Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,256,904	C→T	9.3%	M1M (ATG→ATA) † *239* (TGA→TAA)	PP_3729 ← aruR ←	amino acid‑binding protein‑like protein transcriptional regulator AruR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,256,904	0	C	T	9.3%	119.1 / 2.7	43	M1M (ATG→ATA) † *239* (TGA→TAA)	PP_3729 aruR	amino acid‑binding protein‑like protein transcriptional regulator AruR
Reads supporting (aligned to +/- strand):  ref base C (19/20);  new base T (2/2);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01

CGGTGGGGGCCAGGCCGCGCACCTGATGGCCGTCGCTCCAGGAAATGGGCGGGTACACCGGGTAGTCGCAGTAGCGCACCGGCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCTTGCGCCGCAGGCGGCCGACCAGCACGTCGATGGAGCGGTCGTTGGGCACCCACTCGCGGT  >  NC_002947/4256756‑4257048                                                                                                                                                     |                                                                                                                                                 cGGTGGGGGCCAGGCCGCGCACCTGATGGCCGTCGCTCCAGGAAATGGGCGGGTACACCGGGTAGTCGCAGTAGCGCACCGGCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATc                                                                                                                                                  <  6:48954/149‑1 (MQ=255)   gTGGGGGCCAGGCCGCGCACCTGATGGCCGTCGCTCCAGGAAATGGGCGGGTACACCGGGTAGTCGCAGTAGCGCACCGGCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCAt                                                                                                                                                <  1:282839/149‑1 (MQ=255)    tGGGGGCCAGGCCGCGCACCTGATGGCCGTCGCTCCAGGAAATGGGCGGGTACACCGGGTAGTCGCAGTAGCGCACCGGCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCAtg                                                                                                                                               <  2:144590/149‑1 (MQ=255)      ggggCCAGGCCGCGCACCTGATGGCCGTCGCTCCAGGAAATGGGCGGGTACACCGGGTAGTCGCAGTAGCGCACCGGCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCAtgct                                                                                                                                             <  3:108622/149‑1 (MQ=255)          ccAGGCCGCGCACATGATGGACGTCGCTCCAGGAAATGGGCGGGTACACCGGGTAGTCGCAGTAGCGCACCGGCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGATACAACTGCAAGGCGAGAGGAATCATGCTGCCa                                                                                                                                         >  2:64314/1‑149 (MQ=255)              gCCGCGCACCTGATGGCCGTCGCTCCAGGAAATGGGCGGGTACACCGGGTAGTCGCAGTAGCGCACCGGCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCt                                                                                                                                     >  3:220369/1‑149 (MQ=255)              gCCGCGCACCTGATGGCCGTCGCTCCAGGAAATGGGCGGGTACACCGGGTAGTCGCAGTAGCGCACCGGCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCt                                                                                                                                     >  8:18468/1‑149 (MQ=255)                     aCCTGATGGCCGTCGCTCCAGGAAATGGGCGGGTACACCGGGTAGTCGCAGTAGCGCACCGGCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTg                                                                                                                              <  4:220369/149‑1 (MQ=255)                          aTGGCCGTCGCTCCAGGAAATGGGCGGGTACACCGGGTAGTCGCAGTAGCGCACCGGCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGTTGCCACGCTGGCGGTGAACAg                                                                                                                         <  5:337050/149‑1 (MQ=255)                                     tCCAGGAAATGGGCGGGTACACCGGGTAGTCGCAGTAGCGCACCGGCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGc                                                                                                              >  1:180542/1‑149 (MQ=255)                                       cAGGAAATGGGCGGGTACACCGGGTAGTCGCAGTAGCGCACCGGCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCg                                                                                                            >  3:242255/1‑149 (MQ=255)                                        aGGAAATGGGCGGGTACACCGGGTAGTCGCAGTAGCGCACCGGCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGt                                                                                                           <  4:18590/149‑1 (MQ=255)                                           ataTGTGCGGGTACACCGGGTAGTCGCTGTAGCGCAGCGGCTCTCTGGCACTGGCGTGCAGGGTGGGCAACGTCATGGCCAGCGCCAACTGCCTGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTTaa                                                                                                        <  3:112654/147‑1 (MQ=255)                                                ggCGGGTACACCGGGTAGTCGCAGTAGCGCACCGGCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGtg                                                                                                   <  2:52434/149‑1 (MQ=255)                                                        cacCGGGTAGTCGCAGAAGCGCACCGGCTCACTGGCACTGGCGTGCACCGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCCAGGAATCATGCAGCCACGCAGGCGGAGAACAGGTAGCCGCTGCCGTGATAGGTGATGAGCaa                                                                                           >  1:187233/1‑149 (MQ=255)                                                                    gCAGTAGCGCACCGGCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGGTCAGGtgcggtgcggg                                                                               >  8:123389/1‑148 (MQ=255)                                                                          gcgcACCGGCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGgtc                                                                         >  2:106675/1‑149 (MQ=255)                                                                            gcACCGGCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGgtcg                                                                        >  8:217281/1‑148 (MQ=255)                                                                                  gCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCa                                                                 <  2:156951/149‑1 (MQ=255)                                                                                      actggcactggcGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCt                                                              <  7:123389/148‑1 (MQ=255)                                                                                      actggcactcgcGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCACGCGCCAGGAATCATGCTGACACCCAGGCGGTGAAAAGCTAGCCGCTGCCGAGAATCCTGATGATCAAATGCGGTTCGGCCGGCTCGTCATCCAGCtt                                                             >  4:113326/1‑149 (MQ=255)                                                                                        tggcactggcGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCTTgc                                                           >  8:148692/1‑149 (MQ=255)                                                                                              tggcGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGt                                                                                                           >  1:313074/1‑95 (MQ=255)                                                                                              tggcGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGt                                                                                                           <  2:313074/95‑1 (MQ=255)                                                                                                gcGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCTTGCGCCGCAg                                                    <  7:18468/148‑1 (MQ=255)                                                                                                gcGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCTTGCGCCGCAg                                                    <  2:180542/148‑1 (MQ=255)                                                                                                 cGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCTTGCGCCGCAggc                                                  <  7:148692/149‑1 (MQ=255)                                                                                                        ggTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCTTGCGCCGCAGGCGGCCGAc                                           <  1:106675/149‑1 (MQ=255)                                                                                                            ggCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATTATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGc                                                                               <  8:304884/109‑1 (MQ=255)                                                                                                            ggCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATTATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGc                                                                               >  7:304884/1‑109 (MQ=255)                                                                                                                 cGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCTTGCGCCGCAGGCGGCCGACCAGCACGTc                                  >  5:286847/1‑149 (MQ=255)                                                                                                                     aaGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCa                                                                 <  2:117028/114‑1 (MQ=255)                                                                                                                     aaGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCa                                                                 >  1:117028/1‑114 (MQ=255)                                                                                                                     aaGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCTTGCGCCGCAGGCGGCCGACCAGCACGTCGATg                              >  3:102015/1‑149 (MQ=255)                                                                                                                        gCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCTTGCGCCGCAGGCGGCCGACCAGCACGTCGATGGAg                           >  3:121024/1‑149 (MQ=255)                                                                                                                         ccAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCTTGCGCCGCAGGCGGCCGACCAGCACGTCGATGGAGc                          >  5:236068/1‑149 (MQ=255)                                                                                                                          cAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCTTGCGCCGCAGGCGGCCGACCAGCACGTCGATGGAGCg                         <  7:217281/149‑1 (MQ=255)                                                                                                                             cgcCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCTTGCGCCGCAGGCGGCCGACCAGCACGTCGATGGAGCGGTc                      >  6:34275/1‑149 (MQ=255)                                                                                                                                cAACTGCCAGGCGCGAGGAATTATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCTTGCGCCGCAGGCGGCCGACCAGCACGTCGATGGAGCGGTCGtt                   >  4:352224/1‑149 (MQ=255)                                                                                                                                 aaCTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCTTGCGCCGCAGGCGGCCGACCAGCACGTCGATGGAGCGGTCGTTg                  >  3:129796/1‑149 (MQ=255)                                                                                                                                  aCTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCTTGCGCCGCAGGCGGCCGACCAGCACGTCGATGGAGCGGTCGTTgg                 <  1:372398/149‑1 (MQ=255)                                                                                                                                      ccAGGCGCGAGGAATTATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCTTGCGCCGCAGGCGGCCGACCAGCACGTCGATGGAGCGGTCGTTGGGCAc             <  3:352224/149‑1 (MQ=255)                                                                                                                                          gcgcGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCTTGCGCCGCAGGCGGCCGACCAGCACGTCGATGGAGCGGTCGTTGGGCACCCAc         <  6:236068/149‑1 (MQ=255)                                                                                                                                                 gAATCATGCTGGCACGCTGGCGGTGATCAGGTAGCCGGTGCGGTGAATGGTGATGATGATCTGCGGTTCGGCGGGGTGGTCATGCTGGTTGCGCCGCTGGCGGCCGAGCAGCTCGTCGATGGAGCGGTCGTTGGGGACCCACTCGGGGt  <  2:187233/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CGGTGGGGGCCAGGCCGCGCACCTGATGGCCGTCGCTCCAGGAAATGGGCGGGTACACCGGGTAGTCGCAGTAGCGCACCGGCTCACTGGCACTGGCGTGCAGGGTGGGCAACGTCAAGGCCAGCGCCAACTGCCAGGCGCGAGGAATCATGCTGCCACGCTGGCGGTGAACAGGTAGCCGGTGCCGTGAATGGTGATGATCAACTGCGGTTCGGCCGGGTCGTCATGCAGCTTGCGCCGCAGGCGGCCGACCAGCACGTCGATGGAGCGGTCGTTGGGCACCCACTCGCGGT  >  NC_002947/4256756‑4257048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑