Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,049,532	G→A	6.1%	A695T (GCC→ACC)	quiA →	quinate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,049,532	0	G	A	6.1%	246.9 / 3.9	81	A695T (GCC→ACC)	quiA	quinate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (40/36);  new base A (3/2);  total (43/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.92e-01

ACTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAG  >  NC_002947/4049387‑4049672                                                                                                                                                  |                                                                                                                                             aCTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCt                                                                                                                                           >  5:337498/1‑149 (MQ=255)  cTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTg                                                                                                                                          <  1:161608/149‑1 (MQ=255)     aaCACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTgg                                                                                                                                         >  4:268876/1‑147 (MQ=255)     aaCACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGc                                                                                                                                        <  8:356367/148‑1 (MQ=255)      acacTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCa                                                                                                                                       <  7:145187/148‑1 (MQ=255)        acTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGt                                                                                                                                    <  1:330653/149‑1 (MQ=255)            gCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGc                                                                                                                                  <  8:186190/147‑1 (MQ=255)               tGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTa                                                                                                                             <  8:236097/149‑1 (MQ=255)               tGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTa                                                                                                                             <  3:268876/149‑1 (MQ=255)                gggCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCgg                                                                                                                               >  6:139170/1‑146 (MQ=255)                gggCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTa                                                                                                                             <  5:139170/148‑1 (MQ=255)                gggCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAg                                                                                                                            >  8:259629/1‑149 (MQ=255)                gggCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAg                                                                                                                            >  6:36558/1‑149 (MQ=255)                  gcgcGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAgg                                                                                                                           >  8:21953/1‑148 (MQ=255)                       gTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGt                                                                                                                     >  6:86682/1‑149 (MQ=255)                        tACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGtt                                                                                                                    <  6:337498/149‑1 (MQ=255)                           cGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGaa                                                                                                                 <  8:239555/149‑1 (MQ=255)                           cGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGaa                                                                                                                 <  5:111591/149‑1 (MQ=255)                             cTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCTGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGaa                                                                                                                 <  6:350210/147‑1 (MQ=255)                                aaGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGacac                                                                                                            >  1:14626/1‑149 (MQ=255)                                aaGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGaca                                                                                                             <  7:21953/148‑1 (MQ=255)                                  gggCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCg                                                                                                                                <  7:258050/127‑1 (MQ=255)                                  gggCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCg                                                                                                                                >  8:258050/1‑127 (MQ=255)                                  gggCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCg                                                                                                          <  7:31696/149‑1 (MQ=255)                                  gggCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCg                                                                                                          >  3:297155/1‑149 (MQ=255)                                    gCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGc                                                                                                        >  5:350632/1‑149 (MQ=255)                                        cccGTATGCCTTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGc                                                                                                    <  8:55401/149‑1 (MQ=255)                                        cccGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGc                                                                                                    >  3:284730/1‑149 (MQ=255)                                                ccGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCACGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCgg                                                                                                <  6:318903/145‑1 (MQ=255)                                                ccGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCACGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCgg                                                                                                >  5:318903/1‑145 (MQ=255)                                                 cGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATcc                                                                                           >  5:277552/1‑149 (MQ=255)                                                     acccacaaCCTCTTCCTGCCGGGCGCCGGCATTCCACTCCAGGCACCCCCGGTTGGCACCCTGACCGCCATCCACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCAt                                                                                       <  4:284730/143‑1 (MQ=255)                                                       cAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATg                                                                                      >  4:145506/1‑148 (MQ=255)                                                         aGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGc                                                                                                                                        <  1:84067/96‑1 (MQ=255)                                                         aGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGc                                                                                                                                        >  2:84067/1‑96 (MQ=255)                                                                cTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGa                                                                            <  5:36558/149‑1 (MQ=255)                                                                cTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGa                                                                            <  1:189821/149‑1 (MQ=255)                                                                cTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGa                                                                            <  1:63125/149‑1 (MQ=255)                                                                    cTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCa                                                                         <  3:145506/148‑1 (MQ=255)                                                                    cTGTCGGTCGCCGGAATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCa                                                                         <  7:168422/148‑1 (MQ=255)                                                                             gCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCTTGATCGGca                                                                <  5:86682/148‑2 (MQ=255)                                                                             gCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCt                                                               <  2:14626/149‑1 (MQ=255)                                                                             gCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCt                                                               >  3:274688/1‑149 (MQ=255)                                                                             gCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCt                                                               >  4:8621/1‑149 (MQ=255)                                                                               cGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCTTGATCGGcaggt                                                             <  2:260232/149‑5 (MQ=255)                                                                                        aTGCCAGGCACCGCCGTTTGGCACCCTGCCCGCCATCGACATGAAGACCCGCCCGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTg                                                    >  5:209203/1‑149 (MQ=255)                                                                                        aTGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTg                                                    >  5:214478/1‑149 (MQ=255)                                                                                           ccAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTggcg                                                 >  8:178556/1‑149 (MQ=255)                                                                                           ccAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGAGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTggcg                                                 >  7:17125/1‑149 (MQ=255)                                                                                             aGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGACAACCCTTggcgg                                                >  5:93469/1‑148 (MQ=255)                                                                                                   cgccgTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTg                                         >  2:364997/1‑149 (MQ=255)                                                                                                   cgccgTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTg                                         >  3:207778/1‑149 (MQ=255)                                                                                                          tGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGt                                                                                                                     >  4:150674/1‑66 (MQ=255)                                                                                                          tGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGt                                                                                                                     <  3:150674/66‑1 (MQ=255)                                                                                                            gCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGAccc                                  >  2:234356/1‑147 (MQ=255)                                                                                                              aCCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAgg                              <  5:113228/149‑1 (MQ=255)                                                                                                              aCCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAgg                              >  6:113228/1‑149 (MQ=255)                                                                                                                  tGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACAACGGCCCGTTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAggcggc                          >  1:100254/1‑149 (MQ=255)                                                                                                                      cGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTgg                      <  4:207778/149‑1 (MQ=255)                                                                                                                       gCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGgt                     <  3:36395/149‑1 (MQ=255)                                                                                                                       gCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGgt                     <  3:31462/149‑1 (MQ=255)                                                                                                                             gACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCAt               >  4:6353/1‑149 (MQ=255)                                                                                                                                  gAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg          >  3:172720/1‑149 (MQ=255)                                                                                                                                  gAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg          >  1:268628/1‑149 (MQ=255)                                                                                                                                  gAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg          >  1:78686/1‑149 (MQ=255)                                                                                                                                  gAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg          >  3:202286/1‑149 (MQ=255)                                                                                                                                  cggcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGTGGCACCCTGTCGa                                     >  4:373175/5‑122 (MQ=255)                                                                                                                                  cggcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGTGGCACCCTGTCGa                                     <  3:373175/118‑1 (MQ=255)                                                                                                                                   ggcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGgtgt                   >  4:90300/4‑139 (MQ=255)                                                                                                                                   ggcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGgtgt                   <  3:90300/136‑1 (MQ=255)                                                                                                                                   aaGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCAGCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAgg         >  7:217351/1‑149 (MQ=255)                                                                                                                                   aaGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg          <  7:178556/148‑1 (MQ=255)                                                                                                                                   aaGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg          <  3:8621/148‑1 (MQ=255)                                                                                                                                    gcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAgg         >  7:239532/3‑148 (MQ=255)                                                                                                                                    aGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGt        <  4:274688/149‑1 (MQ=255)                                                                                                                                        ccGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTAcc     >  5:193329/1‑148 (MQ=255)                                                                                                                                        ccGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTAcc     >  3:190908/1‑148 (MQ=255)                                                                                                                                         cGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACCCCGGCCCGCTCGGCATCCGCATGCACCTGCCGAGCAAGATCGGCCTGCCACCCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTAcc     >  3:377388/1‑147 (MQ=255)                                                                                                                                          gCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTagaagt  >  1:87471/1‑144 (MQ=255)                                                                                                                                          gCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAg  <  2:78686/149‑1 (MQ=255)                                                                                                                                          gCCAGGCGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAg  >  6:158553/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                             ACTGAACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAG  >  NC_002947/4049387‑4049672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑