             <  6:363624/137‑1 (MQ=255)
                                                                                                                                      cacaATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCaa             >  5:363624/1‑137 (MQ=255)
                                                                                                                                      cacaATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACAGACCATGTa  <  3:250431/148‑1 (MQ=255)
                                                                                                                                             gTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACa           <  1:124811/132‑1 (MQ=255)
                                                                                                                                             gTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACa           >  2:124811/1‑132 (MQ=255)
                                                                                                                                                |                                                                                                                                         
CGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACAGACCATGTA  >  NC_002947/3960473‑3960754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑