Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,085,855 T→A 5.3% V185D (GTC→GAC)  amaD → D‑lysine oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,085,8550TA 5.3% 223.7 / 2.7 76V185D (GTC→GAC) amaDD‑lysine oxidase
Reads supporting (aligned to +/- strand):  ref base T (34/38);  new base A (2/2);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

AAAGGCTGGATCGAAGTGTTCCGCGACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGTGCATGGCGATGCGCGCAGCCTGCGCCAGGCCAATGGCCAATGGCGGGTA  >  NC_002947/4085707‑4086003
                                                                                                                                                    |                                                                                                                                                    
aaaGGCTGGATCGAAGTGTTCCGCGACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGt                                                                                                                                                      >  5:377281/1‑149 (MQ=255)
     cTGGATCGAAGTGTTCCGCGACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTg                                                                                                                                                 <  2:124537/149‑1 (MQ=255)
        gATCGAAGTGTTCCGCGACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGc                                                                                                                                               <  8:12434/148‑1 (MQ=255)
        gATCGAAGTGTTCCGCGACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCa                                                                                                                                              <  4:362397/149‑1 (MQ=255)
         aTCGAAGTGTTCCGCGACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCAt                                                                                                                                             <  7:158444/149‑1 (MQ=255)
          tCGAAGTGTTCCGCGACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATc                                                                                                                                            >  3:302409/1‑149 (MQ=255)
          tCGAAGGGTTCCGCGACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATc                                                                                                                                            <  2:292244/149‑1 (MQ=255)
             aaGTGTTCCGCGACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGATGCCACCGACCACGGTGGCGTCCag                                                                                                                                         <  5:219699/149‑2 (MQ=255)
              aGTGTTCCGCGACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGATGCCACCGACCACGGTGGCGTCCag                                                                                                                                         <  6:113703/148‑2 (MQ=255)
               gtgtTCCGCGACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTg                                                                                                                                       <  6:118357/149‑1 (MQ=255)
                    ccGCGACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTc                                                                                                                                   >  4:120556/1‑148 (MQ=255)
                     cgcgACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCg                                                                                                                                  <  4:302409/148‑1 (MQ=255)
                     cgcgACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGa                                                                                                                                 <  1:179002/149‑1 (MQ=255)
                     cgcgACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGa                                                                                                                                 >  7:145672/1‑149 (MQ=255)
                      gcgACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGAc                                                                                                                                >  6:59621/1‑149 (MQ=255)
                      gcgACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGAc                                                                                                                                <  8:145672/149‑1 (MQ=255)
                       cgACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGAcc                                                                                                                               >  2:366498/1‑149 (MQ=255)
                                 cTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGt                                                                                                                     <  7:278971/149‑1 (MQ=255)
                                 cTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGt                                                                                                                     <  8:33550/149‑1 (MQ=255)
                                       gAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGaacaa                                                                                                               >  4:344811/1‑149 (MQ=255)
                                            ggCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTg                                                                                                                                       >  6:268610/1‑120 (MQ=255)
                                            ggCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTg                                                                                                                                       <  5:268610/120‑1 (MQ=255)
                                             gCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCgg                                                                                                         >  7:179409/1‑149 (MQ=255)
                                               aaaaaCCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCATTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCTGTGGCATCCACTGGCTCGACCCGAAAACCCTGAACAACACCGgcg                                                                                                       >  8:163889/1‑149 (MQ=255)
                                                  aaCCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGgcgcg                                                                                                     >  2:206786/1‑148 (MQ=255)
                                                   aCCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCt                                                                                                   <  8:179409/149‑1 (MQ=255)
                                                     cGATTCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGACCATCAGCTGGCCGCCACCGTGGTCGGTGGCATCCACTGGCTCGCCCCGAAAACCGTGAACAACCCAGCCGAGCTCa                                                                                                 >  4:31382/1‑149 (MQ=255)
                                                     cGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCa                                                                                                 >  4:357396/1‑149 (MQ=255)
                                                           cAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAAccc                                                                                                            <  2:268455/132‑1 (MQ=255)
                                                           cAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAAccc                                                                                                            >  1:268455/1‑132 (MQ=255)
                                                           cAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTg                                                                                           <  6:41738/149‑1 (MQ=255)
                                                           cAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTg                                                                                           <  3:120556/149‑1 (MQ=255)
                                                            aaGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTgg                                                                                          <  3:344811/149‑1 (MQ=255)
                                                                     aGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATgcg                                                                                   <  1:206786/147‑1 (MQ=255)
                                                                        cGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCgtt                                                                              >  8:227251/1‑149 (MQ=255)
                                                                          cTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGc                                                                                                                                     >  5:336405/1‑92 (MQ=255)
                                                                          cTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGc                                                                                                                                     <  6:336405/92‑1 (MQ=255)
                                                                             cGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTc                                                                          <  7:163889/148‑1 (MQ=255)
                                                                               gCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTg                                                                       <  4:131281/149‑1 (MQ=255)
                                                                                cTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTg                                                                       >  2:160740/1‑148 (MQ=255)
                                                                                cTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTg                                                                       >  5:176863/1‑148 (MQ=255)
                                                                                 ttGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCgttgtt                                                                           <  1:1098/143‑1 (MQ=255)
                                                                                 ttGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCgttgtt                                                                           >  2:1098/1‑143 (MQ=255)
                                                                                   gcgcTTCGAAATCCTTGAATGCGGTCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACAGTGGTCGGTGGCATCCACTGGCTCGACCCGATAACAGTGAACAACCCCGGCGCGCTCACCCGTGTCTATGCGGCGTTGTTCCTGc                                                                      <  3:31382/146‑1 (MQ=255)
                                                                                   gcgcTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTg                                                                                                                                                 >  6:315226/1‑71 (MQ=255)
                                                                                   gcgcTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTg                                                                                                                                                 <  5:315226/71‑1 (MQ=255)
                                                                                          gAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGcggcgg                                                            >  1:195739/1‑149 (MQ=255)
                                                                                            aaTCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCggg                                                           >  1:182974/1‑148 (MQ=255)
                                                                                            aaTCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCggg                                                           <  2:182974/148‑1 (MQ=255)
                                                                                                 ttGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTc                                                     >  3:280959/1‑149 (MQ=255)
                                                                                                   gAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGt                                                   >  4:361913/1‑149 (MQ=255)
                                                                                                         gggCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGTGCATgg                                             <  6:176863/149‑1 (MQ=255)
                                                                                                             aGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGTGCATGGCGAt                                         <  2:195739/149‑1 (MQ=255)
                                                                                                               ttGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGTGCATGGCGATgc                                       >  6:137206/1‑149 (MQ=255)
                                                                                                               ttGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGTGCATGGCGATgc                                       <  3:357396/149‑1 (MQ=255)
                                                                                                                   aGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTg                                                                                           >  8:251589/1‑93 (MQ=255)
                                                                                                                   aGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTg                                                                                           <  7:251589/93‑1 (MQ=255)
                                                                                                                   aGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGTGCATGGCGATgcgcg                                    >  6:197012/1‑148 (MQ=255)
                                                                                                                    ggCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGTGCATGGCGATGCGCGCa                                  <  5:59621/149‑1 (MQ=255)
                                                                                                                     gCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTgg                                                                                          <  8:355784/92‑1 (MQ=255)
                                                                                                                     gCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTgg                                                                                          >  7:355784/1‑92 (MQ=255)
                                                                                                                     gCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGTGCa                                                <  3:302656/134‑1 (MQ=255)
                                                                                                                     gCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGTGCa                                                >  4:302656/1‑134 (MQ=255)
                                                                                                                     gCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGTGCATGGCGATGCGCGCAg                                 >  2:58601/1‑149 (MQ=255)
                                                                                                                       aCGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGTGCATGGCGATGCGCGCAGcc                               >  6:298540/1‑149 (MQ=255)
                                                                                                                         gTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATgcgg                                                                                  <  3:227765/96‑1 (MQ=255)
                                                                                                                         gTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATgcgg                                                                                  >  4:227765/1‑96 (MQ=255)
                                                                                                                          tGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGTGCATGGCGATGCGCGCAGCCTgc                            <  4:280959/149‑1 (MQ=255)
                                                                                                                                  cagtGGATGCCACCGACCACGGTGGCGTCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGAGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGTGCATGGCGATGCGCGCAGCCTGCGCCAggc                     >  5:198972/4‑148 (MQ=255)
                                                                                                                                   gCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTg                                                                                           <  2:5327/77‑1 (MQ=255)
                                                                                                                                   gCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTg                                                                                           >  1:5327/1‑77 (MQ=255)
                                                                                                                                   agtGGATGCCACCGACCACGGTGGCGTCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGTGCATGGCGATGCGCGCAGCCTGCGCCAggcca                   >  3:270119/3‑149 (MQ=255)
                                                                                                                                       gACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGTAACGCGGCGGGCAGTTCGTGCATGGCGATGCGCGCAGCCTGCGCCAggccaatgg               <  7:227251/149‑1 (MQ=255)
                                                                                                                                       gACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGTGCATGGCGATGCGCGCAGCCTGCGCCAggccaatg                <  5:197012/148‑1 (MQ=255)
                                                                                                                                                 tGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGTGCATGGCGATGCGCGCAGCCTGCGCCAGGCCAATGGCCAATGGCgg     >  1:202606/1‑149 (MQ=255)
                                                                                                                                                  ggTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCgttgt                                                                            <  2:966/77‑1 (MQ=255)
                                                                                                                                                  ggTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCgttgt                                                                            >  1:966/1‑77 (MQ=255)
                                                                                                                                                    tCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGTGCATGGCGATGCGCGCAGCCTGCGCCAGGCCAATGGCCAATGGCGGGTa  <  1:58601/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAAGGCTGGATCGAAGTGTTCCGCGACCCCGCGCTGTTCGAGCAGGCAAAAACCGATGCCAAGGGCCTGAGCCGCTACGGCTTGCGCTTCGAAATCCTTGAATGCGGGCAGTTGCAGGCACGTGAACATCAGCTGGACGCCACCGTGGTCGGTGGCATCCACTGGCTCGACCCGAAAACCGTGAACAACCCCGGCGCGCTCACCCGTGGCTATGCGGCGTTGTTCCTGCAACGCGGCGGGCAGTTCGTGCATGGCGATGCGCGCAGCCTGCGCCAGGCCAATGGCCAATGGCGGGTA  >  NC_002947/4085707‑4086003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑