Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,049,523	C→T	6.0%	R692C (CGC→TGC)	quiA →	quinate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,049,523	0	C	T	6.0%	246.5 / 3.8	83	R692C (CGC→TGC)	quiA	quinate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (40/38);  new base T (3/2);  total (43/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-02

GTGGCGAAGCACTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACC  >  NC_002947/4049377‑4049669                                                                                                                                                   |                                                                                                                                                   gTGGCGAAGCACTGATCACTTGCATGGGCGCTGTACCGTTCAAGGGCAGCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGGACCGCCGTTTGGCACCCTGACCGGCTTCGACATGAAGACCCGc                                                                                                                                                  <  4:8763/149‑1 (MQ=255) gTGGCGAAGCACTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGc                                                                                                                                                  <  4:81669/149‑1 (MQ=255)     cGAAGCACTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAgg                                                                                                                                              >  7:186190/1‑149 (MQ=255)      gAAGCACTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGt                                                                                                                                             >  1:260232/1‑149 (MQ=255)      gAAGCACTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGt                                                                                                                                             >  6:111591/1‑149 (MQ=255)       aaGCACTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTg                                                                                                                                            >  2:161608/1‑149 (MQ=255)        aGCACTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGc                                                                                                                                                  <  4:338236/142‑1 (MQ=255)        aGCACTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGc                                                                                                                                                  >  3:338236/1‑142 (MQ=255)           aCTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCt                                                                                                                                        >  5:337498/1‑149 (MQ=255)            cTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTg                                                                                                                                       <  1:161608/149‑1 (MQ=255)               aaCACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTgg                                                                                                                                      >  4:268876/1‑147 (MQ=255)               aaCACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGc                                                                                                                                     <  8:356367/148‑1 (MQ=255)                acacTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCa                                                                                                                                    <  7:145187/148‑1 (MQ=255)                  acTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGt                                                                                                                                 <  1:330653/149‑1 (MQ=255)                      gCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGc                                                                                                                               <  8:186190/147‑1 (MQ=255)                         tGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTa                                                                                                                          <  3:268876/149‑1 (MQ=255)                         tGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTa                                                                                                                          <  8:236097/149‑1 (MQ=255)                          gggCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCgg                                                                                                                            >  6:139170/1‑146 (MQ=255)                          gggCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTa                                                                                                                          <  5:139170/148‑1 (MQ=255)                          gggCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAg                                                                                                                         >  8:259629/1‑149 (MQ=255)                          gggCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAg                                                                                                                         >  6:36558/1‑149 (MQ=255)                            gcgcGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAgg                                                                                                                        >  8:21953/1‑148 (MQ=255)                                 gTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGt                                                                                                                  >  6:86682/1‑149 (MQ=255)                                  tACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGtt                                                                                                                 <  6:337498/149‑1 (MQ=255)                                     cGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGaa                                                                                                              <  5:111591/149‑1 (MQ=255)                                     cGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGaa                                                                                                              <  8:239555/149‑1 (MQ=255)                                       cTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCTGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGaa                                                                                                              <  6:350210/147‑1 (MQ=255)                                          aaGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGacac                                                                                                         >  1:14626/1‑149 (MQ=255)                                          aaGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGaca                                                                                                          <  7:21953/148‑1 (MQ=255)                                            gggCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCg                                                                                                                             >  8:258050/1‑127 (MQ=255)                                            gggCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCg                                                                                                                             <  7:258050/127‑1 (MQ=255)                                            gggCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCg                                                                                                       >  3:297155/1‑149 (MQ=255)                                            gggCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCg                                                                                                       <  7:31696/149‑1 (MQ=255)                                              gCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGc                                                                                                     >  5:350632/1‑149 (MQ=255)                                                  cccGTATGCCTTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGc                                                                                                 <  8:55401/149‑1 (MQ=255)                                                  cccGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGc                                                                                                 >  3:284730/1‑149 (MQ=255)                                                          ccGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCACGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCgg                                                                                             <  6:318903/145‑1 (MQ=255)                                                          ccGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCACGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCgg                                                                                             >  5:318903/1‑145 (MQ=255)                                                           cGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATcc                                                                                        >  5:277552/1‑149 (MQ=255)                                                               acccacaaCCTCTTCCTGCCGGGCGCCGGCATTCCACTCCAGGCACCCCCGGTTGGCACCCTGACCGCCATCCACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCAt                                                                                    <  4:284730/143‑1 (MQ=255)                                                                 cAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATg                                                                                   >  4:145506/1‑148 (MQ=255)                                                                   aGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGc                                                                                                                                     >  2:84067/1‑96 (MQ=255)                                                                   aGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGc                                                                                                                                     <  1:84067/96‑1 (MQ=255)                                                                          cTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGa                                                                         <  1:189821/149‑1 (MQ=255)                                                                          cTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGa                                                                         <  5:36558/149‑1 (MQ=255)                                                                          cTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGa                                                                         <  1:63125/149‑1 (MQ=255)                                                                              cTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCa                                                                      <  3:145506/148‑1 (MQ=255)                                                                              cTGTCGGTCGCCGGAATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCa                                                                      <  7:168422/148‑1 (MQ=255)                                                                                       gCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCTTGATCGGca                                                             <  5:86682/148‑2 (MQ=255)                                                                                       gCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCt                                                            >  4:8621/1‑149 (MQ=255)                                                                                       gCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCt                                                            >  3:274688/1‑149 (MQ=255)                                                                                       gCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCt                                                            <  2:14626/149‑1 (MQ=255)                                                                                         cGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCTTGATCGGcaggt                                                          <  2:260232/149‑5 (MQ=255)                                                                                                  aTGCCAGGCACCGCCGTTTGGCACCCTGCCCGCCATCGACATGAAGACCCGCCCGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTg                                                 >  5:209203/1‑149 (MQ=255)                                                                                                  aTGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTg                                                 >  5:214478/1‑149 (MQ=255)                                                                                                     ccAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTggcg                                              >  8:178556/1‑149 (MQ=255)                                                                                                     ccAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGAGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTggcg                                              >  7:17125/1‑149 (MQ=255)                                                                                                       aGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGACAACCCTTggcgg                                             >  5:93469/1‑148 (MQ=255)                                                                                                             cgccgTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTg                                      >  3:207778/1‑149 (MQ=255)                                                                                                             cgccgTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTg                                      >  2:364997/1‑149 (MQ=255)                                                                                                                    tGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGt                                                                                                                  <  3:150674/66‑1 (MQ=255)                                                                                                                    tGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGt                                                                                                                  >  4:150674/1‑66 (MQ=255)                                                                                                                      gCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGAccc                               >  2:234356/1‑147 (MQ=255)                                                                                                                        aCCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAgg                           >  6:113228/1‑149 (MQ=255)                                                                                                                        aCCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAgg                           <  5:113228/149‑1 (MQ=255)                                                                                                                            tGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACAACGGCCCGTTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAggcggc                       >  1:100254/1‑149 (MQ=255)                                                                                                                                cGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTgg                   <  4:207778/149‑1 (MQ=255)                                                                                                                                 gCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGgt                  <  3:31462/149‑1 (MQ=255)                                                                                                                                 gCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGgt                  <  3:36395/149‑1 (MQ=255)                                                                                                                                       gACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCAt            >  4:6353/1‑149 (MQ=255)                                                                                                                                            gAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg       >  3:202286/1‑149 (MQ=255)                                                                                                                                            gAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg       >  3:172720/1‑149 (MQ=255)                                                                                                                                            gAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg       >  1:268628/1‑149 (MQ=255)                                                                                                                                            gAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg       >  1:78686/1‑149 (MQ=255)                                                                                                                                            cggcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGTGGCACCCTGTCGa                                  <  3:373175/118‑1 (MQ=255)                                                                                                                                            cggcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGTGGCACCCTGTCGa                                  >  4:373175/5‑122 (MQ=255)                                                                                                                                             ggcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGgtgt                >  4:90300/4‑139 (MQ=255)                                                                                                                                             ggcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGgtgt                <  3:90300/136‑1 (MQ=255)                                                                                                                                             aaGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCAGCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAgg      >  7:217351/1‑149 (MQ=255)                                                                                                                                             aaGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg       <  3:8621/148‑1 (MQ=255)                                                                                                                                             aaGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg       <  7:178556/148‑1 (MQ=255)                                                                                                                                              gcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAgg      >  7:239532/3‑148 (MQ=255)                                                                                                                                              aGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGt     <  4:274688/149‑1 (MQ=255)                                                                                                                                                  ccGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTAcc  >  3:190908/1‑148 (MQ=255)                                                                                                                                                  ccGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTAcc  >  5:193329/1‑148 (MQ=255)                                                                                                                                                   cGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACCCCGGCCCGCTCGGCATCCGCATGCACCTGCCGAGCAAGATCGGCCTGCCACCCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTAcc  >  3:377388/1‑147 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GTGGCGAAGCACTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACC  >  NC_002947/4049377‑4049669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑