Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,070,375 T→C 8.3% Q595R (CAG→CGG)  mdtB ← multidrug transporter membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,070,3750TC 8.3% 135.7 / 2.0 48Q595R (CAG→CGG) mdtBmultidrug transporter membrane protein
Reads supporting (aligned to +/- strand):  ref base T (26/18);  new base C (2/2);  total (28/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCCGGTCGACCTGCGGCTGCAGGCGGCTGATCACTTCGCTGGCGCTGACATCGCGCTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGGCGCTTCGGAAATACCCTGGATCACCCCGGTATCCTGCACCGGGAAGAAGCCCTTGGGCACGACCA  >  NC_002947/4070230‑4070519
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caGCCGGTCGACCTGCGGCTGCAGGCGGCTGATCACTTCGCTGGCGCTGACATCGCGCTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCa                                                                                                                                               >  5:353854/1‑149 (MQ=255)
caGCCGGTCGACCTGCGGCTGCAGGCGGCTGATCACTTCGCTGGCGCTGACATCGCGCTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCa                                                                                                                                               >  4:156495/1‑149 (MQ=255)
   ccGGTCGACCTGCGGCTGCAGGCGGCTGATCACTTCGCTGGCGCTGACATCGCGCTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACgg                                                                                                                                            >  2:328417/1‑149 (MQ=255)
              gCGGCTGCAGGCGGCTGATCACTTCGCTGGCGCTGACATCGCGCTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTg                                                                                                                                  >  6:163269/1‑148 (MQ=255)
                 gCTGCAGGCGGCTGATCACTTCGCTGGCGCTGACATCGCGCTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAgg                                                                                                                              <  1:328417/149‑1 (MQ=255)
                        gcggcTGATCACTTCGCTGGCGCTGACATCGCGCTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCt                                                                                                                       >  2:249207/1‑149 (MQ=255)
                               aTCACTTCGCTGGCGCTGACATCGCGCTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAg                                                                                                                <  7:89300/149‑1 (MQ=255)
                               aTCACTTCGCTGGCGCTGACATCGCGCTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAg                                                                                                                >  3:173363/1‑149 (MQ=255)
                                tCACTTCGCTGGCGCTGACATCGCGCTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAgg                                                                                                               >  3:280524/1‑149 (MQ=255)
                                       gCTGGCGCTGACATCGCGCTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCctgct                                                                                                        <  1:249207/149‑1 (MQ=255)
                                          ggCGCTGACATCGCGCTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGAc                                                                                                     <  4:173363/149‑1 (MQ=255)
                                            cgcTGACATCGCGCTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGt                                                                                                   <  4:280524/149‑1 (MQ=255)
                                                 aCATCGCGCTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCg                                                                                                <  2:12517/147‑1 (MQ=255)
                                                   aTCGCGCTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCa                                                                                            >  3:353930/1‑149 (MQ=255)
                                                     cgcgCTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCCGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATg                                                                                          >  1:86926/1‑149 (MQ=255)
                                                     cgcgCTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCCGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATg                                                                                          >  1:91920/1‑149 (MQ=255)
                                                      gcgcTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCgg                                                                                                                                        >  6:100211/1‑102 (MQ=255)
                                                      gcgcTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCgg                                                                                                                                        <  5:100211/102‑1 (MQ=255)
                                                       cgcTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGc                                                                                        <  5:193463/149‑1 (MQ=255)
                                                          tCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGc                                                                                     >  2:65367/1‑149 (MQ=255)
                                                           cGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGc                                                                                     >  8:64787/1‑148 (MQ=255)
                                                            gCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCg                                                                                    <  1:65367/148‑1 (MQ=255)
                                                                  gggCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAgg                                                                             >  8:100207/1‑149 (MQ=255)
                                                                        gAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGa                                                                         <  3:156495/147‑1 (MQ=255)
                                                                            ttGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTgcgg                                                                   <  5:163269/149‑1 (MQ=255)
                                                                            ttGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCCGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTgcgg                                                                   <  2:86926/149‑1 (MQ=255)
                                                                            ttGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCCGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTgcgg                                                                   <  2:91920/149‑1 (MQ=255)
                                                                              gATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTgcggcg                                                                 >  1:154055/1‑149 (MQ=255)
                                                                               aTCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGgcgc                                                                >  8:235871/1‑149 (MQ=255)
                                                                               aTCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGgcgc                                                                >  8:230120/1‑149 (MQ=255)
                                                                                        cGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGGCGCTTCGGAAAt                                                       <  7:230120/149‑1 (MQ=255)
                                                                                        cGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGGCGCTTCGGAAAt                                                       <  7:235871/149‑1 (MQ=255)
                                                                                              cTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGAt                                                                                                                            <  3:324990/74‑1 (MQ=255)
                                                                                              cTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGAt                                                                                                                            >  4:324990/1‑74 (MQ=255)
                                                                                               tGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGGCGCTTCGGAAATACCCTg                                                 >  5:203418/1‑148 (MQ=255)
                                                                                               tGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGACTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGGCGCTTCGGAAATACCCTg                                                 >  3:352957/1‑148 (MQ=255)
                                                                                                 ttGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGGCGCTTCGGAAATACCCTGGa                                               <  4:353930/148‑1 (MQ=255)
                                                                                                 ttGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGGCGCTTCGGAAATACCCTGGAt                                              >  3:165322/1‑149 (MQ=255)
                                                                                                   tacattGGCGTAGACGGCGACAACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGGCGCTTCGGAAATACCCTGGATCa                                            >  8:117762/6‑149 (MQ=255)
                                                                                                    acattGGCGTAGACGGCGACAACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGGCGCTTCGGAAATACCCTGGATCAc                                           >  8:340557/5‑149 (MQ=255)
                                                                                                                  gCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGGCGCTTCGGAAATACCCTGGATCACCCCGGTATCCTGCa                             >  3:306336/1‑149 (MQ=255)
                                                                                                                              aaTGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGGCGCTTCGGAAATACCCTGGATCACCCCGGTATCCTGCACCGGGAAGAAGc                 <  4:165322/149‑1 (MQ=255)
                                                                                                                                  tAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGGCGCTTCGGAAATACCCTGGATCACCCCGGTATCCTGCACCGGGAAGAAGCCCtt             >  6:260691/1‑149 (MQ=255)
                                                                                                                                    ggACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGGCGCTTCGGAAATACCCTGGATCACCCCGGTATCCTGCACCGGGAAGAAGCCCTTg            <  2:154055/148‑1 (MQ=255)
                                                                                                                                     gacgacAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGGCGCTTCGGAAATACCCTGGATCACCCCGGTATCCTGCACCGGGAAGAAGCCCTTggg          >  1:41174/1‑149 (MQ=255)
                                                                                                                                     gacgacAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGGCGCTTCGGAAATACCCTGGATCACCCCGGTATCCTGCACCGGGAAGAAGCCCTTgg           <  7:64787/148‑1 (MQ=255)
                                                                                                                                         acAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGGCGCTTCGGAAATACCCTGGATCACCCCGGTATCCTGCACCGGGAAGAAGCCCTTGGGCAc       >  8:234229/1‑148 (MQ=255)
                                                                                                                                             gCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGGCGCTTCGGAAATACCCTGGATCACCCCGGTATCCTGCACCGGGAAGAAGCCCTTGGGCACGACCa  >  5:135693/1‑149 (MQ=255)
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CAGCCGGTCGACCTGCGGCTGCAGGCGGCTGATCACTTCGCTGGCGCTGACATCGCGCTCGCCGTGGGGCTTGAGGTTGATCAGCAGGCGACCACTGTTGAGCGTGGCGTTGTCGCCGTCTACGCCAATGTAGGACGACAGGCTCTGCACGGCCGGGTCTTGCAGGATGACCTTGCTCAGGGCCTGCTGACGTTCGCTCATGGCAGCGAACGAGGTGGACTGCGGCGCTTCGGAAATACCCTGGATCACCCCGGTATCCTGCACCGGGAAGAAGCCCTTGGGCACGACCA  >  NC_002947/4070230‑4070519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑