Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,363,978 G→A 9.2% intergenic (+53/‑21) adhP → / → PP_3840 alcohol dehydrogenase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,363,9780GA 9.2% 147.5 / 5.4 54intergenic (+53/‑21)adhP/PP_3840alcohol dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (22/27);  new base A (2/3);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

ACGCTGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTCTGCTCAGC  >  NC_002947/4363833‑4364112
                                                                                                                                                 |                                                                                                                                      
aCGCTGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCg                                                                                                                                      <  3:326434/148‑1 (MQ=255)
    tGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCtt                                                                                                                                 >  1:151826/1‑149 (MQ=255)
       gACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTa                                                                                                                              <  7:223001/149‑1 (MQ=255)
       gACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGCAGAGGGTCATCTACGGGCtttgc                                                                                                                              <  4:239002/149‑3 (MQ=255)
             tCGAGGATATGAAACATATCTTTGACGAGATGATGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCctt                                                                                                                         <  8:354393/148‑1 (MQ=255)
             tCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCcttc                                                                                                                        <  8:222933/149‑1 (MQ=255)
               gAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGCAGAGGGTCATCTACGGGCtttgctg                                                                                                                            >  3:77037/1‑139 (MQ=38)
               gAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGCAGAGGGTCATCTACGGGCtttgctg                                                                                                                            <  4:77037/143‑5 (MQ=38)
               gAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGAAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCcttct                                                                                                                       >  5:225385/1‑148 (MQ=255)
                aGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTa                                                                                                                     <  5:311908/149‑1 (MQ=255)
                aGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTATGCGGGCTTTTAGCcttctt                                                                                                                      >  5:105023/1‑148 (MQ=255)
                 ggATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTaa                                                                                                                    >  7:201871/1‑149 (MQ=255)
                   atatCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAAtt                                                                                                                  <  1:33752/149‑1 (MQ=255)
                                 tttCACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGAAGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGa                                                                                                    >  5:264003/1‑149 (MQ=255)
                                   tGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACa                                                                                                      <  5:117111/145‑1 (MQ=255)
                                      cGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCAt                                                                                                >  6:13355/1‑148 (MQ=255)
                                       gagaTGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGa                                                                                              <  5:76868/149‑1 (MQ=255)
                                            gCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTc                                                                                         >  5:257938/1‑149 (MQ=255)
                                              tGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGa                                                                                       >  8:241742/1‑149 (MQ=255)
                                                 aGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTg                                                                                    <  7:241742/149‑1 (MQ=255)
                                                  gggCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGTAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTgg                                                                                   <  1:290765/149‑1 (MQ=255)
                                                        gATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGcc                                                                             >  5:79051/1‑149 (MQ=255)
                                                          tCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCgg                                                                           >  8:30776/1‑149 (MQ=255)
                                                            aaaGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCggg                                                                          <  6:257938/148‑1 (MQ=255)
                                                                        gTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCa                                                             <  6:283279/149‑1 (MQ=255)
                                                                                gTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACACTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTcc                                                      >  6:195757/1‑148 (MQ=255)
                                                                                    gtctgCTGACAGGCTGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGCAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTgg                                                 <  6:264003/145‑1 (MQ=255)
                                                                                    gAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGcc                                                                                                                           <  7:285392/75‑1 (MQ=255)
                                                                                    gAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGcc                                                                                                                           >  8:285392/1‑75 (MQ=255)
                                                                                    gAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAAtt                                                                                                                  >  4:185781/1‑84 (MQ=255)
                                                                                    gAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAAtt                                                                                                                  <  3:185781/84‑1 (MQ=255)
                                                                                     aaGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGAt                                                                     <  4:267109/128‑1 (MQ=255)
                                                                                     aaGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGAt                                                                     >  3:267109/1‑128 (MQ=255)
                                                                                        gCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGTAAAAGACCATGACCGTCGACTGTCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACaa                                             <  6:105023/149‑1 (MQ=255)
                                                                                                gCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTAcc                                     >  8:352359/1‑149 (MQ=255)
                                                                                                      ccAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGa                                                          <  6:186715/122‑1 (MQ=255)
                                                                                                      ccAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGa                                                          >  5:186715/1‑122 (MQ=255)
                                                                                                       cAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGATCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAacac                              <  7:246081/149‑1 (MQ=255)
                                                                                                            cATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCAc                                                                                <  4:270833/94‑1 (MQ=255)
                                                                                                            cATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCAc                                                                                >  3:270833/1‑94 (MQ=255)
                                                                                                             aTGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGaa                        <  6:79051/149‑1 (MQ=255)
                                                                                                                  taaCCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTc                   <  4:203799/148‑1 (MQ=255)
                                                                                                                     ccACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGc                                           <  1:181383/122‑1 (MQ=255)
                                                                                                                     ccACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGc                                           >  2:181383/1‑122 (MQ=255)
                                                                                                                      cacaGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGt                 >  4:189425/1‑147 (MQ=255)
                                                                                                                       ggctaaaaGCCCGCAGAGGGTCATCTACGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACaa                                             >  6:209575/6‑118 (MQ=37)
                                                                                                                       ggctaaaaGCCCGCAGAGGGTCATCTACGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACaa                                             <  5:209575/113‑1 (MQ=37)
                                                                                                                         aGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGtt            >  7:43735/1‑149 (MQ=255)
                                                                                                                          gCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCTATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGttt           <  5:195757/149‑1 (MQ=255)
                                                                                                                          gCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGttt           <  8:43735/149‑1 (MQ=255)
                                                                                                                              aGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGAt                                                                     <  4:376298/87‑1 (MQ=255)
                                                                                                                              aGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGAt                                                                     >  3:376298/1‑87 (MQ=255)
                                                                                                                                   gTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTCTGCTCAGc  <  8:201871/149‑1 (MQ=255)
                                                                                                                                                tGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACAATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTCTGCTCAGc  >  3:199516/1‑136 (MQ=255)
                                                                                                                                                 |                                                                                                                                      
ACGCTGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTCTGCTCAGC  >  NC_002947/4363833‑4364112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑