Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,274,562	C→T	5.9%	G173G (GGC→GGT)	glcD →	glycolate oxidase FAD‑linked subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,274,562	0	C	T	5.9%	203.3 / 2.3	67	G173G (GGC→GGT)	glcD	glycolate oxidase FAD‑linked subunit
Reads supporting (aligned to +/- strand):  ref base C (30/33);  new base T (2/2);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

AACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATCGTCACCGAAG  >  NC_002947/4274417‑4274710                                                                                                                                                  |                                                                                                                                                       aaCCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCt                                                                                                                                                      >  5:162873/1‑148 (MQ=255) aaCCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGCTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCCAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCt                                                                                                                                                      >  3:227989/1‑148 (MQ=255) aaCCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATAGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTg                                                                                                                                                     >  1:275363/1‑149 (MQ=255)  aCCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGa                                                                                                                                                    <  5:207177/149‑1 (MQ=255)     gggCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCg                                                                                                                                                 >  1:155967/1‑149 (MQ=255)         gTACGCAACCTGGCCATCTCCCAGGCCGCCTCACCCCATGGTATGAACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTaca                                                                                                                                             <  8:322953/149‑1 (MQ=255)            cGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGTCAGGCCGTacttga                                                                                                                                          <  8:102311/149‑5 (MQ=255)             gCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAAt                                                                                                                                         <  6:64113/149‑1 (MQ=255)             gCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAAt                                                                                                                                         >  7:145014/1‑149 (MQ=255)                 ccTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGc                                                                                                                                     <  3:153466/149‑1 (MQ=255)                 ccTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGc                                                                                                                                     <  5:53525/149‑1 (MQ=255)                  cTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGAGCt                                                                                                                                    >  8:102951/1‑149 (MQ=255)                       cATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAAGGTCGCCTAAAAGGCCGGAGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAgg                                                                                                                               <  1:88445/149‑1 (MQ=255)                       cATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACAGCCTGACCGTACACAATGTGCTCAAgg                                                                                                                               >  3:127914/1‑149 (MQ=255)                             ccAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTg                                                                                                                             <  2:155967/145‑1 (MQ=255)                             ccAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGa                                                                                                                           <  5:363151/147‑1 (MQ=255)                             ccAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACa                                                                                                                         >  7:114333/1‑149 (MQ=255)                             ccAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCAGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACa                                                                                                                         >  5:384260/1‑149 (MQ=255)                             ccAGGCCGCCGCACCCCATGGTATGTACTACGCACCCCACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTg                                                                                                                             <  5:327124/145‑1 (MQ=255)                              cAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACAt                                                                                                                        <  7:192866/149‑1 (MQ=255)                                ggCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATcc                                                                                                                      <  2:15817/149‑1 (MQ=255)                                   cgccgcACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTa                                                                                                                   <  8:145014/149‑1 (MQ=255)                                   cgccgcACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTa                                                                                                                   >  1:263504/1‑149 (MQ=255)                                     ccgcACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACg                                                                                                                 >  5:359295/1‑149 (MQ=255)                                          ccccATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGa                                                                                                          <  7:102951/149‑1 (MQ=255)                                          ccccATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGa                                                                                                          <  6:162873/149‑1 (MQ=255)                                           cccATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAg                                                                                                         <  3:125929/149‑1 (MQ=255)                                            ccttGGTATGTACAAGGCACGCGACCCTTCCTCGCAAATTGCCTTCTCGCTCGGTGGCAAGGTGGCAGAAAAGGCCGGTTGCGTGCAGTGCCTAAAGTACGGCCTGACGGTACAAAATGTGCTCAAGGTGGACTTCGTTAATGT‑‑CGAgg                                                                                                        <  6:384260/146‑1 (MQ=255)                                                        ctacGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGa                                                                                            <  2:263504/149‑1 (MQ=255)                                                         tacGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGAc                                                                                                                          >  2:295094/1‑120 (MQ=255)                                                         tacGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGAc                                                                                                                          <  1:295094/120‑1 (MQ=255)                                                         tacGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAg                                                                                           >  4:296674/1‑149 (MQ=255)                                                          acGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGc                                                                                          >  8:5152/1‑149 (MQ=255)                                                          acGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGc                                                                                          >  2:162218/1‑149 (MQ=255)                                                            gCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCt                                                                                        >  3:40762/1‑149 (MQ=255)                                                               cccGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTggg                                                                                     <  2:275363/149‑1 (MQ=255)                                                                       ttCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAg                                                                                                         <  3:269799/121‑1 (MQ=255)                                                                       ttCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAg                                                                                                         >  4:269799/1‑121 (MQ=255)                                                                           tCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTg                                                                                       >  8:45805/1‑135 (MQ=255)                                                                           tCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTg                                                                                       <  7:45805/135‑1 (MQ=255)                                                                             gCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCt                                                                         <  4:227989/147‑1 (MQ=255)                                                                             gCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTgg                                                                       <  6:359295/149‑1 (MQ=255)                                                                                     acTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTACTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGcccc                                                               >  5:353054/2‑149 (MQ=255)                                                                                      cTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGAc                                                                     <  5:84277/142‑1 (MQ=255)                                                                                      cTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGAc                                                                     >  6:84277/1‑142 (MQ=255)                                                                                      cTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCg                                                              >  1:116375/1‑149 (MQ=255)                                                                                           cGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGAACTGCCTCAAGTACGGCCTGACCGTACAAAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTc                                                         >  4:335333/1‑149 (MQ=255)                                                                                                     cAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTgg                                               <  8:114333/149‑1 (MQ=255)                                                                                                      aaCGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTgg                                               >  6:68084/1‑148 (MQ=255)                                                                                                       aCGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCa                                             >  3:328836/1‑149 (MQ=255)                                                                                                         gTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCAtt                                           <  4:40762/149‑1 (MQ=255)                                                                                                            gCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGtt                                        >  2:208892/1‑149 (MQ=255)                                                                                                             ccGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTc                                       <  1:162218/149‑1 (MQ=255)                                                                                                                       cGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGTTAGCGAGGGCGAACGCGTGAGCATGGGCAGCGAGGCCGGGGCCCGCCCCGGGTTCGACGGGGGGGCATTTTACAGCGGCTc                               >  1:307444/1‑149 (MQ=255)                                                                                                                            gCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGc                        <  4:328836/149‑1 (MQ=255)                                                                                                                               tGCACTGCCTCAAGTACGGCCTGACCGTACACAATGAGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCACCGATGCCCTGGACAGCCACGGCTTCGACCTCCTGGCATTGTTCATCGGCTCCGAAGGCATg                     >  3:232584/1‑149 (MQ=255)                                                                                                                                   cTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCg                 <  3:335333/149‑1 (MQ=255)                                                                                                                                      ccTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGt               <  5:68084/148‑1 (MQ=255)                                                                                                                                        ttgtgTACGGTCAGGCCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATc            >  5:166082/5‑149 (MQ=255)                                                                                                                                        ttgtgTACGGTCAGGCCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATc            <  6:166082/145‑1 (MQ=255)                                                                                                                                         cAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATCg           >  3:133073/1‑149 (MQ=255)                                                                                                                                         cAAGTACGGCCTGACCGTACACAATGCGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCg                 <  6:323652/143‑1 (MQ=255)                                                                                                                                         cAAGTACGGCCTGACCGTACACAATGCGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCg                 >  5:323652/1‑143 (MQ=255)                                                                                                                                          aaGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATc            <  3:296674/147‑1 (MQ=255)                                                                                                                                           tgTACGGTCAGGCCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATCGTc         >  7:266098/2‑149 (MQ=255)                                                                                                                                            gTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATCGTCa        >  2:186716/1‑149 (MQ=255)                                                                                                                                                 gCCTGACCGTACACAATGTACTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCAACGGCTCCGAAGGCATGCTCGGTATCGTCACCGaa   <  6:353054/149‑1 (MQ=255)                                                                                                                                                  ccTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATCGGCACCGAAg  >  8:344021/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                       AACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATCGTCACCGAAG  >  NC_002947/4274417‑4274710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑