Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 334,604 A→T 9.2% L28M (TTG→ATG) ‡ PP_0276 ← Cro/CI family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947334,6040AT 9.2% 174.2 / 8.3 66L28M (TTG→ATG) ‡PP_0276Cro/CI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (34/25);  new base T (4/2);  total (39/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.91e-01

ATGTCCATCATGGCGTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTC  >  NC_002947/334459‑334750
                                                                                                                                                 |                                                                                                                                                  
aTGTCCATCATGGCGTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTc                                                                                                                                                 <  1:288397/149‑1 (MQ=255)
  gTCCATCATGGCGTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCa                                                                                                                                               >  1:345851/1‑149 (MQ=255)
    ccATCATGGCGTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCACA‑TTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCAttt                                                                                                                                            >  7:177031/1‑149 (MQ=255)
         atGGCGTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACTTGTCCATTTTCa                                                                                                                                         <  8:261576/148‑1 (MQ=255)
            gCGTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGc                                                                                                                                     >  6:35308/1‑149 (MQ=255)
                 cAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCACA‑TTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCAtttt                                                                                                                               <  8:177031/149‑1 (MQ=255)
                    ggTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGc                                                                                                                             <  5:35308/149‑1 (MQ=255)
                      tGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTg                                                                                                                           >  5:74852/1‑149 (MQ=255)
                       gAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGGTTGGCTGCGGCATCATGCTGTTTGAGGGCCATTTTTTTTATTGAGTGAGTGATGAAAATGGACAAGTGCATTTTGAAGGGCATTTTGCttg                                                                                                                          >  1:294256/1‑147 (MQ=255)
                          tttCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGt                                                                                                                                                  <  6:43449/122‑1 (MQ=255)
                          tttCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGt                                                                                                                                                  >  5:43449/1‑122 (MQ=255)
                          tttCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCgg                                                                                                                       >  2:7005/1‑149 (MQ=255)
                              tcGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATc                                                                                                                   >  6:227913/1‑149 (MQ=255)
                                gATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCAATTTAAAGGCCATTTTGCTGGCGGTATCTg                                                                                                                 >  3:216058/1‑149 (MQ=255)
                                    ttGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGATGCCCATTTTTTTGATGCAGTCAGTGATGAAAATCGACAAGTCCATTTTCAAGGCCATTTTGCTGGGGGTATCTGagtc                                                                                                             >  4:234785/1‑149 (MQ=255)
                                          tttGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCgtgggt                                                                                                       >  7:144783/1‑149 (MQ=255)
                                                aGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGCTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTg                                                                                                 <  5:375330/149‑1 (MQ=255)
                                                     ggATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGaaa                                                                                             <  2:345851/148‑1 (MQ=255)
                                                                    ggTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAAtt                                                                             >  2:101132/1‑149 (MQ=255)
                                                                        aCGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAg                                                                         >  8:219610/1‑149 (MQ=255)
                                                                         cGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAgc                                                                        <  2:294256/149‑1 (MQ=255)
                                                                          gTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGGAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGAGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAgcg                                                                       >  5:127028/1‑149 (MQ=255)
                                                                          gTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCgtgg                                                                                                         >  6:216890/1‑115 (MQ=255)
                                                                          gTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCgtgg                                                                                                         <  5:216890/115‑1 (MQ=255)
                                                                          gTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAgcg                                                                       >  3:213015/1‑149 (MQ=255)
                                                                          gTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAgcg                                                                       >  5:110260/1‑149 (MQ=255)
                                                                          gTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAgcg                                                                       >  4:1964/1‑149 (MQ=255)
                                                                            tCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCaa                                                                                                                                        <  2:325486/82‑1 (MQ=255)
                                                                            tCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCaa                                                                                                                                        >  1:325486/1‑82 (MQ=255)
                                                                               gCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATc                                                                  >  5:96883/1‑149 (MQ=255)
                                                                                           catcatGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCgg                                                      <  1:101132/149‑1 (MQ=255)
                                                                                              catGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCgg                                                      >  2:99699/1‑146 (MQ=255)
                                                                                                tGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGaa                                                 <  3:57659/149‑1 (MQ=255)
                                                                                                tGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGa                                                  >  4:57659/1‑148 (MQ=255)
                                                                                                  cTGTTTGAGGCCCCTTTTTTTGTTGCCGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGGGGTATCTGCGTCGTGGGTGGCGGGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAat                                               <  4:216058/149‑1 (MQ=255)
                                                                                                  cTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAat                                               <  6:110260/149‑1 (MQ=255)
                                                                                                        gAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTa                                          >  3:318552/1‑148 (MQ=255)
                                                                                                            cccATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAAtt                                     >  6:317797/1‑149 (MQ=255)
                                                                                                             ccATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAAttt                                    >  6:86507/1‑149 (MQ=255)
                                                                                                             ccATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAAttt                                    >  3:291357/1‑149 (MQ=255)
                                                                                                             ccATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAAttt                                    >  2:127919/1‑149 (MQ=255)
                                                                                                              cATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTg                                   <  5:86507/149‑1 (MQ=255)
                                                                                                               aTTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGc                                  <  5:227913/149‑1 (MQ=255)
                                                                                                               aTTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGc                                  <  1:99699/149‑1 (MQ=255)
                                                                                                                tttttttGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGc                                  >  6:132813/1‑148 (MQ=255)
                                                                                                                  tttttGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGa                                >  7:107948/1‑148 (MQ=255)
                                                                                                                       gATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATc                          >  2:233842/1‑149 (MQ=255)
                                                                                                                            agtcagtGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAgt                     >  1:139851/1‑149 (MQ=255)
                                                                                                                            agtcagtGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAgt                     >  3:3248/1‑149 (MQ=255)
                                                                                                                              tcagtGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAatat                                             <  2:17826/123‑1 (MQ=255)
                                                                                                                              tcagtGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAgtgt                   <  4:213015/149‑1 (MQ=255)
                                                                                                                              tcagtGATGAAAATGGACAAGTCCATTATCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAatat                                             >  1:17826/1‑123 (MQ=255)
                                                                                                                                agtGATGAAAATGGACAATTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGTAAAGGGCTTTCGTGTAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGtgtg                  <  8:144783/148‑1 (MQ=255)
                                                                                                                                agtGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGtgtg                  <  4:318552/148‑1 (MQ=255)
                                                                                                                                  ttatgaAAATGGACAAGTGCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTTGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGAt               <  3:192287/147‑1 (MQ=255)
                                                                                                                                   gatgaAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGAt               <  2:139851/148‑1 (MQ=255)
                                                                                                                                   cttgaAAATGGACTTGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAAt                                                                              <  8:32648/83‑1 (MQ=255)
                                                                                                                                   cttgaAAATGGACTTGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAAt                                                                              >  7:32648/3‑85 (MQ=255)
                                                                                                                                    ttgaAAATGGACTTGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGAttg             >  3:99050/2‑149 (MQ=255)
                                                                                                                                    ttgaAAATGGACTTGTCAATTTTCAAGGCCATTTTGCTGTCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCTGCTTGCCTAGTCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTTGAtgg             >  4:137672/2‑147 (MQ=255)
                                                                                                                                      gaAAATGGACTTGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGAttggt           >  7:75256/1‑149 (MQ=255)
                                                                                                                                      gaAAATGGAC‑‑GTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGAttggttg         >  8:235233/1‑149 (MQ=255)
                                                                                                                                       aaaaTGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGAttggt           <  7:219610/148‑1 (MQ=255)
                                                                                                                                        aaaTGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGAttggttg         <  1:7005/149‑1 (MQ=255)
                                                                                                                                          aTGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGc       >  3:174531/1‑149 (MQ=255)
                                                                                                                                               cAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTc  <  6:96883/149‑1 (MQ=255)
                                                                                                                                                aaGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGtgtg                  >  7:206272/1‑132 (MQ=255)
                                                                                                                                                aaGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGtgtg                  <  8:206272/132‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ATGTCCATCATGGCGTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTC  >  NC_002947/334459‑334750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑