Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,049,539 A→G 6.3% Q697R (CAG→CGG)  quiA → quinate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,049,5390AG 6.3% 236.0 / 3.6 79Q697R (CAG→CGG) quiAquinate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (39/35);  new base G (3/2);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGC  >  NC_002947/4049392‑4049687
                                                                                                                                                   |                                                                                                                                                    
acacTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCa                                                                                                                                                      <  7:145187/148‑1 (MQ=255)
  acTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGt                                                                                                                                                   <  1:330653/149‑1 (MQ=255)
      gCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGc                                                                                                                                                 <  8:186190/147‑1 (MQ=255)
         tGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTa                                                                                                                                            <  8:236097/149‑1 (MQ=255)
         tGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTa                                                                                                                                            <  3:268876/149‑1 (MQ=255)
          gggCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCgg                                                                                                                                              >  6:139170/1‑146 (MQ=255)
          gggCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTa                                                                                                                                            <  5:139170/148‑1 (MQ=255)
          gggCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAg                                                                                                                                           >  6:36558/1‑149 (MQ=255)
          gggCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAg                                                                                                                                           >  8:259629/1‑149 (MQ=255)
            gcgcGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAgg                                                                                                                                          >  8:21953/1‑148 (MQ=255)
                 gTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGt                                                                                                                                    >  6:86682/1‑149 (MQ=255)
                  tACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGtt                                                                                                                                   <  6:337498/149‑1 (MQ=255)
                     cGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGaa                                                                                                                                <  8:239555/149‑1 (MQ=255)
                     cGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGaa                                                                                                                                <  5:111591/149‑1 (MQ=255)
                       cTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCTGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGaa                                                                                                                                <  6:350210/147‑1 (MQ=255)
                          aaGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGacac                                                                                                                           >  1:14626/1‑149 (MQ=255)
                          aaGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGaca                                                                                                                            <  7:21953/148‑1 (MQ=255)
                            gggCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCg                                                                                                                                               <  7:258050/127‑1 (MQ=255)
                            gggCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCg                                                                                                                                               >  8:258050/1‑127 (MQ=255)
                            gggCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCg                                                                                                                         <  7:31696/149‑1 (MQ=255)
                            gggCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCg                                                                                                                         >  3:297155/1‑149 (MQ=255)
                              gCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGc                                                                                                                       >  5:350632/1‑149 (MQ=255)
                                  cccGTATGCCTTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGc                                                                                                                   <  8:55401/149‑1 (MQ=255)
                                  cccGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGc                                                                                                                   >  3:284730/1‑149 (MQ=255)
                                          ccGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCACGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCgg                                                                                                               <  6:318903/145‑1 (MQ=255)
                                          ccGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCACGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCgg                                                                                                               >  5:318903/1‑145 (MQ=255)
                                           cGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATcc                                                                                                          >  5:277552/1‑149 (MQ=255)
                                               acccacaaCCTCTTCCTGCCGGGCGCCGGCATTCCACTCCAGGCACCCCCGGTTGGCACCCTGACCGCCATCCACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCAt                                                                                                      <  4:284730/143‑1 (MQ=255)
                                                 cAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATg                                                                                                     >  4:145506/1‑148 (MQ=255)
                                                          cTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGa                                                                                           <  1:63125/149‑1 (MQ=255)
                                                          cTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGa                                                                                           <  5:36558/149‑1 (MQ=255)
                                                          cTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGa                                                                                           <  1:189821/149‑1 (MQ=255)
                                                              cTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCa                                                                                        <  3:145506/148‑1 (MQ=255)
                                                              cTGTCGGTCGCCGGAATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCa                                                                                        <  7:168422/148‑1 (MQ=255)
                                                                       gCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCTTGATCGGca                                                                               <  5:86682/148‑2 (MQ=255)
                                                                       gCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCt                                                                              <  2:14626/149‑1 (MQ=255)
                                                                       gCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCt                                                                              >  3:274688/1‑149 (MQ=255)
                                                                       gCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCt                                                                              >  4:8621/1‑149 (MQ=255)
                                                                         cGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCTTGATCGGcaggt                                                                            <  2:260232/149‑5 (MQ=255)
                                                                                  aTGCCAGGCACCGCCGTTTGGCACCCTGCCCGCCATCGACATGAAGACCCGCCCGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTg                                                                   >  5:209203/1‑149 (MQ=255)
                                                                                  aTGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTg                                                                   >  5:214478/1‑149 (MQ=255)
                                                                                     ccAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTggcg                                                                >  8:178556/1‑149 (MQ=255)
                                                                                     ccAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGAGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTggcg                                                                >  7:17125/1‑149 (MQ=255)
                                                                                       aGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGACAACCCTTggcgg                                                               >  5:93469/1‑148 (MQ=255)
                                                                                             cgccgTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTg                                                        >  2:364997/1‑149 (MQ=255)
                                                                                             cgccgTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTg                                                        >  3:207778/1‑149 (MQ=255)
                                                                                                    tGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGt                                                                                                                                    <  3:150674/66‑1 (MQ=255)
                                                                                                    tGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGt                                                                                                                                    >  4:150674/1‑66 (MQ=255)
                                                                                                      gCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGAccc                                                 >  2:234356/1‑147 (MQ=255)
                                                                                                        aCCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAgg                                             <  5:113228/149‑1 (MQ=255)
                                                                                                        aCCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAgg                                             >  6:113228/1‑149 (MQ=255)
                                                                                                            tGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACAACGGCCCGTTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAggcggc                                         >  1:100254/1‑149 (MQ=255)
                                                                                                                cGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTgg                                     <  4:207778/149‑1 (MQ=255)
                                                                                                                 gCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGgt                                    <  3:36395/149‑1 (MQ=255)
                                                                                                                 gCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGgt                                    <  3:31462/149‑1 (MQ=255)
                                                                                                                       gACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCAt                              >  4:6353/1‑149 (MQ=255)
                                                                                                                            gAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg                         >  3:172720/1‑149 (MQ=255)
                                                                                                                            gAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg                         >  1:268628/1‑149 (MQ=255)
                                                                                                                            gAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg                         >  3:202286/1‑149 (MQ=255)
                                                                                                                            gAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg                         >  1:78686/1‑149 (MQ=255)
                                                                                                                            cggcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGTGGCACCCTGTCGa                                                    >  4:373175/5‑122 (MQ=255)
                                                                                                                            cggcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGTGGCACCCTGTCGa                                                    <  3:373175/118‑1 (MQ=255)
                                                                                                                             ggcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGgtgt                                  <  3:90300/136‑1 (MQ=255)
                                                                                                                             ggcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGgtgt                                  >  4:90300/4‑139 (MQ=255)
                                                                                                                             aaGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCAGCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAgg                        >  7:217351/1‑149 (MQ=255)
                                                                                                                             aaGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg                         <  7:178556/148‑1 (MQ=255)
                                                                                                                             aaGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAg                         <  3:8621/148‑1 (MQ=255)
                                                                                                                              gcaCCTGCCAGGCCACCTGGCGGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAgg                        >  7:239532/3‑148 (MQ=255)
                                                                                                                              aGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGt                       <  4:274688/149‑1 (MQ=255)
                                                                                                                                  ccGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTAcc                    >  5:193329/1‑148 (MQ=255)
                                                                                                                                  ccGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTAcc                    >  3:190908/1‑148 (MQ=255)
                                                                                                                                   cGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACCCCGGCCCGCTCGGCATCCGCATGCACCTGCCGAGCAAGATCGGCCTGCCACCCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTAcc                    >  3:377388/1‑147 (MQ=255)
                                                                                                                                    gCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTagaagt                 >  1:87471/1‑144 (MQ=255)
                                                                                                                                    gCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAg                 <  2:78686/149‑1 (MQ=255)
                                                                                                                                    gCCAGGCGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAg                 >  6:158553/1‑149 (MQ=255)
                                                                                                                                             ccTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTAc        <  6:350632/149‑1 (MQ=255)
                                                                                                                                             ccTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCAAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTa         >  7:307686/1‑148 (MQ=255)
                                                                                                                                                 gCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGAc              >  8:181910/1‑139 (MQ=255)
                                                                                                                                                 gCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGAc              <  7:181910/139‑1 (MQ=255)
                                                                                                                                                 gCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTgc    <  6:277552/149‑1 (MQ=255)
                                                                                                                                                   aGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGCCTTCTACCTgcgc  >  7:313435/1‑149 (MQ=255)
                                                                                                                                                   aGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTgcgc  <  7:259629/149‑1 (MQ=255)
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ACACTGGCATGGGCGCGGTACCGCTCAAGGGCACCCCGTATGCCGTGAACAAGAACCGCTTCCTGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGC  >  NC_002947/4049392‑4049687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑