Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,983,830 T→G 5.2% C282G (TGC→GGC)  ilvE → branched‑chain‑amino acid aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,983,8300TG 5.2% 234.5 / 2.1 73C282G (TGC→GGC) ilvEbranched‑chain‑amino acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (36/32);  new base G (2/2);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTA  >  NC_002947/3983692‑3983970
                                                                                                                                          |                                                                                                                                            
aCCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACcgc                                                                                                                                    >  3:332970/1‑149 (MQ=255)
 ccccGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACcgcg                                                                                                                                   <  7:352354/149‑1 (MQ=255)
 ccccGAAATCGGCCTCGGTACTGCCAGGCATAACCCGCCTCTCCCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGAGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGCCACagcg                                                                                                                                   >  2:373626/1‑149 (MQ=255)
  cccGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCgg                                                                                                                                  >  2:29989/1‑149 (MQ=255)
       aaTCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGtt                                                                                                                             <  8:97337/149‑1 (MQ=255)
            gCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCAc                                                                                                                        >  4:367536/1‑149 (MQ=255)
            gCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCGCAGGCACcggcttc                                                                                                                                <  1:207212/141‑6 (MQ=255)
            gCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCCGTGCCGCCGGCCCcggcttc                                                                                                                                >  2:207212/1‑136 (MQ=255)
                      tGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATcggc                                                                                                              >  1:361359/1‑149 (MQ=255)
                        ccAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGc                                                                                                                                            <  4:308771/117‑1 (MQ=255)
                        ccAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGc                                                                                                                                            >  3:308771/1‑117 (MQ=255)
                        ccAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATcggc                                                                                                              <  1:156160/147‑1 (MQ=255)
                              aTCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATc                                                                                                        >  1:124217/1‑147 (MQ=255)
                              aTCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATc                                                                                                        >  4:294188/1‑147 (MQ=255)
                              aTCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGa                                                                                                      <  7:195522/149‑1 (MQ=255)
                              aTCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCGCAGGCAcc                                                                                                                                      <  5:198672/117‑1 (MQ=255)
                              aTCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCGCAGGCAcc                                                                                                                                      >  6:198672/1‑117 (MQ=255)
                               tCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAg                                                                                                     <  2:361359/149‑1 (MQ=255)
                                 aCCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCAGGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTa                                                                                                   <  4:234901/149‑1 (MQ=255)
                                   ccGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTAc                                                                                                  >  3:181708/1‑148 (MQ=255)
                                   ccGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTAc                                                                                                  >  6:67922/1‑148 (MQ=255)
                                          tcGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGCTCGGCGGCATCGAGTACAACGGc                                                                                            >  8:289303/1‑147 (MQ=255)
                                            gCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAg                                                                                         <  4:141584/148‑1 (MQ=255)
                                                aTGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGc                                                                                     <  8:168830/148‑1 (MQ=255)
                                                     aCTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATcggc                                                                                                              <  2:238060/118‑1 (MQ=255)
                                                     aCTGGCGCAATCGCGCCTGGGCATGACCGTGAACGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATcggc                                                                                                              >  1:238060/1‑118 (MQ=255)
                                                         gcgcAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCTTCGAGTACAACGGCAAGCTGCACGTGTTcc                                                                            <  1:29989/148‑1 (MQ=255)
                                                         gcgcAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTcc                                                                            <  3:256162/148‑1 (MQ=255)
                                                           gcAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTAACACCCCCAACGGCGGCAACGAGTACAACGGCAAGCAGCACGTGTTCCACg                                                                         >  6:260939/1‑149 (MQ=255)
                                                             aaTCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATc                                                                                                        >  6:373343/1‑116 (MQ=255)
                                                                        ggCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAg                                                              <  8:48627/147‑1 (MQ=255)
                                                                        ggCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGt                                                            >  5:93116/1‑149 (MQ=255)
                                                                         gCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTc                                                           <  4:306661/149‑1 (MQ=255)
                                                                               ccGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCg                                                     <  3:294188/149‑1 (MQ=255)
                                                                                cGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCg                                                          >  6:334945/1‑143 (MQ=255)
                                                                                cGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCg                                                          <  5:334945/143‑1 (MQ=255)
                                                                                 gTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGt                                                   >  7:299132/1‑149 (MQ=255)
                                                                                   gATCGAAGGCGATGTCGAGATCAGCAAGCTGAACCGCTTCGACGAAGCCGATAAAAGCGGCACCCCGGCCGTTAAAACCCCGATAGGAGGCAAAGAGTACAACGGCAACGGGAAGGTGTGCCCCGCCGGGGAAAAGGGCGCCCCGGtga                                                 >  8:239861/1‑147 (MQ=255)
                                                                                    aTCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTc                                                                             <  8:225000/120‑1 (MQ=255)
                                                                                    aTCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTc                                                                             >  7:225000/1‑120 (MQ=255)
                                                                                    aTCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTAc                                                <  6:93116/149‑1 (MQ=255)
                                                                                       gAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTAcc                                               >  5:28090/1‑147 (MQ=255)
                                                                                       gAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTAcc                                               <  6:28090/147‑1 (MQ=255)
                                                                                       gAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACAGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTAcc                                               >  6:195961/1‑147 (MQ=255)
                                                                                        aaGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTAcc                                               >  7:196556/1‑146 (MQ=255)
                                                                                        aaGGCGATGTCGAAATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCa                                                                           >  2:130955/1‑118 (MQ=255)
                                                                                           gCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATcggcg                                                                                                             <  8:234471/81‑1 (MQ=255)
                                                                                           gCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATcggcg                                                                                                             >  7:234471/1‑81 (MQ=255)
                                                                                           gCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAg                                         >  3:133525/1‑149 (MQ=255)
                                                                                             gATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGTTCGGCCCGGTTACCCAGAAGct                                       >  6:304730/1‑149 (MQ=255)
                                                                                             gATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCgg                                                         <  8:310510/131‑1 (MQ=255)
                                                                                             gATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGct                                       >  3:39460/1‑149 (MQ=255)
                                                                                              aTGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGctc                                      >  4:375425/1‑149 (MQ=255)
                                                                                                 tCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTa                                    <  8:299132/148‑1 (MQ=255)
                                                                                                 tCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTAc                                   <  2:124217/149‑1 (MQ=255)
                                                                                                        cAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCACAAGCTCaacaaccac                             >  8:326139/1‑145 (MQ=255)
                                                                                                          gCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGc                            <  6:41868/147‑1 (MQ=255)
                                                                                                          gCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGc                            >  5:41868/1‑147 (MQ=255)
                                                                                                           cAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGa                         >  8:226978/1‑149 (MQ=255)
                                                                                                           cAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGa                         >  4:29674/1‑149 (MQ=255)
                                                                                                            aaGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGAc                        <  3:375425/149‑1 (MQ=255)
                                                                                                                 ggACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAg                             <  8:293600/139‑1 (MQ=255)
                                                                                                                 ggACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAg                             >  7:293600/1‑139 (MQ=255)
                                                                                                                 ggACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACACCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGAACTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACAGGTa                   >  6:179658/1‑149 (MQ=255)
                                                                                                                  gACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGt                    <  4:181708/147‑1 (MQ=255)
                                                                                                                    ccGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAgg                                                             <  2:2356/104‑1 (MQ=255)
                                                                                                                    ccGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAgg                                                             >  1:2356/1‑104 (MQ=255)
                                                                                                                       cTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCa               >  5:208046/1‑147 (MQ=255)
                                                                                                                        ttCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCagag            >  4:224028/1‑149 (MQ=255)
                                                                                                                            tcgAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAgcggc        <  4:133525/149‑1 (MQ=255)
                                                                                                                              gAAGCCGGGGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAgcggcg       <  5:260939/148‑1 (MQ=255)
                                                                                                                               aaGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGAt     <  2:122715/149‑1 (MQ=255)
                                                                                                                                  ccGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTa  <  3:367536/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
ACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTA  >  NC_002947/3983692‑3983970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑