Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,086,926 G→A 7.4% A120T (GCC→ACC)  PP_3597 → amino acid ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,086,9260GA 7.4% 155.7 / 2.8 54A120T (GCC→ACC) PP_3597amino acid ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (25/25);  new base A (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

GCCCAGCAGGGCAGCATCCAGGTAG‑ACGGCATCGACCTGGCGGCCACCACACGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGCACAAGTACCCCAGCCAGCTGTCCGGTGGCCAGCAGCAGCGCGTGGCAATCGCTCGG  >  NC_002947/4086782‑4087072
                                                                                                                                                 |                                                                                                                                                   
gaccAGCAGGGCAGCATCCAGGTAGAAGGGCATCGACCTGGCGGCCACCACACGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGAACTACAACCTGTACCCGCACATGAGCGTGCTCGACAACTGCCTGCTggtgg                                                                                                                                                  >  1:221477/3‑146 (MQ=255)
 cccAGCAGGGCAGCATCCAGGTAG‑ACGGCATCGACCTGGCGGCCACCACACGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGcccc                                                                                                                                                 >  4:208646/1‑148 (MQ=255)
         ggcgacttCCAGGTAG‑ACGGCATCGACCTGGCGGCCACCACACGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGc                                                                                                                                          <  8:253994/140‑1 (MQ=255)
                  cAGGTAG‑ACGGCATCGACCTGGCGGCCACCACACGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCt                                                                                                                               >  3:354313/1‑149 (MQ=255)
                   aGGTAG‑ACGGCATCGACCTGGCGGCCACCACACGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTg                                                                                                                              <  5:92726/149‑1 (MQ=255)
                   aGGTAG‑ACGGCATCGACCTGGCGGCCACCACACGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTg                                                                                                                              >  4:31343/1‑149 (MQ=255)
                     gTAG‑ACGGCATCGACCTGGCGGCCACCACACGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTc                                                                                                                            <  3:208646/149‑1 (MQ=255)
                        g‑ACGGCATCGACCTGGCGGCCACCACACGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACg                                                                                                                         >  7:219810/1‑149 (MQ=255)
                          aCGGCATCGACCTGGCGGCCACCACACGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACg                                                                                                                         >  6:25870/1‑148 (MQ=255)
                            ggCATCGACCTGGCGGCCACCACACGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCaa                                                                                                                      <  1:159943/149‑1 (MQ=255)
                                    ccTGGCGGCCACCACACGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGcc                                                                                                               >  7:8319/1‑148 (MQ=255)
                                        gcggcCACCACACGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCgag                                                                                                            <  4:354313/147‑1 (MQ=255)
                                         cggcCACCACACGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCgaggag                                                                                                         >  6:380356/1‑149 (MQ=255)
                                          ggcCACCACACGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGc                                                                                                        <  8:8319/149‑1 (MQ=255)
                                            ccaccaCACGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCgg                                                                                                      >  1:260438/1‑149 (MQ=255)
                                              accacACGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGc                                                                                                    <  5:25870/149‑1 (MQ=255)
                                                 acacGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCa                                                                                                                           >  4:9903/1‑123 (MQ=255)
                                                 acacGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCa                                                                                                                           <  3:9903/123‑1 (MQ=255)
                                                      cgAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGt                                                                                            <  3:31343/149‑1 (MQ=255)
                                                            agcaCAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGa                                                                                      <  5:66006/149‑1 (MQ=255)
                                                                   gTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGt                                                                               <  8:219810/149‑1 (MQ=255)
                                                                     aCGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAg                                                                                     >  5:128237/1‑141 (MQ=255)
                                                                     aCGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAg                                                                                     <  6:128237/141‑1 (MQ=255)
                                                                              cATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑Gaaa                                                                   <  1:160488/149‑1 (MQ=255)
                                                                               aTCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAg                                                                  >  8:232330/1‑149 (MQ=255)
                                                                               aTCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAg                                                                  >  8:226421/1‑149 (MQ=255)
                                                                                tCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGc                                                                 <  2:27334/149‑1 (MQ=255)
                                                                                        gtgtTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAgg                                                             >  4:5291/1‑145 (MQ=255)
                                                                                        gtgtTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAgg                                                             <  3:5291/145‑1 (MQ=255)
                                                                                         tgtTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGcac                                                        <  7:252874/149‑1 (MQ=255)
                                                                                         tgtTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGcac                                                        <  2:260438/149‑1 (MQ=255)
                                                                                          gtTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCCCGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGcac                                                        >  1:339345/1‑148 (MQ=255)
                                                                                          gtTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGACGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGcac                                                        >  3:372791/1‑148 (MQ=255)
                                                                                              cAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGc                                                                                                >  8:45154/1‑105 (MQ=255)
                                                                                              cAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGc                                                                                                <  7:45154/105‑1 (MQ=255)
                                                                                               aGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGCACAAGTAc                                                  >  7:130828/1‑149 (MQ=255)
                                                                                                gCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGc                                                                                                                >  1:74880/1‑87 (MQ=255)
                                                                                                gCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGc                                                                                                                <  2:74880/87‑1 (MQ=255)
                                                                                                      ccaCCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGCACAAGTACCccagc                                            <  8:340043/146‑1 (MQ=255)
                                                                                                       aaCCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGACCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑Gaa                                                                    >  2:129521/1‑123 (MQ=255)
                                                                                                       aaCCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGACCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑Gaa                                                                    <  1:129521/123‑1 (MQ=255)
                                                                                                             ttCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGCACAAGTACCCCAGCCAGCTGTc                                    <  8:130828/149‑1 (MQ=255)
                                                                                                                ccGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCgg                                                                                                      <  1:174161/81‑1 (MQ=255)
                                                                                                                ccGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCgg                                                                                                      >  2:174161/1‑81 (MQ=255)
                                                                                                                ccGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGCACAAGTACCCCAGCCAGCTGTCCgg                                 >  2:303121/1‑149 (MQ=255)
                                                                                                                ccGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGCACAAGTACCCCAGCCAGCTGTCCgg                                 >  7:88129/1‑149 (MQ=255)
                                                                                                                             tGCTCGACAACTGCCTGCTGACCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGCACAAGTACCCCAGCCAGCTGTCCGGTGGcc                            <  3:200605/141‑1 (MQ=255)
                                                                                                                             tGCTCGACAACTGCCTGCTGACCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACCCCGAGGAGCGCGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGCACAAGTACCCCACCCAGCTGTCCCGTCGcc                            >  4:200605/1‑141 (MQ=255)
                                                                                                                              gCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGCACAAGTACCCCAGCCAGCTGTCCGGTGGCCagcagcagc                   >  8:261325/1‑149 (MQ=255)
                                                                                                                                  gACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGCACAAGTACCCCAGCCAGCTGTCCGGTGGCCAGCAGCAGCGCGt               >  6:361596/1‑149 (MQ=255)
                                                                                                                                  gACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGCACAAGTACCCCAGCCAGCTGTCCGGTGGCCAGCAGCAGCGCGt               >  2:248865/1‑149 (MQ=255)
                                                                                                                                         gcctgcTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGCACAAGt                                                    <  3:148174/105‑1 (MQ=255)
                                                                                                                                         gcctgcTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGCACAAGt                                                    >  4:148174/1‑105 (MQ=255)
                                                                                                                                          cctgcTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGCACAAGTACCCCAGCCAGCTGTCCGGTGGCCAGCAGCAGCGCGTGGCAAt         <  5:380356/147‑1 (MQ=255)
                                                                                                                                           ctgctgGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATCAGAAAGCCAGGCGCACAAGTACCCCAGCCAGCTGTCCGGTGGCCAGCAGCAGCGCGTGGCAATCg       <  1:303121/149‑1 (MQ=255)
                                                                                                                                                 gCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGCACAAGTACCCCAGCCAGCTGTCCGGTGGCCAGCAGCAGCGCGTGGCAATCGCTCgg  <  1:248865/147‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCCCAGCAGGGCAGCATCCAGGTAG‑ACGGCATCGACCTGGCGGCCACCACACGCGAGGCAGCACAGGTACGCAGCGACATCGGCATGGTGTTCCAGCACTTCAACCTGTTCCCGCACATGAGCGTGCTCGACAACTGCCTGCTGGCCCCCACCAGCGTGCGCGGGCTGTCACGCAAGGACGCCGAGGAGCGGGCACGCATGTACCTGAGCAAAGTCGGCATC‑GAAAGCCAGGCGCACAAGTACCCCAGCCAGCTGTCCGGTGGCCAGCAGCAGCGCGTGGCAATCGCTCGG  >  NC_002947/4086782‑4087072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑