Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,363,971 A→G 8.9% intergenic (+46/‑28) adhP → / → PP_3840 alcohol dehydrogenase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,363,9710AG 8.9% 157.0 / 5.1 56intergenic (+46/‑28)adhP/PP_3840alcohol dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (24/27);  new base G (2/3);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

CCAAGGTAACGCTGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTCTGCTCAGC  >  NC_002947/4363825‑4364112
                                                                                                                                                  |                                                                                                                                             
ccAAGGTAACGCTGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGAcc                                                                                                                                             >  6:76868/1‑149 (MQ=255)
  aaGGTAACGCTGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCct                                                                                                                                           >  7:67893/1‑149 (MQ=255)
    ggTAACGCTGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATAAAGTTCGAAGGCTGACATGCCGAGCCAGGTCATGTGGAACCACAGCAAATCACGTAGATGAccatat                                                                                                                                         >  7:354393/1‑145 (MQ=255)
        aCGCTGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCg                                                                                                                                      <  3:326434/148‑1 (MQ=255)
            tGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCtt                                                                                                                                 >  1:151826/1‑149 (MQ=255)
               gACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTa                                                                                                                              <  7:223001/149‑1 (MQ=255)
               gACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGCAGAGGGTCATCTACGGGCtttgc                                                                                                                              <  4:239002/149‑3 (MQ=255)
                     tCGAGGATATGAAACATATCTTTGACGAGATGATGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCctt                                                                                                                         <  8:354393/148‑1 (MQ=255)
                     tCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCcttc                                                                                                                        <  8:222933/149‑1 (MQ=255)
                       gAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGCAGAGGGTCATCTACGGGCtttgctg                                                                                                                            >  3:77037/1‑139 (MQ=38)
                       gAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGCAGAGGGTCATCTACGGGCtttgctg                                                                                                                            <  4:77037/143‑5 (MQ=38)
                       gAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGAAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCcttct                                                                                                                       >  5:225385/1‑148 (MQ=255)
                        aGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTa                                                                                                                     <  5:311908/149‑1 (MQ=255)
                        aGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTATGCGGGCTTTTAGCcttctt                                                                                                                      >  5:105023/1‑148 (MQ=255)
                         ggATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTaa                                                                                                                    >  7:201871/1‑149 (MQ=255)
                           atatCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAAtt                                                                                                                  <  1:33752/149‑1 (MQ=255)
                                         tttCACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGAAGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGa                                                                                                    >  5:264003/1‑149 (MQ=255)
                                           tGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACa                                                                                                      <  5:117111/145‑1 (MQ=255)
                                              cGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCAt                                                                                                >  6:13355/1‑148 (MQ=255)
                                               gagaTGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGa                                                                                              <  5:76868/149‑1 (MQ=255)
                                                    gCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTc                                                                                         >  5:257938/1‑149 (MQ=255)
                                                      tGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGa                                                                                       >  8:241742/1‑149 (MQ=255)
                                                         aGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTg                                                                                    <  7:241742/149‑1 (MQ=255)
                                                          gggCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGTAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTgg                                                                                   <  1:290765/149‑1 (MQ=255)
                                                                gATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGcc                                                                             >  5:79051/1‑149 (MQ=255)
                                                                  tCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCgg                                                                           >  8:30776/1‑149 (MQ=255)
                                                                    aaaGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCggg                                                                          <  6:257938/148‑1 (MQ=255)
                                                                                gTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCa                                                             <  6:283279/149‑1 (MQ=255)
                                                                                        gTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACACTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTcc                                                      >  6:195757/1‑148 (MQ=255)
                                                                                            gtctgCTGACAGGCTGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGCAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTgg                                                 <  6:264003/145‑1 (MQ=255)
                                                                                            gAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGcc                                                                                                                           <  7:285392/75‑1 (MQ=255)
                                                                                            gAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGcc                                                                                                                           >  8:285392/1‑75 (MQ=255)
                                                                                            gAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAAtt                                                                                                                  >  4:185781/1‑84 (MQ=255)
                                                                                            gAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAAtt                                                                                                                  <  3:185781/84‑1 (MQ=255)
                                                                                             aaGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGAt                                                                     >  3:267109/1‑128 (MQ=255)
                                                                                             aaGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGAt                                                                     <  4:267109/128‑1 (MQ=255)
                                                                                                gCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGTAAAAGACCATGACCGTCGACTGTCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACaa                                             <  6:105023/149‑1 (MQ=255)
                                                                                                        gCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTAcc                                     >  8:352359/1‑149 (MQ=255)
                                                                                                              ccAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGa                                                          <  6:186715/122‑1 (MQ=255)
                                                                                                              ccAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGa                                                          >  5:186715/1‑122 (MQ=255)
                                                                                                               cAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGATCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAacac                              <  7:246081/149‑1 (MQ=255)
                                                                                                                    cATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCAc                                                                                <  4:270833/94‑1 (MQ=255)
                                                                                                                    cATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCAc                                                                                >  3:270833/1‑94 (MQ=255)
                                                                                                                     aTGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGaa                        <  6:79051/149‑1 (MQ=255)
                                                                                                                          taaCCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTc                   <  4:203799/148‑1 (MQ=255)
                                                                                                                             ccACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGc                                           <  1:181383/122‑1 (MQ=255)
                                                                                                                             ccACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGc                                           >  2:181383/1‑122 (MQ=255)
                                                                                                                              cacaGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGt                 >  4:189425/1‑147 (MQ=255)
                                                                                                                               ggctaaaaGCCCGCAGAGGGTCATCTACGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACaa                                             >  6:209575/6‑118 (MQ=37)
                                                                                                                               ggctaaaaGCCCGCAGAGGGTCATCTACGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACaa                                             <  5:209575/113‑1 (MQ=37)
                                                                                                                                 aGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGtt            >  7:43735/1‑149 (MQ=255)
                                                                                                                                  gCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCTATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGttt           <  5:195757/149‑1 (MQ=255)
                                                                                                                                  gCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGttt           <  8:43735/149‑1 (MQ=255)
                                                                                                                                      aGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGAt                                                                     <  4:376298/87‑1 (MQ=255)
                                                                                                                                      aGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGAt                                                                     >  3:376298/1‑87 (MQ=255)
                                                                                                                                           gTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTCTGCTCAGc  <  8:201871/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
CCAAGGTAACGCTGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTCTGCTCAGC  >  NC_002947/4363825‑4364112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑