Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,274,564	T→A	6.0%	L174Q (CTG→CAG)	glcD →	glycolate oxidase FAD‑linked subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,274,564	0	T	A	6.0%	203.5 / 2.5	66	L174Q (CTG→CAG)	glcD	glycolate oxidase FAD‑linked subunit
Reads supporting (aligned to +/- strand):  ref base T (29/33);  new base A (2/2);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

AACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATCGTCACCGAAGT  >  NC_002947/4274417‑4274711                                                                                                                                                    |                                                                                                                                                      aaCCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCt                                                                                                                                                       >  5:162873/1‑148 (MQ=255) aaCCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGCTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCCAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCt                                                                                                                                                       >  3:227989/1‑148 (MQ=255) aaCCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATAGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTg                                                                                                                                                      >  1:275363/1‑149 (MQ=255)  aCCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGa                                                                                                                                                     <  5:207177/149‑1 (MQ=255)     gggCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCg                                                                                                                                                  >  1:155967/1‑149 (MQ=255)         gTACGCAACCTGGCCATCTCCCAGGCCGCCTCACCCCATGGTATGAACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTaca                                                                                                                                              <  8:322953/149‑1 (MQ=255)            cGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGTCAGGCCGTacttga                                                                                                                                           <  8:102311/149‑5 (MQ=255)             gCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAAt                                                                                                                                          <  6:64113/149‑1 (MQ=255)             gCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAAt                                                                                                                                          >  7:145014/1‑149 (MQ=255)                 ccTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGc                                                                                                                                      <  3:153466/149‑1 (MQ=255)                 ccTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGc                                                                                                                                      <  5:53525/149‑1 (MQ=255)                  cTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGAGCt                                                                                                                                     >  8:102951/1‑149 (MQ=255)                       cATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAAGGTCGCCTAAAAGGCCGGAGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAgg                                                                                                                                <  1:88445/149‑1 (MQ=255)                       cATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACAGCCTGACCGTACACAATGTGCTCAAgg                                                                                                                                >  3:127914/1‑149 (MQ=255)                             ccAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTg                                                                                                                              <  2:155967/145‑1 (MQ=255)                             ccAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGa                                                                                                                            <  5:363151/147‑1 (MQ=255)                             ccAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACa                                                                                                                          >  7:114333/1‑149 (MQ=255)                             ccAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCAGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACa                                                                                                                          >  5:384260/1‑149 (MQ=255)                             ccAGGCCGCCGCACCCCATGGTATGTACTACGCACCCCACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTg                                                                                                                              <  5:327124/145‑1 (MQ=255)                              cAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACAt                                                                                                                         <  7:192866/149‑1 (MQ=255)                                ggCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATcc                                                                                                                       <  2:15817/149‑1 (MQ=255)                                   cgccgcACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTa                                                                                                                    <  8:145014/149‑1 (MQ=255)                                   cgccgcACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTa                                                                                                                    >  1:263504/1‑149 (MQ=255)                                     ccgcACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACg                                                                                                                  >  5:359295/1‑149 (MQ=255)                                          ccccATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGa                                                                                                           <  7:102951/149‑1 (MQ=255)                                          ccccATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGa                                                                                                           <  6:162873/149‑1 (MQ=255)                                           cccATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAg                                                                                                          <  3:125929/149‑1 (MQ=255)                                            ccttGGTATGTACAAGGCACGCGACCCTTCCTCGCAAATTGCCTTCTCGCTCGGTGGCAAGGTGGCAGAAAAGGCCGGTTGCGTGCAGTGCCTAAAGTACGGCCTGACGGTACAAAATGTGCTCAAGGTGGACTTCGTTAATGT‑‑CGAgg                                                                                                         <  6:384260/146‑1 (MQ=255)                                                        ctacGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGa                                                                                             <  2:263504/149‑1 (MQ=255)                                                         tacGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGAc                                                                                                                           >  2:295094/1‑120 (MQ=255)                                                         tacGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGAc                                                                                                                           <  1:295094/120‑1 (MQ=255)                                                         tacGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAg                                                                                            >  4:296674/1‑149 (MQ=255)                                                          acGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGc                                                                                           >  8:5152/1‑149 (MQ=255)                                                          acGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGc                                                                                           >  2:162218/1‑149 (MQ=255)                                                            gCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCt                                                                                         >  3:40762/1‑149 (MQ=255)                                                               cccGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTggg                                                                                      <  2:275363/149‑1 (MQ=255)                                                                       ttCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAg                                                                                                          >  4:269799/1‑121 (MQ=255)                                                                       ttCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAg                                                                                                          <  3:269799/121‑1 (MQ=255)                                                                           tCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTg                                                                                        <  7:45805/135‑1 (MQ=255)                                                                           tCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTg                                                                                        >  8:45805/1‑135 (MQ=255)                                                                             gCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCt                                                                          <  4:227989/147‑1 (MQ=255)                                                                             gCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTgg                                                                        <  6:359295/149‑1 (MQ=255)                                                                                     acTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTACTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGcccc                                                                >  5:353054/2‑149 (MQ=255)                                                                                      cTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGAc                                                                      >  6:84277/1‑142 (MQ=255)                                                                                      cTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGAc                                                                      <  5:84277/142‑1 (MQ=255)                                                                                      cTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCg                                                               >  1:116375/1‑149 (MQ=255)                                                                                           cGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGAACTGCCTCAAGTACGGCCTGACCGTACAAAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTc                                                          >  4:335333/1‑149 (MQ=255)                                                                                                     cAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTgg                                                <  8:114333/149‑1 (MQ=255)                                                                                                      aaCGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTgg                                                >  6:68084/1‑148 (MQ=255)                                                                                                       aCGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCa                                              >  3:328836/1‑149 (MQ=255)                                                                                                         gTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCAtt                                            <  4:40762/149‑1 (MQ=255)                                                                                                            gCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGtt                                         >  2:208892/1‑149 (MQ=255)                                                                                                             ccGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTc                                        <  1:162218/149‑1 (MQ=255)                                                                                                                       cGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGTTAGCGAGGGCGAACGCGTGAGCATGGGCAGCGAGGCCGGGGCCCGCCCCGGGTTCGACGGGGGGGCATTTTACAGCGGCTc                                >  1:307444/1‑149 (MQ=255)                                                                                                                            gCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGc                         <  4:328836/149‑1 (MQ=255)                                                                                                                               tGCACTGCCTCAAGTACGGCCTGACCGTACACAATGAGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCACCGATGCCCTGGACAGCCACGGCTTCGACCTCCTGGCATTGTTCATCGGCTCCGAAGGCATg                      >  3:232584/1‑149 (MQ=255)                                                                                                                                   cTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCg                  <  3:335333/149‑1 (MQ=255)                                                                                                                                      ccTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGt                <  5:68084/148‑1 (MQ=255)                                                                                                                                        ttgtgTACGGTCAGGCCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATc             >  5:166082/5‑149 (MQ=255)                                                                                                                                        ttgtgTACGGTCAGGCCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATc             <  6:166082/145‑1 (MQ=255)                                                                                                                                         cAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATCg            >  3:133073/1‑149 (MQ=255)                                                                                                                                         cAAGTACGGCCTGACCGTACACAATGCGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCg                  <  6:323652/143‑1 (MQ=255)                                                                                                                                         cAAGTACGGCCTGACCGTACACAATGCGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCg                  >  5:323652/1‑143 (MQ=255)                                                                                                                                          aaGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATc             <  3:296674/147‑1 (MQ=255)                                                                                                                                           tgTACGGTCAGGCCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATCGTc          >  7:266098/2‑149 (MQ=255)                                                                                                                                            gTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATCGTCa         >  2:186716/1‑149 (MQ=255)                                                                                                                                                 gCCTGACCGTACACAATGTACTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCAACGGCTCCGAAGGCATGCTCGGTATCGTCACCGaa    <  6:353054/149‑1 (MQ=255)                                                                                                                                                  ccTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATCGGCACCGAAg   >  8:344021/1‑149 (MQ=255)                                                                                                                                                    tGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATCGTCACCGAAGt  >  5:374613/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                      AACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGCCTGACCGTACACAATGTGCTCAAGGTGGACATCCTTACGGT‑‑CGAGGGCGAACGCCTGAGCCTGGGCAGCGATGCCCTGGACAGCCCCGGCTTCGACCTGCTGGCATTGTTCATCGGCTCCGAAGGCATGCTCGGTATCGTCACCGAAGT  >  NC_002947/4274417‑4274711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑