Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,134,699 A→G 6.3% S111P (TCC→CCC)  PP_3638 ← acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,134,6990AG 6.3% 184.8 / 2.4 63S111P (TCC→CCC) PP_3638acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (30/29);  new base G (2/2);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

CGATGGCCGCCGCCACCACGAAGCCGCTTTTACGCAAGTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATGAGCGGCTGG  >  NC_002947/4134558‑4134842
                                                                                                                                             |                                                                                                                                               
cGATGGCCGCCGCCACCACGAAGCCGCTTTTACGCAAGTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCg                                                                                                                                          >  1:347154/1‑149 (MQ=255)
 gATGGCCGCCGCCACCACGAAGCCGCTTTTACGCAAGTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCgg                                                                                                                                         <  2:119700/149‑1 (MQ=255)
      ccgccgccACCACGAAGCCGCTTTTACGCAAGTTGGTCACCCAGTGCAGTAGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCa                                                                                                                                    >  7:7270/1‑149 (MQ=255)
        gccgccACCACGAAGCCGCTTTTACGCAAGTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCAcc                                                                                                                                  <  8:7270/149‑1 (MQ=255)
         ccgccACCACGAAGCCGCTTTTACGCAAGTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCg                                                                                                                                 <  3:84531/149‑1 (MQ=255)
           gccACCACGAAGCCGCTTTTACGCAAGTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCGGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGc                                                                                                                               <  4:124791/149‑1 (MQ=255)
            cctccacGAAGCCGCTTTTACGCAAGTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGGACCTGCAGCGCTTCGATACCGGACAGGAACATCATGGCGTTGGACAGCCCGGTGGCACGGGcc                                                                                                                              <  3:109984/146‑1 (MQ=255)
              accacGAAGCCGCTTTTACGCAAGTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCaa                                                                                                                            <  8:64861/149‑1 (MQ=255)
               ccacGAAGCCGCTTTTACGCAAGTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAAt                                                                                                                           >  5:241888/1‑149 (MQ=255)
                cacGAAGCCGCTTTTACGCAAGTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAAtt                                                                                                                          <  5:376000/149‑1 (MQ=255)
                       ccGCTTTTACGCAAGTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCGCCTGCAGCGCTTCGATACCGGACAGGAACTTCTTGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCgtt                                                                                                                   >  2:114096/1‑147 (MQ=255)
                         gCTTTTACGCAAGTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCg                                                                                                                     <  4:115737/145‑1 (MQ=255)
                         gCTTTTACGCAAGTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCg                                                                                                                     >  3:115737/1‑145 (MQ=255)
                              tACGCAAGTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAg                                                                                                             <  6:241888/148‑1 (MQ=255)
                                 gCAAGTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGACCTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCcgc                                                                                                         >  8:156826/1‑149 (MQ=255)
                                     gTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAAc                                                                                                               <  3:271144/139‑1 (MQ=255)
                                     gTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAAc                                                                                                               <  3:276146/139‑1 (MQ=255)
                                     gTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAAc                                                                                                               >  4:271144/1‑139 (MQ=255)
                                     gTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAAc                                                                                                               >  4:276146/1‑139 (MQ=255)
                                        ggTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGgcagc                                                                                                   <  7:139072/148‑1 (MQ=255)
                                          tCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAgg                                                                                                >  4:320941/1‑149 (MQ=255)
                                                     aGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGGCAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAg                                                                                     >  5:196928/1‑149 (MQ=255)
                                                          cccTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGGCAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCt                                                                                >  8:172866/1‑149 (MQ=255)
                                                           ccTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTg                                                                               <  3:179547/149‑1 (MQ=255)
                                                            cTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTgg                                                                              >  1:25755/1‑149 (MQ=255)
                                                               ccAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTg                                                                           >  4:132009/1‑149 (MQ=255)
                                                                cAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTg                                                                           <  3:320941/148‑1 (MQ=255)
                                                                  ggTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTgggg                                                                        <  2:347154/149‑1 (MQ=255)
                                                                    tGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGGCAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCt                                                                      <  6:196928/149‑1 (MQ=255)
                                                                       cagccaTCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCtgtg                                                                   <  4:171786/149‑1 (MQ=255)
                                                                        agccaTCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCCCCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTgc                                                                  >  5:209848/1‑149 (MQ=255)
                                                                        agccaTCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCtgtg                                                                   >  2:53368/1‑148 (MQ=255)
                                                                        agccaTCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCtgtg                                                                   >  2:376741/1‑148 (MQ=255)
                                                                        agccaTCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTgc                                                                  >  8:287421/1‑149 (MQ=255)
                                                                          ccaTCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTc                                                                                                                  >  6:374072/1‑99 (MQ=255)
                                                                          ccaTCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTc                                                                                                                  <  5:374072/99‑1 (MQ=255)
                                                                           caTCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTgcag                                                                >  7:12540/1‑148 (MQ=255)
                                                                            aTCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTgcag                                                                >  5:269842/1‑147 (MQ=255)
                                                                                  tGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTAtt                                                        <  4:190203/149‑1 (MQ=255)
                                                                                   gCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTc                                                       <  2:90840/149‑1 (MQ=255)
                                                                                    ccGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTc                                                       <  1:53368/148‑1 (MQ=255)
                                                                                    ccGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCg                                                      <  7:92616/149‑1 (MQ=255)
                                                                                     cGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGc                                                                                                                               >  6:368637/1‑75 (MQ=255)
                                                                                     cGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGa                                                     <  5:380570/149‑1 (MQ=255)
                                                                                     cGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGa                                                     <  7:156826/149‑1 (MQ=255)
                                                                                        gcggcCACGCTCCTGCAGCGCTTCGATACCGGACAGGAACTTCCTGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACGTCTCACCAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGa                                                  <  1:114096/149‑1 (MQ=255)
                                                                                         cggcCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATAGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGaa                                                 <  8:228526/149‑1 (MQ=255)
                                                                                             cACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCAGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCa                                             >  8:117913/1‑149 (MQ=255)
                                                                                                      gCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGc                                          <  6:134127/143‑1 (MQ=255)
                                                                                                      gCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGc                                          >  5:134127/1‑143 (MQ=255)
                                                                                                      gCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGccc                                    >  3:126323/1‑149 (MQ=255)
                                                                                                       cAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGccc                                    <  8:12540/148‑1 (MQ=255)
                                                                                                        aGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGccc                                    >  1:343046/1‑147 (MQ=255)
                                                                                                             ttCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGa                             >  6:198236/1‑149 (MQ=255)
                                                                                                             ttCGAAACCGGACAGGAACTTCATGTCGTTGGACAGCCCGGTGGCACAGGCCAATTAACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGa                             >  2:156393/1‑149 (MQ=255)
                                                                                                               cGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGAAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGAc                            >  1:358926/1‑148 (MQ=255)
                                                                                                                          aGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCAGCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGc                <  7:117913/149‑1 (MQ=255)
                                                                                                                            gAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGcc               >  3:219110/1‑148 (MQ=255)
                                                                                                                            gAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGcc               >  3:214151/1‑148 (MQ=255)
                                                                                                                              aCTTCATGGCGTTGGGCAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAAt            <  7:172866/149‑1 (MQ=255)
                                                                                                                                        ggtGGACCGCCCGGGGGCACCGGCCAAGTCACCGGTCAACCGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATGAGCGGCTgg  <  6:269842/147‑1 (MQ=255)
                                                                                                                                        gTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATGAGCGGCTgg  >  1:131833/1‑149 (MQ=255)
                                                                                                                                        gTTCGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCCCGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATGAGCGGCTgg  >  8:253533/1‑149 (MQ=255)
                                                                                                                                             aCAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATg           <  4:306958/135‑1 (MQ=255)
                                                                                                                                             aCAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATg           >  3:306958/1‑135 (MQ=255)
                                                                                                                                             |                                                                                                                                               
CGATGGCCGCCGCCACCACGAAGCCGCTTTTACGCAAGTTGGTCACCCAGTGCAGTCGCCCTTCCAGGTGCCAGCCATCGGCTGCCGGGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATGAGCGGCTGG  >  NC_002947/4134558‑4134842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑