Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,049,530 T→C 7.4% V694A (GTG→GCG) ‡ quiA → quinate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,049,5300TC 7.4% 231.3 / 7.2 82V694A (GTG→GCG) ‡quiAquinate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (39/36);  new base C (4/2);  total (44/38)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.52e-01

GAAGCACTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAG  >  NC_002947/4049382‑4049672
                                                                                                                                                    |                                                                                                                                              
gAAGCACTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGt                                                                                                                                                >  6:111591/1‑149 (MQ=255)
gAAGCACTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGt                                                                                                                                                >  1:260232/1‑149 (MQ=255)
 aaGCACTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTg                                                                                                                                               >  2:161608/1‑149 (MQ=255)
     aCTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCt                                                                                                                                           >  5:337498/1‑149 (MQ=255)
      cTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTg                                                                                                                                          <  1:161608/149‑1 (MQ=255)
         aaCACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTgg                                                                                                                                         >  4:268876/1‑147 (MQ=255)
         aaCACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGc                                                                                                                                        <  8:356367/148‑1 (MQ=255)
          acacTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCa                                                                                                                                       <  7:145187/148‑1 (MQ=255)
            acTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGt                                                                                                                                    <  1:330653/149‑1 (MQ=255)
                gCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGc                                                                                                                                  <  8:186190/147‑1 (MQ=255)
                   tGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTa                                                                                                                             <  8:236097/149‑1 (MQ=255)
                   tGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTa                                                                                                                             <  3:268876/149‑1 (MQ=255)
                    gggCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCgg                                                                                                                               >  6:139170/1‑146 (MQ=255)
                    gggCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTa                                                                                                                             <  5:139170/148‑1 (MQ=255)
                    gggCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAg                                                                                                                            >  8:259629/1‑149 (MQ=255)
                    gggCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAg                                                                                                                            >  6:36558/1‑149 (MQ=255)
                      gcgcGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAgg                                                                                                                           >  8:21953/1‑148 (MQ=255)
                           gTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGt                                                                                                                     >  6:86682/1‑149 (MQ=255)
                            tACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGtt                                                                                                                    <  6:337498/149‑1 (MQ=255)
                               cGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGaa                                                                                                                 <  5:111591/149‑1 (MQ=255)
                               cGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGaa                                                                                                                 <  8:239555/149‑1 (MQ=255)
                                 cTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCTGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGaa                                                                                                                 <  6:350210/147‑1 (MQ=255)
                                    aaGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGacac                                                                                                            >  1:14626/1‑149 (MQ=255)
                                    aaGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGaca                                                                                                             <  7:21953/148‑1 (MQ=255)
                                      gggCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCg                                                                                                                                <  7:258050/127‑1 (MQ=255)
                                      gggCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCg                                                                                                                                >  8:258050/1‑127 (MQ=255)
                                      gggCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCg                                                                                                          <  7:31696/149‑1 (MQ=255)
                                      gggCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCg                                                                                                          >  3:297155/1‑149 (MQ=255)
                                        gCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGc                                                                                                        >  5:350632/1‑149 (MQ=255)
                                            cccGTATGCCTTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGc                                                                                                    <  8:55401/149‑1 (MQ=255)
                                            cccGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGc                                                                                                    >  3:284730/1‑149 (MQ=255)
                                                    ccGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCACGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCgg                                                                                                <  6:318903/145‑1 (MQ=255)
                                                    ccGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCACGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCgg                                                                                                >  5:318903/1‑145 (MQ=255)
                                                     cGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATcc                                                                                           >  5:277552/1‑149 (MQ=255)
                                                         acccacaaCCTCTTCCTGCCGGGCGCCGGCATTCCACTCCAGGCACCCCCGGTTGGCACCCTGACCGCCATCCACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCAt                                                                                       <  4:284730/143‑1 (MQ=255)
                                                           cAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATg                                                                                      >  4:145506/1‑148 (MQ=255)
                                                             aGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGc                                                                                                                                        >  2:84067/1‑96 (MQ=255)
                                                             aGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGc                                                                                                                                        <  1:84067/96‑1 (MQ=255)
                                                                    cTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGa                                                                            <  1:189821/149‑1 (MQ=255)
                                                                    cTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGa                                                                            <  1:63125/149‑1 (MQ=255)
                                                                    cTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGa                                                                            <  5:36558/149‑1 (MQ=255)
                                                                        cTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCa                                                                         <  3:145506/148‑1 (MQ=255)
                                                                        cTGTCGGTCGCCGGAATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCa                                                                         <  7:168422/148‑1 (MQ=255)
                                                                                 gCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCTTGATCGGca                                                                <  5:86682/148‑2 (MQ=255)
                                                                                 gCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCt                                                               >  4:8621/1‑149 (MQ=255)
                                                                                 gCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCt                                                               >  3:274688/1‑149 (MQ=255)
                                                                                 gCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCt                                                               <  2:14626/149‑1 (MQ=255)
                                                                                   cGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCTTGATCGGcaggt                                                             <  2:260232/149‑5 (MQ=255)
                                                                                            aTGCCAGGCACCGCCGTTTGGCACCCTGCCCGCCATCGACATGAAGACCCGCCCGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTg                                                    >  5:209203/1‑149 (MQ=255)
                                                                                            aTGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTg                                                    >  5:214478/1‑149 (MQ=255)
                                                                                               ccAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTggcg                                                 >  8:178556/1‑149 (MQ=255)
                                                                                               ccAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGAGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTggcg                                                 >  7:17125/1‑149 (MQ=255)
                                                                                                 aGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGACAACCCTTggcgg                                                >  5:93469/1‑148 (MQ=255)
                                                                                                       cgccgTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTg                                         >  3:207778/1‑149 (MQ=255)
                                                                                                       cgccgTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTg                                         >  2:364997/1‑149 (MQ=255)
                                                                                                              tGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGt                                                                                                                     >  4:150674/1‑66 (MQ=255)
                                                                                                              tGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGt                                                                                                                     <  3:150674/66‑1 (MQ=255)
                                                                                                                gCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGAccc                                  >  2:234356/1‑147 (MQ=255)
                                                                                                                  aCCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAgg                              <  5:113228/149‑1 (MQ=255)
                                                                                                                  aCCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAgg                              >  6:113228/1‑149 (MQ=255)
                                                                                                                      tGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACAACGGCCCGTTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAggcggc                          >  1:100254/1‑149 (MQ=255)
                                                                                                                          cGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTgg                      <  4:207778/149‑1 (MQ=255)
                                                                                                                           gCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGgt                     <  3:36395/149‑1 (MQ=255)
                                                                                                                           gCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGgt                     <  3:31462/149‑1 (MQ=255)
                                                                                                                                 gACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCAt               >  4:6353/1‑149 (MQ=255)
                                                                                                                                      gAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg          >  1:78686/1‑149 (MQ=255)
                                                                                                                                      gAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg          >  1:268628/1‑149 (MQ=255)
                                                                                                                                      gAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg          >  3:202286/1‑149 (MQ=255)
                                                                                                                                      gAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg          >  3:172720/1‑149 (MQ=255)
                                                                                                                                      cggcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGTGGCACCCTGTCGa                                     <  3:373175/118‑1 (MQ=255)
                                                                                                                                      cggcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGTGGCACCCTGTCGa                                     >  4:373175/5‑122 (MQ=255)
                                                                                                                                       ggcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGgtgt                   >  4:90300/4‑139 (MQ=255)
                                                                                                                                       ggcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGgtgt                   <  3:90300/136‑1 (MQ=255)
                                                                                                                                       aaGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCAGCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAgg         >  7:217351/1‑149 (MQ=255)
                                                                                                                                       aaGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg          <  7:178556/148‑1 (MQ=255)
                                                                                                                                       aaGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg          <  3:8621/148‑1 (MQ=255)
                                                                                                                                        gcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAgg         >  7:239532/3‑148 (MQ=255)
                                                                                                                                        aGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGt        <  4:274688/149‑1 (MQ=255)
                                                                                                                                            ccGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTAcc     >  3:190908/1‑148 (MQ=255)
                                                                                                                                            ccGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTAcc     >  5:193329/1‑148 (MQ=255)
                                                                                                                                             cGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACCCCGGCCCGCTCGGCATCCGCATGCACCTGCCGAGCAAGATCGGCCTGCCACCCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTAcc     >  3:377388/1‑147 (MQ=255)
                                                                                                                                              gCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTagaagt  >  1:87471/1‑144 (MQ=255)
                                                                                                                                              gCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAg  <  2:78686/149‑1 (MQ=255)
                                                                                                                                              gCCAGGCGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAg  >  6:158553/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
GAAGCACTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAG  >  NC_002947/4049382‑4049672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑